Anti-HIV effect of beta subunit of human chorionic gonadotropin (βhCG) in vitro

Bourinbaiar, Aldar S.; Lee‐Huang, Sylvia

doi:10.1016/0165-2478(94)00191-s

Cited by 35 publications

(17 citation statements)

References 41 publications

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“…Recently, it was shown that hCG can suppress HIV-1 reverse transcriptase activity in chronically infected cells (i.e., lymphocytes and monocytes) and can block viral transmission resulting from cell-to-cell contact between virus-carrying lymphocytes and placental trophoblasts (31,32). It also has been reported that the ␤ subunit of hCG can inhibit p24 gag protein synthesis in virus-producing ACH-2 lymphocytes and U1 monocytes (33). In addition, Lunardi-Iskander et al, demonstrated that the ␤ subunit of hCG can destroy Kaposi's sarcoma-derived cells for AIDS patients and can inhibit tumor growth by these cell lines in nude mice (34).…”

Section: Discussionmentioning

confidence: 99%

Human chorionic gonadotropin hormone prevents wasting syndrome and death in HIV-1 transgenic mice.

Wohlenberg²,

Marinos³

et al. 1997

J. Clin. Invest.

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At birth, transgenic mice, homozygous for the HIV-1 provirus pNL4-3, deleted in gag/pol, are normal in appearance and weight. Within several days after birth, the pups develop a syndrome characterized by dry, scaly, hyperkeratotic skin, growth failure, and death. The possibility that the homozygous embryos are being protected during gestation by a maternal factor led us to treat the newborn animals with various pregnancy-related hormones including human chorionic gonadotropin (hCG), estrogen, progesterone, and dexamethasone. Treatment with hCG prevented death, led to normal growth, and markedly reduced skin lesions. In contrast to the skin of the untreated homozygous pups, which expressed high levels of HIV mRNA and proteins (i.e., gp120 and Nef), the skin of the hCG-treated pups showed a marked reduction in both HIV mRNA and proteins. Discontinuation of hCG resulted in the reappearance of HIV transcripts and proteins, skin lesions, and growth failure resulting in death. In addition, HIV transcripts and proteins were reduced significantly in heterozygous mothers during pregnancy, but reappeared after parturition. Similarly, hCG treatment resulted in a decrease of HIV proteins in the skin of nonpregnant heterozygous transgenic mice. These findings suggest that the inhibiting effect of hCG on HIV expression may be clinically useful in the treatment of HIV infections, and may be responsible, during pregnancy, for the low transmission of HIV from infected mothers to their offspring. (

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Section: Discussionmentioning

confidence: 99%

Human chorionic gonadotropin hormone prevents wasting syndrome and death in HIV-1 transgenic mice.

Wohlenberg²,

Marinos³

et al. 1997

J. Clin. Invest.

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“…42 Chemokines and cytokines with an effective anti-HIV-1 activity have been detected in human placentas. 42,43 Placental cytokines and chemokines may influence HIV replication in trophoblasts. Alternatively, cytokines, such as inflammatory cytokines, facilitate HIV infection and replication during pregnancy.…”

Section: Chemokinesmentioning

confidence: 99%

“…41 Human chorionic gonadotropin (hCG) has been shown in vitro to inhibit reverse transcriptase and to block viral transmission between virus-carrying lymphocytes and placental trophoblasts. 42 However, role of hCG in protecting the fetus from vertical transmission HIV-1 needs to be studied. In summary, the restricted heterogeneity of HIV-1 in the infected mothers is more likely associated with lack of vertical transmission.…”

mentioning

confidence: 99%

Role of placenta in the vertical transmission of human immunodeficiency virus

2008

J Perinatol

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“…More recently it has been reported to inhibit human immunodeficiency virus (HIV) replication in maternal blood cells during pregnancy and transmission from lymphocytes to trophoblasts (1). It was later proposed that the antiviral activity of hCG in blood cells was determined by its free ␤-subunit (hCG␤) (2). The anti-HIV effects of hCG and hCG␤ have also been noted in HIV-associated Kaposi's sarcoma (KS) (3)(4)(5)(6)(7).…”

Section: Human Chorionic Gonadotrophin (Hcg)mentioning

confidence: 92%

Identification of Placental Transforming Growth Factor-β and Bikunin Metabolites as Contaminants of Pharmaceutical Human Chorionic Gonadotrophin Preparations by Proteomic Techniques

Malatos

Neubert

Kicman

et al. 2005

Molecular & Cellular Proteomics

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A contaminant protein complex found in pharmaceutical urinary human chorionic gonadotrophin preparations is reported to have anti-human immunodeficiency virus-associated Kaposi's sarcoma activity. The aim of this study was to isolate and characterize this protein complex by proteomic approaches. Size exclusion chromatography was used in the isolation of these human chorionic gonadotrophin-associated fragments. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed the presence of a protein complex that dissociated into two protein bands under reducing conditions. Matrix-assisted laser desorption/ionization timeof-flight mass spectrometry of this complex showed three polypeptides at ϳ6.2, 11.4, and 15.8 kDa. Peptide mass mapping and N-terminal amino acid sequencing identified two polypeptides as metabolites of placental transforming growth factor-␤ (11.4 kDa) and bikunin (15.8 kDa). Subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of the anti-human immunodeficiency virus-associated Kaposi's sarcoma active preparations CG-10 (Sigma), Pregnyl (Organon), and Profasi (Serono) revealed the presence of metabolites of placental transforming growth factor-␤ in all three; no other non-human chorionic gonadotrophin-related protein species were observed in these preparations. Our findings present evidence that urinary human chorionic gonadotrophin preparations are contaminated with metabolites of placental transforming growth factor-␤, which may have transforming growth factor-␤ agonist actions, and metabolites of bikunin, which is a protease inhibitor. In combination these molecules may be responsible for the anti-human immunodeficiency virus-associated Kaposi's sarcoma activity demonstrated for these urinary human chorionic gonad-

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Anti-HIV effect of beta subunit of human chorionic gonadotropin (βhCG) in vitro

Cited by 35 publications

References 41 publications

Human chorionic gonadotropin hormone prevents wasting syndrome and death in HIV-1 transgenic mice.

Human chorionic gonadotropin hormone prevents wasting syndrome and death in HIV-1 transgenic mice.

Role of placenta in the vertical transmission of human immunodeficiency virus

Identification of Placental Transforming Growth Factor-β and Bikunin Metabolites as Contaminants of Pharmaceutical Human Chorionic Gonadotrophin Preparations by Proteomic Techniques

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