Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves

Liao, May-Hua; Tsai, Yuan-Nuan; Yang, Chi‐Yu; Juang, Chi-Long; Lee, Ming-Yi; Chang, Li-Hsueh; Wen, Hsiao-Chuan

doi:10.1007/s11418-012-0652-0

Cited by 17 publications

(15 citation statements)

References 23 publications

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“…[ 13 , 14 ], modified by Tseng et al . [ 20 ] and reported in previously paper [ 13 – 15 , 20 ]. Ming-Shueh Shieh, a Professor in Department of Environmental Engineering and Health, who major in identity of plant, preceded the identity of the plant material used in our study prior to its use.…”

Section: Methodsmentioning

confidence: 82%

Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment

Juang

Yang

Hsieh

et al. 2014

BMC Complement Altern Med

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BackgroundSpinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.MethodsThe anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague–Dawley rats weighing 280–320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.ResultsTDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.ConclusionOur studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

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Section: Methodsmentioning

confidence: 82%

Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment

Juang

Yang

Hsieh

et al. 2014

BMC Complement Altern Med

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“…After purification of phytotoxic substance in the extracts of T. diversifolia, a potent phytotoxic substance was isolated and identified as tagitinin C. Tagitinin C has so far been isolated only from T. diversifolia [2,27] and the substance was reported to have antiplasmodial, antiproliferative, antiglioblastoma cytotoxic and insect feeding deterrent activities [10,12,13,15,17]. Tagitinin C was also reported to have growth inhibitory activity against radish, cucumber, tomato and onion [3,23].…”

Section: Growth Inhibitory Activity Of Tagitinin C On Weed Plantsmentioning

confidence: 99%

Phytotoxic property of the invasive plant Tithonia diversifolia and a phytotoxic substance

Suzuki

Iwasaki

Suenaga

et al. 2017

Acta Biologica Hungarica

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Tithonia diversifolia (Hermsl.) A. Gray is a perennial invasive plant and spreads quickly in the invasive areas. The extracts of T. diversifolia were found to be toxic to several crop plant species such as rice, maize, sorghum, lettuce and cowpea, and several putative allelopathic substances were identified. However, there is limited information available for the effects of T. diversifolia on wild plants including weed plant species. We investigated the allelopathic potential of T. diversifolia extracts on weed plants, and searched for phytotoxic substances with allelopathic activity. An aqueous methanol extract of T. diversifolia leaves inhibited the growth of weed plants, Lolium multiflorum Lam., Phleum pretense L., Echinochloa crus-galli (L.) Beauv. The extract was then purified by several chromatographic runs and a phytotoxic substance with allelopathic activity was isolated and identified by spectral analysis as tagitinin C. The substance inhibited the growth of Lolium multiflorum, Phleum pratense and Echinochloa crus-galli at concentrations greater than 0.1 - 0.3 mM. The present results suggest that T. diversifolia may possess allelopathic potential on weed plants and tagitinin C may be responsible for the allelopathic effects of T. diversifolia. The allelopathic potential of T. diversifolia may contribute to its invasive characteristics.

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“…Several biological activities have been described for most of these compounds (Liao et al, , 2013Chagas-Paula et al, 2011, 2012Lin, 2012;Zhao et al, 2012;Sánchez-Mendoza et al, 2011;Gu et al, 2002). The tested STL from T. diversifolia usually exhibit expected biological activity because, in a general way, they are known for their key medicinal potential (Chagas-Paula et al, 2012; Ghantous et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The tested STL from T. diversifolia usually exhibit expected biological activity because, in a general way, they are known for their key medicinal potential (Chagas-Paula et al, 2012; Ghantous et al, 2010). Among all compounds from T. diversifolia already tested for any biological activity, the STL Tag C (1) has been the most frequently studied (Liao et al, , 2013Chagas-Paula et al, 2012;Lin, 2012;Lee et al, 2011;Sánchez-Mendoza et al, 2011;Ghantous et al, 2010;Goffin et al, 2002). In the presence of LPS, all Tags were able to decrease the rate of neutrophil apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…However, in the absence of stimuli an increased rate of apoptosis of neutrophils cultivated with Tag C (1) was observed. Some studies have shown that Tag C (1) induced caspase-dependent apoptosis or even autophagic death of tumor cells, and such role of Tag C (1) is thought to be beneficial for the prevention and treatment of cancer (Liao et al, , 2013Liu et al, 2013). When occurring in the site of infection the exacerbated neutrophil apoptosis would be dangerous because of the late effects in the localized immune response such as modulation or even immunosuppression (Ortega-Gómez et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory sesquiterpene lactones from Tithonia diversifolia trigger different effects on human neutrophils

Abe¹,

Oliveira²,

Dalboni³

et al. 2015

Revista Brasileira de Farmacognosia

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a b s t r a c tThe tagitinins isolated of Tithonia diversifolia (Hemsl.) A. Gray, Asteraceae, are the most studied sesquiterpene lactones due to their wide spectrum of pharmacologic activities, especially related with nuclear factor-kappa B inhibition. Nevertheless, detailed studies about the mechanism of action of its active compounds are still lacking. Neutrophils perform a fundamental role in the inflammatory response to several etiologic factors. However, the effect of tagitinins on human neutrophil is not yet clearly known. We investigated the role of tagitinin C (1), tagitinin F (2) and tagitinin A (3) in activation and survival of human neutrophils to establish possible effects in their mechanisms of inflammation. Human neutrophils were purified from the peripheral blood and cultivated with tagitinins C (1), F (2) and A (3) in the presence or not of Escherichia coli lipopolysaccharide. The enzymatic activity, apoptosis and secretion of cytokines rate were determined after 18 h. Lipopolysaccharide-induced myeloperoxidase activity of human neutrophils was significantly inhibited only by tagitinin F (2). Apoptosis of neutrophils was increased in the presence of tagitinin C (1), and it occurred independently of the presence of lipopolysaccharide or dexamethasone. Tagitinins C (1), F (2) and A (3) decrease lipopolysaccharide-induced interleukin-6, interleukin-8 and Tumor necrosis factor alpha production by human neutrophils. Together, these results indicate that tagitinins exhibit anti-inflammatory action on human neutrophils. However, tagitinin F (2) was the only sesquiterpene lactone that decreased secretion of inflammatory products by neutrophils without inducing neutrophil apoptosis.

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Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves

Cited by 17 publications

References 23 publications

Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment

Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment

Phytotoxic property of the invasive plant Tithonia diversifolia and a phytotoxic substance

Anti-inflammatory sesquiterpene lactones from Tithonia diversifolia trigger different effects on human neutrophils

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