Anti-Hyperglycaemic Effect of Cleome Rutidosperma in Alloxan-Induced Diabetic Albino Rats

Abstract

The hypoglycaemic and antihyperglycaemic effects of methanol extract of leaves of Cleome rutidosperma (Cr) DC (Family: Capparidaceae) was investigated in Wistar rats. Fifty normoglycaemic male rats (120 g-200 g) were divided into groups A (hypoglycaemic study; n=20) and B (antihyperglycaemic study; n=30). Each experiment had one control group and three groups administered with Cr (100, 200 or 400 mg/kg) respectively. Group B had two additional groups of diabetic-untreated rats and glibenclamide-treated diabetic rats. Diabetes was induced in Group B rats (except control) fasted overnight for 12 h by intraperitoneal injection of Alloxan (100 mg/kg). Fasting blood glucose levels (FBGL) were determined and alloxan-treated rats with BGL >200 mg/dl 48 h post-induction were considered diabetic. Data obtained were analyzed using One-way ANOVA and Duncan Multiple Range Test (p<0.05). Cr-treated rats showed significant decline in BGL with noteworthy decline by day 3 post-treatment at the dose of 200 mg/kg (236.40±14.72 mg/dl) from 336.40±21.06 mg/dl. Cr at the dose of 200 mg/kg (72.20±6.18 mg/dl, 69.20±7.81 mg/dl, 137.80±7.15 mg/dl and 70.60±10.66 mg/dl) showed better glycemic control compared to glibenclamide (194.50±7.75 mg/dl, 253.75±7.20 mg/dl, 284.25±10.56 mg/dl and 156.00±10.80 mg/dl). Cr-treated rats also showed progressive weight gain through the course of the study. This study demonstrated Cr has antihyperglycemic effect with more rapid onset of action and better glycemic control compared to glibenclamide.

Review on Bioactive Compounds and Pharmacological Properties of Cleome rutidosperma DC

“…One in vivo study found that administration of aqueous leaf extract of the plant to streptozotocin-diabetic rats enhanced the activity of hepatic glucokinase and inhibited glucose-6-phosphatase (gluconeogenesis enzymatic activity) [31]. The hypoglycaemic and antihyperglycaemic activities of the plant were studied in alloxan diabetic rats [35]. In the model, methanol extract of the plant's leaves at the dose of 200 mg/kg presented stronger glycemic control in comparison with glibenclamide.…”

Ethanol Extract of Crataegus Oxyacantha L. Ameliorate Dietary Non-Alcoholic Fatty Liver Disease in Rat

“…The uses of herbal products have been established for treatment of various type of illness [7] and it also has been long history for 5000 years ago, Iranian traditional medicine has long story and well expressed in Avicenna s book (canon) [8,9]. The plant C. oxyacantha is one the medicinal plants which grow in Mediterranean temperate regions [10], the plant has various traditional application including wound healing and anti-inflammatory effect [10].…”