Anti-hyperglycemic effect of 11-hydroxypalmatine, a palmatine derivative from Stephania glabra tubers

Abstract

A palmatine derivative, named 11-hydroxypalmatine (4), has been isolated from the tubers of Stephania glabra, together with three known alkaloids, palmatine (1), dehydrocorydalmine (2), and stepharanine (3). The structures of the compounds were elucidated by means of spectroscopic analysis including 2D NMR experiments. The hypoglycemic activity of 4 was evaluated against alloxan-induced diabetic mice. The test compound was administered at doses of 25, 50, and 100 mg/kg, p.o., 36 h after alloxan injection (60 mg/kg, i.v.). The alloxan-induced diabetic mice showed significant reduction in blood glucose after treatment with the test compound by 52% as compared to the positive control glibenclamide (54%) and the diabetic control (27%).

Identification of Novel Human Dipeptidyl Peptidase-IV Inhibitors of Natural Origin (Part II): In Silico Prediction in Antidiabetic Extracts

“…We identified 6 molecules isolated from 6 different plants, Aconitum japonicum , Ervatamia officinalis , Solanum nudum , Solanum sodomaeum , Stephania cepharantha and Tabernaemontana eglandulosa (see Table S2), that meet these criteria. The related species with described antidiabetic properties are Aconitum carmichaelii [33], Aconitum moschatum [2], Aconitum violaceum [2], Ervatamia microphylla [55], Solanum lycocarpum [56], Solanum nigrum [57], Solanum xanthocarpum [58], Stephania hernandifolia [59], Stephania glabra [60], Stephania tetrandra [61], and Tabernaemontana divaricata [55]. Therefore, it is plausible to hypothesize that these 6 plants could also have antidiabetic properties mediated, at least partially, by the inhibition of DPP-IV.…”

The genus Stephania (Menispermaceae): Chemical and pharmacological perspectives

“…The test compound was administered at doses of 25, 50, and 100 mg/kg, p.o., 36 h after alloxan injection (60 mg/kg, i.v.). The alloxan-induced diabetic mice showed significant reduction in blood glucose after treatment with the test compound by 52% as compared to the positive control glibenclamide (54%) and the diabetic control (27%) (Semwal et al, 2010b). S. tetrandra S. Moore roots increases the blood insulin level and reduces the blood glucose level in streptozotocin diabetic mice.…”

“…Stephania glabra (Roxb.) Miers (+)-Pronuciferine (33), gindarine, gindaricine, gindarinine, hyndarine, magnoflorine, N-thyloxystephanine, N-methylhydoxystepharine, remerine, stephararine, cycleanine (28), rotundine, capaurine (34), corydalmine (35), stepholidine (36), stepharine (9), protoberberine, palmatine (37), dehydrocorydalmine (38), jatrorrhizine (39), stepharanine (40), columbamine (41), N-desmethycycleanine (42), corynoxidine (43) (Chaudhary and Siddiqui, 1950;Chaudhary et al, 1952;Chopra et al, 1956;Cava et al, 1964Cava et al, , 1968Kin et al, 1965;Robinovich et al, 1965;Shchelchkova et al, 1965;Doskotch et al, 1967;Dhar et al, 1968;Thu and Nuhn, 1971;Khanna et al, 1972;Patra et al, 1980;Bhakuni and Gupta, 1982;Bhakuni, 1984;Mahatma et al, 1987;Anonymous, 1989;Rastogi and Mehrotra, 1991;Duke, 1992;Das et al, 2004), 11-hydroxypalmatine (44) (Semwal et al, 2010b), glabradine (45) and gindarudine (46) (Semwal and Rawat, 2009a,b).…”

Medicinal Formulations of the Kuch Tribe of Bangladesh

“…However, another plant, S. glabra, the roots of which were coadministered with roots of C. trilobata by the Kuch healers for treatment of diabetes, reportedly contains 11-hydroxypalmatine, which demonstrated hypoglycemic activity in alloxan-induced diabetic mice. 20 It is also interesting that the Kuch healers administered a combination of the roots of C. trilobata and S. glabra for 30 days only. During only this time, eating of molasses was forbidden.…”