Anti-hyperglycemic effects of Eryngium billardierei F. Delaroche extract on insulin-resistance HepG2 cells in vitro

Kheirollahzadeh, Fatemeh; Eftekhari, Elahe; Ghollasi, Marzieh; Behzadi, Payam

doi:10.1007/s11033-022-07171-0

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…It is presumed that IL-1 ß is one of the main proinflammatory regulators in association with systemic immunoinflammatory responses. Any dysregulation in the active form of IL-1 ß may result in a wide range of rare autoinflammatory diseases, e.g., Type 2 diabetes [ 126 , 128 , 134 , 140 , 168 , 169 , 191 , 192 ]. Some other autoinflammatory diseases in which the secretion of IL-1 ß gets considerably higher are as follows: chronic infantile neurologic, cutaneous and arthritis (CINCA) syndrome, familial cold autoinflammatory syndrome (FCAS), familial Mediterranean fever (FMF), Muckle-Wells syndrome (MWS), and neonatal-onset multisystem inflammatory disease (NOMID) [ 190 , 193 – 199 ].…”

Section: Methods and Techniques For Determining Cytokinesmentioning

confidence: 99%

The Interleukin-1 (IL-1) Superfamily Cytokines and Their Single Nucleotide Polymorphisms (SNPs)

Behzadi

Sameer

Nissar

et al. 2022

Journal of Immunology Research

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Interleukins (ILs)—which are important members of cytokines—consist of a vast group of molecules, including a wide range of immune mediators that contribute to the immunological responses of many cells and tissues. ILs are immune-glycoproteins, which directly contribute to the growth, activation, adhesion, differentiation, migration, proliferation, and maturation of immune cells; and subsequently, they are involved in the pro and anti-inflammatory responses of the body, by their interaction with a wide range of receptors. Due to the importance of immune system in different organisms, the genes belonging to immune elements, such as ILs, have been studied vigorously. The results of recent investigations showed that the genes pertaining to the immune system undergo progressive evolution with a constant rate. The occurrence of any mutation or polymorphism in IL genes may result in substantial changes in their biology and function and may be associated with a wide range of diseases and disorders. Among these abnormalities, single nucleotide polymorphisms (SNPs) can represent as important disruptive factors. The present review aims at concisely summarizing the current knowledge available on the occurrence, properties, role, and biological consequences of SNPs within the IL-1 family members.

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Section: Methods and Techniques For Determining Cytokinesmentioning

confidence: 99%

The Interleukin-1 (IL-1) Superfamily Cytokines and Their Single Nucleotide Polymorphisms (SNPs)

Behzadi

Sameer

Nissar

et al. 2022

Journal of Immunology Research

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“…HepG2 cell lines have been used as insulin resistance model in several studies. This protocol was adapted from Kheirollahzadeh et al (2022) [16]. To establish an insulinresistant cell model (HepG2/IRM), a gradient of insulin at different concentrations was initially tested for viability through MTT and further glucose quantification was performed.…”

Section: Insulin Resistance Model (Hepg2/irm) and Glucose Uptakementioning

confidence: 99%

Hydroxyhydroquinone and Quassinoids as Promising Compounds with Hypoglycemic Activity through Redox Balance

Santos,

Danetti,

Rastegar

et al. 2024

Compounds

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In the present study, an insulin-resistant cell model (human hepatocellular carcinoma cell line: HepG2) was chosen to investigate the efficacy of two compound classes and their common molecular motif for glycemic control and insulin sensitization. The two compounds’ classes were flavonoid extracts from Rourea cuspidata and quassinoid extracts from Picrasma crenata. The flavonoid-like hydroxyhydroquinone (HHQ) was synthesized. HepG2 cells were tested in a high-glucose environment (HepG2/IRM) by monitoring ROS activity, the concentration of adenosine triphosphate (ATP), and the measurement of mitochondrial membrane potential (MMP). The expression of forkhead box O1 (FOXO1) protein, which mediates gluconeogenesis and insulin resistance, was also investigated using indirect immunocytochemistry and Western blot techniques. A significant increase in glucose uptake and well-regulated ATP concentrations were observed in the treated cells. The downregulation of FOXO1 expression was seen in cells treated with HHQ and quassinoids in comparison to cells treated with flavonoids. This study provides a pharmacological basis for the application of HHQ, quassinoids from P. crenata, and flavonoids from R. cuspidata in the treatment of metabolic diseases such as type 2 diabetes mellitus.

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“…The insulin-resistant HepG2 cell model was established according to the previous study [23] with some minor modifications. Briefly, 2 mL HepG2 cell suspension (~5 × 10 4 cells/mL) was incubated in 6-well plates for 24 h under normal media and conditions.…”

Section: Insulin-resistance Inductionmentioning

confidence: 99%

Polyphenol-Rich Extract of Fermented Chili Pepper Alleviates Insulin Resistance in HepG2 Cells via Regulating INSR, PTP1B, PPAR-γ, and AMPK Pathways

Wang

Cai

et al. 2023

Fermentation

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Fermented Capsicum frutescens L. is a well-known traditional food ingredient in China with a variety of potential nutritional functions due to the increased content of polyphenolic compounds during the fermentation process. This study aimed to investigate the ameliorative effect of fermented chili peppers (FCP) on insulin resistance and the potential mechanism of action. HepG2 cells were treated with 5 × 10−6 mol/L insulin for 12 h to establish the insulin resistance model. The results showed that the ethanol extract of FCP (1 mg/mL), rather than non-FCP extract, significantly increased glucose consumption in insulin-resistant HepG2 cells, which was at least partly attributed to an increase in polyphenolic compounds after fermentation, including kaempferol-3-O-rutinoside, caffeic acid, kaempferol-3-O-glucoside, luteolin, and apigenin. Molecular docking analysis suggested that these five significantly increased polyphenolic compounds in FCP could partially and effectively interact with the key amino acid residues of four key insulin resistance-related receptors (INSR, PTP1B, PPAR-γ, and AMPK). In conclusion, the fermentation process enhanced or even conferred a pronounced anti-insulin resistance effect on chili peppers, and the increased polyphenolic compounds in chili pepper had synergistic effects in modulating the INSR, PTP1B, PPAR-γ, and AMPK pathways to regulate the destruction of glucose consumption.

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Anti-hyperglycemic effects of Eryngium billardierei F. Delaroche extract on insulin-resistance HepG2 cells in vitro

Cited by 11 publications

References 33 publications

The Interleukin-1 (IL-1) Superfamily Cytokines and Their Single Nucleotide Polymorphisms (SNPs)

The Interleukin-1 (IL-1) Superfamily Cytokines and Their Single Nucleotide Polymorphisms (SNPs)

Hydroxyhydroquinone and Quassinoids as Promising Compounds with Hypoglycemic Activity through Redox Balance

Polyphenol-Rich Extract of Fermented Chili Pepper Alleviates Insulin Resistance in HepG2 Cells via Regulating INSR, PTP1B, PPAR-γ, and AMPK Pathways

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