Anti-Hypochlorite and Catalytic Activity of Commercially Available Moringa oleifera Diet Supplement

Abstract

Aiming at the assessment of the pro-health, and especially anti-hypochlorite properties of Moringa oleifera species a representative, commercially available Moringa oleifera dietary supplement was used as a substrate for the preparation of aqueous Moringa extract. The anti-hypochlorite activity of the extract was assessed using the hypochlorite-specific coumarin-based fluorescence turn-off sensor, namely 7-diethylamino-coumarin-3-carboxylic acid (7-DCCA). This compound was synthesized via the Knoevenagel condensation of 4-diethylamino-2-hydroxybenzaldehyde with Meldrum’s acid and the Moringa extract was employed as a medium and catalyst. Moreover, the total phenolic content (TPC) as well as the reactive oxygen species (ROS)–scavenging ability of the aqueous Moringa extract were determined. The results obtained demonstrated the applicability of Moringa extract as an anti-hypochlorite agent. Additionally, the satisfactory yield of the 7-DCCA obtained suggests the usefulness of the extract as a catalyst and the reaction medium. The antioxidative potential of the extract was notably lower than that of the standard (TROLOX). Determination of TPC in 100 g of the dry weight (DW) of studied material revealed a high number of polyphones present.

Structural Features of 1,3,4-Thiadiazole-Derived Ligands and Their Zn(II) and Cu(II) Complexes Which Demonstrate Synergistic Antibacterial Effects with Kanamycin

“…The fully acetylated compound 3 showed the highest IC 50 and compared to 1 and 2 was considered inactive. These findings are consistent with our previous studies [34,35] and with numerous other reports evidencing the polyphenolic moieties as mandatory for high antioxidant potency [36][37][38].…”

Anti-Hypochlorite, Antioxidant, and Catalytic Activity of Three Polyphenol-Rich Super-Foods Investigated with the Use of Coumarin-Based Sensors

“…The reaction yields were referenced to that carried out in 10% of aqueous acetic acid and compared with our previously reported data The use of açaí and schisandra extracts resulted in an identical yield of 7-DCCA obtained (28%), while in the case of the goji extract, it was slightly higher (31%). The reaction yields were referenced to that carried out in 10% of aqueous acetic acid and compared with our previously reported data [19]. All three extracts demonstrated significantly lower catalytic properties compared to that of the dilute acetic acid (56%).…”

“…The mixture was stirred overnight at 20 • C, and the orange solid was then filtered off and recrystallized from methanol, resulting in the formation of crystalline 7-DCCA. The identity and purity of the product isolated were confirmed by HPLC-MS and referenced to our previously obtained data [13,19].…”

“…Synthesis of 7-DCCA probe was carried out according to the previously reported procedure with minor modifications [19]. The powdered plant material (1 g) was suspended in demineralized water (100 mL) and sonicated for 10 min.…”

“…Each plate was shaken for 10 s on the reader shaker, and then the fluorescence measurements started. All samples were excited by light at λ Exc 289 nm [13,19], and the fluorescence emission was recorded within the range of λ 320-700 nm at 25 • C. The results obtained were the average of five exposures of each sample with a beam of light. All experiments were repeated three times.…”