Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma

Gamal‐Eldeen, Amira M.; Raafat, Bassem M.; Alrehaili, Amani A.; El‐Daly, Sherien M.; Hawsawi, Nahed M.; Banjer, Hamsa Jameel; Raafat, Eman; Almehmadi, Mazen

doi:10.3389/fnut.2022.854780

Front. Nutr.

2022

DOI: 10.3389/fnut.2022.854780

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Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma

Amira M. Gamal‐Eldeen

Bassem M. Raafat

Amani A. Alrehaili

et al.

Abstract: Graphical AbstractAnti-hypoxic activity of S. dentifolium through multiple mediators.

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2024

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Enhancing the anticancer effect of metformin through nanoencapsulation: Apoptotic induction, inflammatory reduction, and suppression of cell migration in colorectal cancer cells

Gouhar,

Nasr,

Fahmy

et al. 2024

Archiv der Pharmazie

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Colorectal cancer (CRC) continues to be a significant health challenge, necessitating the development of efficient therapeutic strategies. Drug repurposing, which involves the use of existing medications for new purposes, presents a promising opportunity. Metformin, a widely used antidiabetic drug, has demonstrated potential anticancer effects. To enhance its efficacy, we formulated nano‐metformin, metformin encapsulated within pectin nanoparticles. Our study aimed to evaluate the superiority of nano‐metformin over free metformin in treating CRC. The cytotoxicity of both metformin and nano‐metformin on Caco‐2 CRC cells was assessed using the MTT assay, revealing a significant dose‐dependent inhibition of cell growth using nano‐metformin. The anti‐inflammatory potential was evaluated by measuring the levels of nitric oxide and the pro‐inflammatory cytokines IL‐2 and IL‐6 following lipopolysaccharide (LPS) induction, and the results revealed that treating LPS‐induced cells with nano‐metformin significantly reduced the production of these inflammatory mediators. To elucidate the mechanism of cell death, we employed an acridine orange/ethidium bromide staining assay, which revealed the enhancement of apoptotic cell death following treatment with nano‐metformin. Additionally, we examined the expression of key apoptotic regulators using real‐time qPCR. Nano‐metformin, in particular, significantly downregulated the expression of the antiapoptotic markers Bcl‐2 and Survivin while upregulating the proapoptotic caspases 3, 7, and 9. The comet assay revealed significant DNA damage induced by treatment with the nano‐metformin compared with that in the free form. Moreover, nano‐metformin significantly reduced the migration ability of cells. In conclusion, our work revealed the superior efficacy of our formulated nanoform over free metformin, highlighting its potential as a promising therapeutic agent for CRC treatment.

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Enhancing the anticancer effect of metformin through nanoencapsulation: Apoptotic induction, inflammatory reduction, and suppression of cell migration in colorectal cancer cells

Gouhar,

Nasr,

Fahmy

et al. 2024

Archiv der Pharmazie

View full text Add to dashboard Cite

show abstract

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Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma

Abstract: Graphical AbstractAnti-hypoxic activity of S. dentifolium through multiple mediators.

Cited by 1 publication

References 46 publications

Enhancing the anticancer effect of metformin through nanoencapsulation: Apoptotic induction, inflammatory reduction, and suppression of cell migration in colorectal cancer cells

Enhancing the anticancer effect of metformin through nanoencapsulation: Apoptotic induction, inflammatory reduction, and suppression of cell migration in colorectal cancer cells

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