Anti-Helicobacter pylorieffects of IgY from egg york of immunized hens

Abstract

Effects of egg york containing IgY specific for Helicobacter pylori on the bacterial growth and intragastric infection were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1×10 8 CFU/mL) was incubated with a serially diluted IgY for 3 days. As a result, IgY fully inhibited the bacterial growth at 16 mg/mL, which was determined to a minimal inhibitory concentration. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1×10 8 CFU/mouse) 3 times at 2-day intervals, and 2 weeks later, orally treated twice a day with 50, 100, 200 or 500 mg/kg IgY for 18 days. After the final administration, biopsy sample of the gastric mucosa was assayed for the bacterial identification via urease, oxidase, catalase, nitrate reduction and H 2 S tests in addition to microscopic examination for mucosal inflammation. In CLO kit test, 75, 50, 12.5 and 12.5% of the animals revealed positive reaction following treatment with 50, 100, 200 and 500 mg/kg IgY, respectively, resulting in a superior efficacy at 200 mg/kg than 30 mg/kg pantoprazole that displayed 75% elimination. The CLO test results were confirmed by bacterial identification. Microscopic examination revealed that H. pylori infection caused severe gastric mucosal inflammation, which were not observed in the CLO-negative mice following treatment with IgY or pantoprazole. Taken together, IgY inhibited the growth of H. pylori , and improved gastritis and villi injuries by eliminating the bacteria from the stomach. The results indicate that IgY could be a good candidate overcoming tolerance of antibiotics for the treatment of H. pylori -mediated gastric ulcers.

Comparative analysis of anti-Helicobacter pyloriactivities of FEMY-R7 composed ofLaminaria japonicaandOenothera biennisextracts in mice and humans

“…H. pylori (ATCC49503) was obtained from American Type Culture Collection (Manassas, VA, USA), and cultured on brain heart infusion (BHI) broth in an anaerobic chamber with 10% CO 2 , 5% O 2 , and 85% N 2 at 37℃ with enough humidity [ 35 ].…”

Avian Antibodies (IgY) to Fight Antibiotic Resistance

“…Helicobacter pylori: IgY against H. pylori has been studi ed together with lanzoprazol in human volunteers with good results [13,14].…”

Enterovirus type 71‑immunized chicken egg yolk immunoglobulin has cross antiviral activity against coxsackievirus A16 in�vitro

“…Compared with the IgG from mammals, IgY is more stable (30,31), easy to collect (32), has a high yield (33) and does not react with rheumatoid factors, complement components or mammalian Fc receptors in human serum (34). At present, the application of IgY in the diagnosis and treatment of human diseases has become a research hotspot, particularly regarding infections caused by Helicobacter pylori (35), Vibrio cholerae (36) and other bacterial infections, as well as the infections by human rotavirus (37), severe acute respiratory syndrome coronavirus (38) and other viruses. IgY can also be used for the preparation of diagnostic antibodies for immunohistochemistry, ELISA (39), western blotting and other diagnostic techniques.…”