Anti-Herpes Simplex Virus Activity of Bidens pilosa and Houttuynia cordata

Abstract

The present study evaluated the antiviral activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb., using cytotoxicity test with XTT-based colorimetric assay. BCC-1/KMC cells were infected with herpes simplex virus (HSV) and then were cultured with hot water extract of B. pilosa (HWBP) or H. cordata (HWHC). Results showed that HWBP significantly inhibited the replication of HSV at a concentration of 100 microg/ml (11.9% for HSV-1, p < 0.01; 19.2% for HSV-2, p < 0.005), whereas HWHC had the same effect at a concentration of 250 microg/ml (10.2% for HSV-1, p < 0.05; 32.9% for HSV-2, p < 0.005). The ED50 of HSV type 1 (HSV-1) and HSV type 2 (HSV-2) for HWBP was 655.4 microg/ml and 960 microg/ml respectively, for HWHC it was 822.4 microg/ml and 362.5 microg/ml respectively. Both drugs had selective indexes above 1.04. H. cordata had better effect against HSV-2 than HSV-1, and had a low ED50 against HSV-2. We suggest that H. cordata might be a useful medicinal plant against infection of HSV-2.

Gas chromatography–mass spectrometry analysis of volatile compounds from Houttuynia cordata Thunb after extraction by solid-phase microextraction, flash evaporation and steam distillation

“…It provides a wide range of pharmacological activities including antiviral [7,8], antileukemic [9], antioxidative and antimutagenic effects [10,11]. Medicinal properties claimed for the drug have been attributed to its volatile oil [12,13].…”

Bioactive components and functional properties ofHottuynia cordataand its applications

“…The lowest antiviral concentration needed for each was 4.5, 2.25 and 14 mg/mL, respectively . Chiang et al (2003) evaluated the antiviral activity of H. cordata using a cytotoxicity test with an XTT-based colorimetric assay. BCC-1/ KMC cells were infected with herpes simplex virus (HSV) and then were cultured with a hot water extract of H. cordata (HWHC).…”

“…Since ancient times, H. cordata has been used in China as an edible vegetable and effective traditional Chinese medicine (TCM). It has a wide range of benefits including an antileukemic effect (Kwon et al, 2003), antimutagenic effect , anti-inflammatory action (Chiang et al, 2003), and antianaphylaxis effect , as well as the ability to promote immunologic function. Hayashi et al (1995) reported on its antileukemic activity and virucidal effects on the human immunodeficiency virus (HIV).…”

Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells

“…Hottuynia cordata Thunb is a perennial herb that is native to Southeast Asia (18) and it has a thin stalk and heart-like leaf. It is called E-Sung-Cho in China and it is known to be an effective drug for treating allergic inflammation (19).…”