Anti‐inflammation effects of injectable platelet‐rich fibrin via macrophages and dendritic cells

Abstract

Immune response to implantation materials plays a critical role during early local inflammation and biomaterial-induced regeneration or restoration. A novel platelet concentrate termed i-PRF (injectable platelet-rich fibrin) has recently been developed without any additives by low centrifugation speeds. To date, scientists have investigated the capability of releasing growth factors to improve regeneration but have ignored whether i-PRF can inhibit the inflammatory effect around the wound. The present study investigated the anti-inflammation effects of i-PRF on immune response-related cells, especially macrophages and dendric cells. We found that i-PRF reduced pro-inflammatory M1 phenotype of macrophages and activated dendritic cells around muscle defect that was injected with bacterial suspension. Moreover, in vitro experiments showed similar results. i-PRF deleted inflammatory response caused by lipopolysaccharide to some extent. We determined that TLR4, an activator of inflammatory stimulation and p-p65, a key factor belongs to classical inflammatory related NF-κB signal pathway, can be inhibited by use of i-PRF. Results indicate the potential anti-inflammatory role of i-PRF during regeneration and restoration.

Histological and Histomorphometric Evaluation of Applying a Bioactive Advanced Platelet-Rich Fibrin to a Perforated Schneiderian Membrane in a Maxillary Sinus Elevation Model

“…In contrast, early osteogenesis began to occur under the SM in group A-PRF ( Figures 5A,B). As previously noted, bioactive factors in A-PRF suppress inflammation, and the low-inflammatory microenvironment favored continuous selfrenewal of the sinus cavity in group A-PRF (Nasirzade et al, 2020;Zhang et al, 2020) and reached a dynamic balance between bone formation and resorption. In addition, the significant amount of growth factors and cytokines within A-PRF also played an essential role in regulating the bone remodeling process (Marx, 2004;Aminabadi, 2008;Peerbooms et al, 2010;Soloviev et al, 2014;Herath et al, 2018).…”

Gelatin Nanoparticle‐Injectable Platelet‐Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis

“…In addition, the effects of GNPs‐iPRF on cellular immunity, migration, and the mechanical strength of materials on cell differentiation have not been explored in this study. Among previous studies, it was shown that in addition to promoting angiogenesis, various growth factors within iPRF could also promote cell proliferation, cell migration, and regulate the macrophages to transform to the anti‐inflammation state . It was also proven that mechanical features such as stiffness, stress relaxation, and creep can significantly steer stem cell fate .…”

Injectable Platelet‐Rich Fibrin (PRF): The newest biomaterial and its use in various dermatological conditions in our practice: A case series

“…Along with the platelet growth factors, like that of PRP, 4 this product also documents to contain lymphocytic growth factors and collagen type1. 5 It also produces fibrin network like PRF membrane. 6 Its unique property is that it stays as a liquid for a period of approximately 20 minutes after which the fibrin polymerization takes place and a solid membrane is formed.…”