Anti‐inflammatory action of ark shell (
            <i>Scapharca subcrenata</i>
            ) protein hydrolysate in LPS‐stimulated RAW264.7 murine macrophages

Marasinghe, Chathuri Kaushalya; Jung, Won‐Kyo; Je, Jae‐Young

doi:10.1111/jfbc.14493

Cited by 8 publications

(5 citation statements)

References 62 publications

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“…Apart from their edible value, both ark clams have been found to have beneficial physiological effects. Peptides from the protein hydrolysate of SS and TG were reported to have anti‐inflammatory 17,18 and antioxidant 19,20 effects. Additionally, our previous studies have shown that dietary supplementation with the two ark clams could ameliorate endothelial dysfunction in rats 21 .…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation of ark clams protects gut health and modifies gut microbiota in d‐galactose‐induced aging rats

Tong,

Guo,

et al. 2023

J Sci Food Agric

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BackgroundArk clams, a seafood abundant in various nutrients, are widely consumed worldwide. The present study aimed to investigate the protective benefits of two common ark clams in Korea, Scapharca subcrenata (SS) and Tegillarca granosa (TG), on gut health in D‐galactose (D‐gal)‐induced aging rats.ResultsThirty‐two Wistar rats (11‐week‐old) were randomly allocated into four groups: a CON group (normal diet+saline intraperitoneal (i.p.) injection), a CD group (normal diet+D‐gal i.p. injection), an SS group (normal diet with 5% SS supplementation+D‐gal i.p. injection), and a TG group (normal diet with 5% TG supplementation+D ‐gal i.p. injection). After 12 weeks of treatment, histopathological results showed that gut barrier damage was alleviated in rats of SS and TG groups, as evidenced by increases in mucus layer thickness and goblet cell numbers. Meanwhile, the two groups supplemented with ark clams showed an evident reduction in oxidative stress biomarkers (MDA and PCOs levels in the colon) and an increase in the immune‐related factor (IgA level in the plasma) in rats. The 16S rRNA analysis revealed that SS and TG ark clams significantly increased the proliferations of Bacteroidetes at the phylum level and Parabacteroides at the genus level. Additionally, the levels of the three main short‐chain fatty acids (SCFAs) in the cecal contents were also significantly increased in the SS and TG groups.ConclusionOur results indicated a potent preventive effect of SS and TG ark clams on D‐gal‐induced gut injury, suggesting that ark clams may be a promising dietary component for intervening in aging.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

Dietary supplementation of ark clams protects gut health and modifies gut microbiota in d‐galactose‐induced aging rats

Tong,

Guo,

et al. 2023

J Sci Food Agric

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“…The levels of p-NF-κB protein in LPS-induced cells fell by 13.5%, 48.0%, and 52.0% when treated with 5, 10, and 20 g/mL of FLs, respectively. Blocking the activation of extracellular signal-regulated kinase (ERK) and P38 in the MAPK signaling pathway is another effective means to suppress inflammation [37,38]. Compared to the control group, the protein levels of p-P38 and p-ERK in the LPS group were enhanced 22.9-fold and 18.3-fold, respectively (Figure 3A).…”

Section: Effects Of Fls On Blocking Nf-κb/mapk Signaling Pathwaymentioning

confidence: 99%

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

Jing

Huang³

et al. 2023

Foods

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Flaxseed linusorbs (FLs), cyclic peptides derived from flaxseed oils, have shown multiple activities such as anticancer, antibacterial, and anti-inflammatory effects. However, the anti-inflammatory monomers of FLs and their mechanisms are still unclear. In this study, we have elucidated that FLs suppress the modulation of NF-κB/MAPK signaling pathways by targeting the inhibition of activating TLR4 in LPS-induced RAW 264.7 cells. Therefore, the transcription and expression of inflammatory cytokines (i.e., TNF-α, IL-1β, and IL-6) and inflammatory mediator proteins (i.e., iNos and Cox-2) were significantly suppressed by FLs. In addition, an in silico study discovered that eight monomers of FLs showed high-affinity bindings with TLR4. In silico data combined with HPLC results indicated that FLA and FLE, accounting for 44%, were likely the major anti-inflammatory monomers in FLs. In summary, FLA and FLE were proposed as the main anti-inflammatory active cyclopeptides via hindering TLR4/NF-κB/MAPK signaling pathways, suggesting the potential use of food-derived FLs as natural anti-inflammatory supplements in a daily diet.

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“…Weight Distribution and Amino Acid Composition. Te molecular weight distribution and amino acid composition of ASPH were determined as described by Marasinghe et al [30]. Molecular weight distribution was analyzed using the HPLC system (Dionex UltiMateTM 3000, Termo Scientifc) equipped with a Superdex Peptide 10/300 GL column (No.…”

Section: Characterization Of Asph On Molecularmentioning

confidence: 99%

“…Association of Ofcial Analytical Chemist (AOAC) standard protocols were followed to analyze the proximate composition of ASPH according to the method of Marasinghe et al [30]. Briefy, the method 988.12 (phenol-sulfuric acid method), method 922.06, method 923.03, and method 925.10 were used to determine crude carbohydrate content, crude lipid, crude ash, and moisture content, respectively.…”

Section: Determination Of Proximate Composition Of Asphmentioning

confidence: 99%

OxLDL-Induced Foam Cell Formation Inhibitory Activity of Pepsin Hydrolysate of Ark Shell (Scapharca subcrenata (Lischke, 1869)) in RAW264.7 Macrophages

Marasinghe

Jung

2023

Journal of Food Biochemistry

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Inhibitory effect of ark shell (Scapharca subcrenata (Lischke, 1869)) proteolytic hydrolysates (ASHs) on oxidized low-density lipoprotein (oxLDL)-induced macrophage foam cell formation was investigated. Two types of ASHs were prepared by Alcalase® and pepsin, ASAH (ark shell-Alcalase® hydrolysates), and ASPH (ark shell-pepsin hydrolysate). Oil Red O staining results showed that ASPH suppressed foam cell formation and lipid accumulation more than ASAH in oxLDL-induced foam cell formation of RAW264.7 macrophages. ASPH reduced the levels of total cholesterol, cholesterol ester, and free cholesterol in oxLDL-treated RAW264.7 macrophages. It was found that ASPH increased cholesterol efflux and decreased cholesterol influx rate. In this regard, protein expressions of CD36 and scavenger receptor class A1 (SR-A1) for cholesterol influx and ATP-binding cassette transporter A1 and G1 (ABCA1 and ABCG1) for cholesterol efflux were investigated. ASPH treatment resulted in an increase of ABCA1 and ABCG1 expression but downregulated CD36 and SR-A1 expression. Furthermore, ASPH suppressed production of proinflammatory cytokines, including tumor necrosis factor-α and interleukin-6 and -1β, through regulating nuclear factor-kappa B (NF-κB) in oxLDL-induced foam cell formation of RAW264.7 macrophages. Taken together, our data indicate that ASPH might be a useful ingredient in functional foods for ameliorating atherosclerosis by preventing foam cell formation.

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Anti‐inflammatory action of ark shell ( Scapharca subcrenata ) protein hydrolysate in LPS‐stimulated RAW264.7 murine macrophages

Cited by 8 publications

References 62 publications

Dietary supplementation of ark clams protects gut health and modifies gut microbiota in d‐galactose‐induced aging rats

Dietary supplementation of ark clams protects gut health and modifies gut microbiota in d‐galactose‐induced aging rats

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

OxLDL-Induced Foam Cell Formation Inhibitory Activity of Pepsin Hydrolysate of Ark Shell (Scapharca subcrenata (Lischke, 1869)) in RAW264.7 Macrophages

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