Anti-inflammatory action of N-stearoylethanolamine in rat peritoneal macrophages during high fat feeding

Onopchenko, Oleksandra; Kosiakova, H. V.; Berdyshev, A. G.; Hula, N M

doi:10.1016/j.atherosclerosis.2018.06.598

Cited by 1 publication

(2 citation statements)

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“…Control rats were further subdivided into control (n = 10) and NSE (n = 7) groups. The size of NSE group (control rats + NSE) was reasonably reduced according to previous data reported no physiological action of NSE in the case of healthy control animals (Onopchenko et al, 2018).…”

Section: Animal Modelmentioning

confidence: 51%

“…NSE was also shown to prevent Fe 2+ -dependent oxidation of lipids induced by free radicals in cardiac and liver mitochondria (Parinardi et al, 1988) (Gulaya et al, 1998). In contrast to other NAEs, NSE does not interact with cannabinoid receptors (Movahed et al, 2005) and its action may be realized via rearranging membrane lipids and modulation of proinflammatory signals (Onopchenko et al, 2018). However, the exact mechanisms of the action of NSE remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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N-Stearoylethanolamine Inhibits Integrin-Mediated Activation, Aggregation, and Adhesion of Human Platelets

Hudz

Chernyshenko

Kasatkina

et al. 2022

J Pharmacol Exp Ther

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N-stearoylethanolamine (NSE), a lipid mediator that belongs to the N-acylethanolamine (NAE) family, has anti-inflammatory, antioxidant and membranoprotective actions. In contrast to other NAEs, NSE does not interact with cannabinoid receptors. The exact mechanism of its action remains unclear. The aim of this study was to evaluate the action of NSE on activation, aggregation and adhesion of platelets that were chosen as a model of cellular response.Aggregation of platelets was measured to analyze the action of NSE (10 -6 -10 -10 M) on platelet reactivity. Changes in granularity and shape of resting platelets and platelets stimulated with ADP in the presence of NSE were monitored by flow-cytometry, platelet deganulation was monitored by spectrofluorimetry. In vivo studies were performed using obese insulin-resistant rats. Binding of fibrinogen to the GPIIb/IIIa receptor was estimated using indirect ELISA and scanning electron microscopy (SEM). It was found that NSE inhibits the activation and aggregation of human platelets. Our results suggest that NSE may decrease the activation and subsequent aggregation of platelets induced by ristocetin, epinephrine and low doses of ADP.NSE also reduced the binding of fibrinogen to GPIIb/IIIa on activated platelets. These effects could be explained by the inhibition of platelet activation mediated by integrin receptors: the GPIb-IX-V complex for ristocetin-induced activation and GPIIb/IIIa when epinephrine and low doses of ADP were applied. The anti-platelet effect of NSE complements its anti-inflammatory effect and allows us to prioritize studies of NSE as a potent anti-thrombotic agent. Significance StatementN-stearoylethanolamine (NSE) was shown to possess inhibitory action on platelet activation, adhesion and aggregation. The mechanism of inhibition possibly involves integrin receptors. This finding complements the known anti-inflammatory effects of NSE.

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Section: Animal Modelmentioning

confidence: 51%