Anti-inflammatory action of resveratrol in the central nervous system in relation to glucose concentration - an in vitro study on a blood-brain barrier model

Abstract

Objective and Design: This study aimed to investigate the anti-inflammatory properties of resveratrol (RSV) on astrocyte cytokine profiles within the in vitro model of blood-brain barrier (BBB) under varying glucose concentrations (2.2, 5.0, and 25.0 mmol/L). Material:The subjects comprised the in vitro co-cultures of endothelial cells and astrocytes within the BBB model with 0.4-µm-wide pores between them. Treatment:RSV was administered at a dosage of 50 μM to the microvascular compartment (MC), and the duration of the intervention was 24 hours. Methods: Cytokine levels (IL-1 α, IL-1 β, IL-2, IL-4, IL-6, IL-8) in the brain compartment (BC) were assessed using a Multi-Analyte ELISArray Kit before and after the addition of LPS and RSV. Statistical analysis was conducted to determine significance levels. Results: RSV significantly reduced the concentration of all studied cytokines in the BC, regardless of glucose levels. The most substantial decrease occurred under normoglycemic conditions. The concentration of RSV in the BC was highest in normoglycemic conditions compared to hypo- and hyperglycemia. Conclusions:Administration of RSV in the MC demonstrated anti-inflammatory effects within BC, particularly in normoglycemia-simulating conditions. Future in vivostudies, including animal and human research, are needed to explore the bioavailability of RSV within the CNS.