Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

Gdula‐Argasińska, Joanna; Paśko, Paweł; Sułkowska-Ziaja, Katarzyna; Kała, Katarzyna; Muszyńska, Bożena

doi:10.18388/abp.2017_1534

Cited by 10 publications

(7 citation statements)

References 43 publications

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“…There exist many pharmacological reports on the compounds that have been isolated from this plant. For instance, Linolenic acid (1) has been reported to have medicinal properties such as anti-inflammatory (Gdula-Argasińska et al, 2017). Though there are no reported pharmacological activities reported for 1methyl cyclobutene (2), related compounds with cyclobutane ring such as tripartilactum from Streptomyces species have been reported to act as N + /K + ATPase inhibitor (Park et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Bioactive secondary metabolites from the leaves of <I>Secamone africana</I> (Olive.) Bullock

Sekandi¹,

Namukobe²,

Boyer³

et al. 2020

Int. J. Bio. Chem. Sci

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Secamone africana leaves are used in the treatment of malaria and other ailments in Uganda. The aim of the study was to characterize the antiplasmodial compounds from the leaves of Secamone africana. The leaves were extracted sequentially using dichloromethane (DCM) and methanol (MeOH). The crude extracts and isolated compounds were evaluated for their antiplasmodial activity against the chloroquine sensitive Sierraleone I (D6) and chloroquine-resistant Indochina I (W2) strains of Plasmodium falciparum. Isolation and purification were done using chromatographic techniques including column chromatography and high performance liquid chromatography. The isolated compounds were characterized using spectroscopic methods. The MeOH extract (IC50 = 5.45 μg/mL) was found to be more active than the DCM extract (IC50= 15.93 μg/mL) against the D6 malaria parasite. Chemical investigation of the MeOH extract yielded one new compound; 2-(2,4-dimethyloxetan-2-yl) acetic acid (3) in addition to the six known compounds; α-linolenic acid (1), conduritol B (4), β-sitosterol (5), 3,4-dihydroxybenzoic acid (6), 4-hydroxybenzoic acid (7) and coumaric acid (8). The DCM extract yielded one known compound: 1-methyl cyclobutene (2). The presence of these compounds with good anti-plasmodial activities and other bioactivities reported in literature, appears to argue for the therapeutic potential of Secamone africana.Keywords: Secamone africana,, anti-plasmodial activity, chromatography, secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

Bioactive secondary metabolites from the leaves of <I>Secamone africana</I> (Olive.) Bullock

Sekandi¹,

Namukobe²,

Boyer³

et al. 2020

Int. J. Bio. Chem. Sci

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“…KLF2 also mediates acute and chronic inflammations [17, 18]. The anti-inflammatory effects of KLF2 mechanistically are linked to the suppression of nuclear factor-kappa B (NF- κ B) signaling [17, 19] that regulates a variety of genes related to inflammatory responses [20, 21]. Thus, KLF2 may be a key thrombotic regulator due to its effects on regulating both endothelial function and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation

Wang

Gao

et al. 2019

Oxidative Medicine and Cellular Longevity

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The Yiqi-Huoxue granule (YQHX) is a traditional Chinese medication widely used in the therapy of the traditional Chinese medicine diagnosis “Qi deficiency” or “blood stasis” in China. Both these symptoms are related to inflammation, but the mechanisms of YQHX against inflammation are largely unknown. Thus, our present study investigated the effects of YQHX on regulating inflammatory responses induced by lipopolysaccharides (LPS) in HUVECs. Our data found that YQHX remarkably inhibits the production of prothrombotic factors, plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF), while it upregulates the protein expression of Kruppel-like factor 2 (KLF2). The increase in PAI-1 and TF was significantly attenuated through a transgenic knockdown in KLF2 with a Lenti-shKLF2 vector. YQHX also decreases the phosphorylation of nuclear factor-κB (NF-κB) p65 and IκB following LPS stimulation, and it effectively suppresses PAI-1 and TF via a NF-κB-dependent mechanism. Taken together, our results suggest that YQHX provides a notable antithrombotic activity via regulating the KLF2 expression and NF-κB signaling pathway in HUVECs. The KLF2 and NF-κB may be potential therapeutic targets for interventions of inflammation associated with atherosclerosis.

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“…28 The presence of tryptophan in C has also been previously found in the extracts of garlic sprouts. 29 For organosulfur compounds, a marginal significance of allyl mercaptan is found in S+F, while its precursor, allicin, is relevant in C with high significance. This is attributed to the degradation of alliin (S-allyl-L-cysteine sulfoxide) to allicin, 24 whose mechanism can be triggered when the garlic clove is cut and exposed to air.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

HR-μMAS NMR-Based Metabolomics: Localized Metabolic Profiling of a Garlic Clove with μg Tissues

et al. 2018

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The localization of metabolic profiles within a tissue sample is of particular interest when the sampling size is considerably small, i.e. in the order of microgram (µg) scale. Small sampling size is inevitable when the sample availability is limited, or when different metabolic profiles are suspected in small disparate sample regions. Capitalizing a recently introduced high-resolution micro-MAS probe (HR-µMAS) for its capability of high-quality NMR data acquisition of µg samples, this study explores the localized metabolic NMR profiling of a single garlic clove and compares the methodology and results with the standard HR-MAS. One advantage of HR-µMAS is the feasibility of analyzing homogenous µg samples within a large heterogeneous tissue. As a result, the sampling mass (< 0.5 mg) allows to selectively profile four homogenous anatomic garlic regions by HR-µMAS (skin, flesh, inner epidermis and sprout), in contrast to three regions (skin, flesh and core ≡ inner epidermis & sprout) by HR-MAS with a sampling mass of ca. 8 mg. Discriminant analysis was carried out to identify the significant variables in the different regions. It found a significant presence of fructose in both skin and flesh, while sucrose and glucose are predominant in the core. Among the garlic characteristic sulfur compounds, allicin is dominant in the core and allyl mercaptan in both skin and flesh. Quantification analysis was conducted and demonstrated its potential by quantifying metabolites at µg-level. This study offers an important basis for designing HR-µMAS NMR-based metabolomics studies that can benefit from profiling µg-scale samples.

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Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

Cited by 10 publications

References 43 publications

Bioactive secondary metabolites from the leaves of <I>Secamone africana</I> (Olive.) Bullock

Bioactive secondary metabolites from the leaves of <I>Secamone africana</I> (Olive.) Bullock

Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation

HR-μMAS NMR-Based Metabolomics: Localized Metabolic Profiling of a Garlic Clove with μg Tissues

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