Anti-inflammatory activities of the products of supercritical fluid extraction from Litsea japonica fruit in RAW 264.7 cells

Song, Sang-Mok; Ham, Young-Min; Ko, Yoeng-Jong; Ko, Eun-Yi; Oh, Dae-Ju; Kim, Chang-Sook; Kim, Daekyung; Kim, Kil-Nam; Yoon, Weon‐Jong

doi:10.1016/j.jff.2016.01.008

Cited by 17 publications

(15 citation statements)

References 42 publications

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“…During the inflammatory process, significant amounts of NO and prostaglandin E2 are produced via the expression of iNOS and COX-2 (Lu et al 2017). The excessive production of proinflammatory mediators by macrophages can induce variety of inflammation-related disorders; thus, agents that suppress the production of these factors may have the potential to protect against inflammatory diseases (Jung et al 2007;Yun et al 2014;Song et al 2016). We investigated the inhibitory effects of diosmin on LPS-induced proinflammatory media-tors in RAW264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

“…NF-κB, a primary transcription factor, regulates the expression of various cellular gene-encoding factors for inflammatory responses. The MAPK signaling pathways, which include extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, are known to induce COX-2 and iNOS expression in LPS-stimulated macrophage (Song et al 2016). Moreover, Guha and Mackman (2001) have reported that MAPKs play a critical role in the activation of the NF-κB signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Guha and Mackman (2001) have reported that MAPKs play a critical role in the activation of the NF-κB signaling pathway. Therefore, compounds that regulate both the NF-κB and MAPKs pathways have the potential to prevent inflammatory diseases (Song et al 2016). Diosmin (3ʹ,5,7-trihydroxy-4ʹ-methoxyflavone 7-rutinoside) is an unsaturated flavonoid glycoside, presents in citrus fruits (Melin et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Berköz

2019

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Diosmin is an unsaturated flavonoid glycoside, presents in citrus fruits. The aim of this study is to investigate the molecular mechanism of diosmin with respect to the NF-κB and MAPKs signaling pathways. Firstly, 10, 20, 30, 40 and 50 µM diosmin were treated to lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The anti-inflammatory effects of diosmin was displayed via measuring prostaglandin E 2 (PGE 2), nitric oxide (NO), interleukines (IL-6, IL-12), tumor necrosis factor α (TNF-α) production, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), IL-6, IL-12, TNF-α mRNA levels, and phosphorylation levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκB-α) and mitogen-activated protein kinases (MAPKs); JNK, ERK, and p38 in LPS induced RAW264.7 macrophages. Our study showed that especially high concentrations of diosmin decreased NO, PGE 2 , IL-6, IL-12, TNF-α production and mRNA levels of these mediators (p < 0.05). The expression of phosphorylated-JNK was significantly suppressed by diosmin at 40 and 50 µM concentrations. Furthermore, diosmin significantly inhibited the expression of phosphorylated-ERK, p38, and p-IκB-α in a dose-dependent manner. Our results suggest that diosmin is a potent anti-inflammatory agent and has potential for development into a therapeutic agent for inflammation-associated disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Berköz

2019

gpb

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“…NF- κ B, a primary transcription factor, regulates the expression of various cellular gene-encoding factors for inflammatory responses. The MAPK signaling pathways, which include extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, are known to induce COX-2 and iNOS expression in LPS-stimulated macrophage [ 5 ]. Moreover, Guha and Mackman [ 6 ] have reported that MAPKs play a critical role in the activation of the NF- κ B signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Anti‐Inflammatory Effects of Flavonoid‐Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide‐Stimulated RAW 264.7 and Peritoneal Macrophages

Nam

Lim

Lee

et al. 2017

Oxidative Medicine and Cellular Longevity

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Buckwheat sprouts have been widely consumed all around world due to their great abundance of bioactive compounds. In this study, the anti-inflammatory effects of flavonoid-rich common buckwheat sprout (CBS) and tartary buckwheat sprout (TBS) extracts were evaluated in lipopolysaccharide- (LPS-) stimulated RAW 264.7 murine macrophages and primary peritoneal macrophages from male BALB/c mice. Based on the reversed-phase HPLC analysis, the major flavonoids in CBS were determined to be C-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin), quercetin-3-O-robinobioside, and rutin, whereas TBS contained only high amounts of rutin. The TBS extract exhibited higher inhibitory activity as assessed by the production of proinflammatory mediators such as nitric oxide and cytokines including tumor necrosis factor-α, interleukin- (IL-) 6, and IL-12 in LPS-stimulated RAW 264.7 macrophages than CBS extract. In addition, TBS extract suppressed nuclear factor-kappa B activation by preventing inhibitor kappa B-alpha degradation and mitogen-activated protein kinase phosphorylation in LPS-stimulated RAW 264.7 macrophages. Moreover, the TBS extract markedly reduced LPS-induced cytokine production in peritoneal macrophages. Taken together, these findings suggest that TBS extract can be a potential source of anti-inflammatory agents that may influence macrophage-mediated inflammatory disorders.

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“…The qualities of Litsea (genus of the Laurel family, Lauraceae), mostly in the form of the ethanolic (EtOH) or hexane fragmentations of the plant, as an antioxidant agent with anti-inflammatory characteristics, have been evaluated, inter alia, within the setting of OA [52][53][54][55][56][57][58]. Data reported from in vitro research, investigating the anti-inflammatory assets of a variety of fractions (EtOH or other) of this plant within the context of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, suggest a considerable inhibitory activity of the intervention on modulators of inflammation (NO and PGE 2 , iNOS, and COX-2), expression levels, along with pro-inflammatory cytokines, IL-1β, IL-6, TNF-α, and NF-κB (p65 and p50) activation, and mitogen-activated protein kinase (MAPK) phosphorylation [52][53][54].…”

Section: Litsea Japonicamentioning

confidence: 99%

Unraveling Natural Products’ Role in Osteoarthritis Management—An Overview

et al. 2020

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The natural process of aging gradually causes changes in living organisms, leading to the deterioration of organs, tissues, and cells. In the case of osteoarthritis (OA), the degradation of cartilage is a result of both mechanical stress and biochemical factors. Natural products have already been evaluated for their potential role in the prevention and treatment of OA, providing a safe and effective adjunctive therapeutic approach. This review aimed to assess the therapeutic potential of natural products and their derivatives in osteoarthritis via a systematic search of literature after 2008, including in vitro, in vivo, ex vivo, and animal models, along with clinical trials and meta-analysis. Overall, 170 papers were obtained and screened. Here, we presented findings referring to the preventative and therapeutic potential of 17 natural products and 14 naturally occurring compounds, underlining, when available, the mechanisms implicated. The nature of OA calls to initially focus on the management of symptoms, and, in that context, several naturally occurring compounds have been utilized. Underlying a global need for more sustainable natural sources for treatment, the evidence supporting their chondroprotective potential is still building up. However, arriving at that kind of solution requires more clinical research, targeting the implications of long-term treatment, adverse effects, and epigenetic implications.

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Anti-inflammatory activities of the products of supercritical fluid extraction from Litsea japonica fruit in RAW 264.7 cells

Cited by 17 publications

References 42 publications

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Comparison of Anti‐Inflammatory Effects of Flavonoid‐Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide‐Stimulated RAW 264.7 and Peritoneal Macrophages

Unraveling Natural Products’ Role in Osteoarthritis Management—An Overview

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