Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

Abstract

The objective of this study was to evaluate the usefulness of a hydroalcoholic extract from Galinsoga parviflora herb (GP) in some aspects of the endothelial cell function necessary for anti-inflammatory activity and wound healing and relate these to the GP phytochemical profile. This study demonstrated that the GP extract caused a dose-dependent reduction of IL-6 secretion on IL-1β-stimulated endothelial cells. The IL-6 release was decreased to 33% ± 9% while this did not influence the IL-6 secretion without stimulation. Additionally, the GP extract exhibited an anti-hyaluronidase activity (IC50 = 0.47 mg/mL), which was evidently stronger than the positive control kaempferol (IC50 = 0.78 mg/mL) as well as a moderate and concentration-dependent, antioxidant activity. The results of the scratch assay showed that exposure of the endothelial cells to GP induced complete healing of the damage after 12 h of the study. The phytochemical profile of the extract was studied by using spectrophotometric (total amount of polyphenols and flavonoids) and UPLC (phenolic acids) methods. The main compound in the GP extract was a chlorogenic acid (2.00 ± 0.01 mg/g by UPLC). The total content of polyphenols was 98.30 ± 0.14 mg of chlorogenic acid equivalent/g of the dry herb and content of flavonoids amounted to 6.15 ± 0.41 mg quercetin equivalent/g of the dry herb. Moreover, the presence of flavonoids in G. parviflora was provided after their isolation and identification by spectroscopic methods. In conclusion, it demonstrated that application of GP in the treatment of skin lesions gives possibility of wound healing based on antioxidant, anti-inflammatory, and hyaluronidase-inhibiting activities of G. parviflora herb extract.

Permeability of Hypogymnia physodes Extract Component—Physodic Acid through the Blood–Brain Barrier as an Important Argument for Its Anticancer and Neuroprotective Activity within the Central Nervous System

“…TPC was determined using the Folin–Ciocalteu method [ 35 ]. In total, 0.1 mL of the extract, dissolved in DMSO at concentration 2 mg/mL, was mixed with 4.0 mL of distilled water and with 0.5 mL of Folin–Ciocalteu reagent.…”

Ethnopharmacological Survey on Treatment of Hypertension by Traditional Healers in Bukavu City, DR Congo

“…Fresh leaves and juice of G. parviflora have been used in folk medicine worldwide to treat dermatological disorders, including eczema, lichen, and nonhealed bleeding wounds. e use of G. parviflora as food by humans for making salad and soups in Latin and North America proves that the plant is nontoxic [34]. Dissotis trothae extracts have antidiarrhoeal action [35], and the leaves are used across Africa without strong scientific basis or safety concerns.…”

Role of graphene oxide/yttrium oxide nanocomposites as a cathode material for natural dye‐sensitized solar cell applications

“…The band gap energies for GO GO/Y 2 O 3 (5:1, 5:2 and 5:3) nanocomposites, JG dye are found to be 3.2 eV,4.5 eV, 4.3 eV, 3.9 eV and 4.2 eV respectively. [17][18][19][20] It is observed from Figure 9a,b that the band gap decreases due to the presence of Y 2 O 3 nanoparticles concentration onto the surface of GO surface. This blue shift attributes to the increase in crystallite size of the GO/Y 2 O 3 (5:1, 5:2, and 5:3) nanoparticles and could also be evidenced from XRD analysis.…”