Anti-inflammatory activity in vitro, extractive process and HPLC-MS characterization of total saponins extract from Tribulus terrestris L. fruits

“…The method for determining the amount of NO produced by macrophages has been described in previous studies [8,9]. Briefly, RAW 264.7 cells (1 × 10 5 cells/ml) were cultured in a 96-well plate for 2 h. Then, SHL extracts (200 μg/ml) from different harvest time and DEX (10 μM) were added to the cell and stimulated with LPS (1 μg/ ml) at 37 °C for 18 h. Finally, the mixtures of the Griess reagent and cell culture supernatants were measured at 550 nm using a Tecan infinite ® F200 microplate reader (Tecan Group, Ltd., Männedorf, Switzerland).…”

Section: Determination Of No Production and Cell Viabilitymentioning

confidence: 99%

Discrimination and quality evaluation of fifteen components in Stauntonia hexaphylla leaves from different harvest time by HPLC–PDA–ESI–MS/MS and ELSD coupled with multivariate statistical analysis and anti-inflammatory activity evaluation

et al. 2020

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The leaves of Stauntonia hexaphylla (SHL) are a very popular herbal medicine in Korea because it can be used to treat rheumatic osteoporosis and other diseases. However, owing to the inconsistency in harvesting time and growth years, their quality is uneven, which indirectly negatively affects the safety of this medication. Therefore, the difference of constituents in SHL harvested at different seasons and years were analyzed by high-performance liquid chromatography coupled to photodiode-array and electrospray ionization mass spectrometry detectors (HPLC–PDA–ESI/MS). A total of 15 components were tentatively characterized in samples of SHL, including 5 compounds reported for the first time in this plant. Moreover, the relative content of these constitutions was simultaneously determined by HPLC coupled with evaporative light scattering detection (ELSD). Hierarchical clustering analysis (HCA) and principal component analysis (PCA) revealed that the quality has a certain extent difference in different harvest times, the best harvest time was 3 years old growing in autumn. The same harvest time was also suggested based on the anti-inflammatory evaluation.

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“…The isolation and structure determination of five steroidal saponins present in Agave sisalana has been reported to confirm the basis of chemical and phytochemical affirmation that this plant can be used in pharmacological tests [ 12 ]. There are numerous pharmacological treatments derived from saponins, such as antifungal, anti-allergic, anti-inflammatory, antibiotic, hepatoprotective, and antitumor activity [ 8 , 13 , 14 , 15 , 16 ]. Moreover, steroidal sapogenins, lipophilic portions of saponins, are the precursors of pharmacologically active steroid anti-inflammatory drugs present in glucocorticoids [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity and Anti-Inflammatory Effects of Agave sisalana Extract Derived from Agroindustrial Residue

Costa

Fracasso

Guarnier

et al. 2023

Plants

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Background: In several countries, the leaf juice of Agave sisalana (also known as sisal) is widely used topically, especially as an antiseptic, and orally for the treatment of different pathologies. However, in Brazil, which is the largest producer of Agave sisalana, its residue, which represents the majority of its weight, has been thrown away. For this reason, the determination of the pharmacological and toxicological potentials of sisal residue and its possible therapeutic use is seen as a way to contribute to the sustainable development and social promotion of the largest producer of sisal in Brazil, the interior of Bahia State, which is among the poorest areas in the country. Given the scarcity of available scientific studies on the pharmacological and toxicological properties of sisal residue juice, this study aimed to promote the acid hydrolysis of this juice to potentiate the anti-inflammatory effect already described in the literature. Furthermore, it aimed to evaluate the toxicological profile of the hydrolyzed extract (EAH) and to determine its acute toxicity, as well as its side effects on the reproductive aspects of rats. Method: The anti-inflammatory effect of EAH was evaluated in vitro using the induction of hemolysis by hypotonic solution and in vivo in rats using the carrageenan-induced paw edema test and the xylene-induced ear edema test. The acute toxicity, resulting from a single-dose administration, was investigated for some manifestation of toxic symptoms related to motor control and consciousness in rats. At a concentration of 100 mg/kg, by repeated doses, the reproductive toxicity effects of EAH in rats were assessed. Results: In vitro anti-inflammatory activity was positive using the human red blood cell membrane stabilization method. In both in vivo tests used to assess the anti-inflammatory activity, EAH (at three doses) significantly inhibited edema when compared to the control group. At a dose of 50 mg/kg, EAH exhibited a greater effect than indomethacin, a nonsteroidal anti-inflammatory drug with known activity. In vivo toxicological studies have shown that EAH does not present toxic effects when administered orally in a single dose, up to 1000 mg/kg. Finally, EAH promoted a gonadotoxic effect and increased the embryonic mortality rate after implantation. Conclusions: It is suggested that the anti-edematogenic effect of the acid hydrolysis extract from sisal juice is due to the high concentration of steroidal sapogenins. Therefore, this extract can be considered a potential new anti-inflammatory or even an important sapogenin source for the development of steroidal glucocorticoids. However, further studies are needed to elucidate the chemical composition of sisal juice. Regarding toxicology studies, EAH did not show cytotoxic and clastogenic potentials, but it presented a powerful reproductive toxic effect in rats.

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Anti-inflammatory activity in vitro, extractive process and HPLC-MS characterization of total saponins extract from Tribulus terrestris L. fruits

Cited by 25 publications

References 37 publications

Role of natural products towards the SARS-CoV-2: A critical review

Role of natural products towards the SARS-CoV-2: A critical review

Discrimination and quality evaluation of fifteen components in Stauntonia hexaphylla leaves from different harvest time by HPLC–PDA–ESI–MS/MS and ELSD coupled with multivariate statistical analysis and anti-inflammatory activity evaluation

Toxicity and Anti-Inflammatory Effects of Agave sisalana Extract Derived from Agroindustrial Residue

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