Anti-inflammatory activity of a fixed combination of probiotics and herbal extract in an in-vitro model of intestinal inflammation by stimulating Caco-2 cells with LPS-conditioned THP-1 cells medium

Borgonetti, Vittoria; Cocetta, Veronica; Biagi, Marco; Carnevali, Ilaria; Governa, Paolo; Montopoli, Monica

doi:10.23736/s2724-5276.20.05765-5

Cited by 6 publications

(8 citation statements)

References 42 publications

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“…Caco-2 is an immortalised colon cancer cell line derived from human colorectal adenocarcinoma cells [ 227 ]. The differentiated Caco-2 cells exhibit features that closely resemble small bowel enterocytes, including brush-border microvilli [ 228 , 229 ], and produce various cytokines, like IL-6, IL-8, IL-15 and TNF [ 230 , 231 , 232 ]. As such, Caco-2 cells are widely used in IBD research [ 233 , 234 ].…”

Section: Screening Isolated Compounds For Anti-inflammatory Propertiesmentioning

confidence: 99%

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Approaches, Strategies and Procedures for Identifying Anti-Inflammatory Drug Lead Molecules from Natural Products

Jamtsho,

Yeshi,

Perry

et al. 2024

Pharmaceuticals

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Natural products (NPs) have played a vital role in human survival for millennia, particularly for their medicinal properties. Many traditional medicine practices continue to utilise crude plants and animal products for treating various diseases, including inflammation. In contrast, contemporary medicine focuses more on isolating drug-lead compounds from NPs to develop new and better treatment drugs for treating inflammatory disorders such as inflammatory bowel diseases. There is an ongoing search for new drug leads as there is still no cure for many inflammatory conditions. Various approaches and technologies are used in drug discoveries from NPs. This review comprehensively focuses on anti-inflammatory small molecules and describes the key strategies in identifying, extracting, fractionating and isolating small-molecule drug leads. This review also discusses the (i) most used approaches and recently available techniques, including artificial intelligence (AI), (ii) machine learning, and computational approaches in drug discovery; (iii) provides various animal models and cell lines used in in-vitro and in-vivo assessment of the anti-inflammatory potential of NPs.

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Section: Screening Isolated Compounds For Anti-inflammatory Propertiesmentioning

confidence: 99%

“…Ability produces various cytokines, including IL-6, IL-8, IL-15, TNF, and thymic stromal lymphopoietin (TSLP) [ 230 ].…”

Section: Figurementioning

confidence: 99%

Approaches, Strategies and Procedures for Identifying Anti-Inflammatory Drug Lead Molecules from Natural Products

Jamtsho,

Yeshi,

Perry

et al. 2024

Pharmaceuticals

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“…Differentiated Caco-2 cells produce the same characteristics and specific digestive enzymes (e.g., peptidase and lactase) as small-bowel enterocytes, such as brushborder microvilli [49]. They can also produce various cytokines (IL-6, -8, and -15, TNF-α, and thymic stromal lymphopoietin) [50][51][52]. In addition, absorption-related studies are easy to perform [53][54][55].…”

Section: In Vitro and Ex Vivo Ibd Modelsmentioning

confidence: 99%

Therapeutic Potential of Bioactive Components from Scutellaria baicalensis Georgi in Inflammatory Bowel Disease and Colorectal Cancer: A Review

Jang

Kim

2023

IJMS

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Scutellaria baicalensis Georgi (SBG), an herbal medicine with various biological activities, including anti-inflammatory, anticancer, antiviral, antibacterial, and antioxidant activities, is effective in treatment of colitis, hepatitis, pneumonia, respiratory infections, and allergic diseases. This herbal medicine consists of major active substances, such as baicalin, baicalein, wogonoside, and wogonin. Inflammatory bowel disease (IBD) comprises a group of inflammatory conditions of the colon and small intestine, with Crohn’s disease and ulcerative colitis being the main types. IBD can lead to serious complications, such as increased risk of colorectal cancer (CRC), one of the most common cancers worldwide. Currently, there is no cure for IBD, and its incidence has been increasing over the past few decades. This review comprehensively summarizes the efficacy of SBG in IBD and CRC and may serve as a reference for future research and development of drugs for IBD and cancer treatment.

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“…Questi risultati sono stati confermati determinando il flusso di isocianato di fluoresceina attraverso lo strato cellulare dopo il test della TEER (vedere Fig. 1) [3][4][5].…”

Section: Mgunclassified

“…Questa osservazione è stata supportata dalla rilevazione delle citochine pro-infiammatorie nei compartimenti su entrambi i lati dello strato cellulare nel modello di TEER [3].…”

Section: Mengen Pro Tagesdosisunclassified

La combinazione di probiotici, estratti vegetali e micronutrienti mostra effetti positivi sull'integrità della barriera intestinale

Barth,

Bulferi,

Lanza

et al. 2023

Evid Self Med

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I disturbi funzionali del tratto gastrointestinale causano sintomi che possono avere un impatto significativo sulla qualità di vita delle persone affette. Mantenere una barriera intestinale integra è essenziale per la prevenzione e il trattamento di questi disturbi. I primi studi mostrano risultati promettenti sull'effetto sinergico di una combinazione di batteri probiotici ed estratti vegetali.

show abstract

Anti-inflammatory activity of a fixed combination of probiotics and herbal extract in an in-vitro model of intestinal inflammation by stimulating Caco-2 cells with LPS-conditioned THP-1 cells medium

Cited by 6 publications

References 42 publications

Approaches, Strategies and Procedures for Identifying Anti-Inflammatory Drug Lead Molecules from Natural Products

Approaches, Strategies and Procedures for Identifying Anti-Inflammatory Drug Lead Molecules from Natural Products

Therapeutic Potential of Bioactive Components from Scutellaria baicalensis Georgi in Inflammatory Bowel Disease and Colorectal Cancer: A Review

La combinazione di probiotici, estratti vegetali e micronutrienti mostra effetti positivi sull'integrità della barriera intestinale

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