Anti-inflammatory activity of Bacopa monniera in rodents

Channa, Shabana; Dar, Ahsana; Anjum, Shazia; Yaqoob, Muhammad; Atta-ur-Rahman, _

doi:10.1016/j.jep.2005.10.009

Cited by 96 publications

(47 citation statements)

References 13 publications

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“…Bacoside A is a mixture of bacosaponin isomers. These active components facilitate learning and memory and also possess antiamnesic, antistress (Chowdhuri et al 2002), anxiolytic (Ernst 2006), antidementic (Dhawan and Singh 1996), antiulcerogenic, antiarthritis, anti-inflammatory (Channa et al 2006), antifatigue (Anand et al 2012) and neuroprotective properties (Dhanasekharan et al 2007;Pandareesh and Anand 2013). We previously demonstrated the antioxidant and plasmid DNA damage preventive properties of hexane, chloroform, ethyl acetate, acetone, methanol and aqueous extracts from B. monniera (Anand et al 2011).…”

Section: Introductionmentioning

confidence: 98%

Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells

2014

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Hydrogen peroxide (H 2 O 2 ), a major reactive oxygen species (ROS) produced during oxidative stress, is toxic to the cells. Hence, H 2 O 2 has been extensively used to study the effects of antioxidant and cytoprotective role of phytochemicals. In the present investigation H 2 O 2 was used to induce oxidative stress via ROS production within PC12 and L132 cells. Cytoprotective propensity of Bacopa monniera extract (BME) was confirmed by cell viability assays, ROS estimation, lipid peroxidation, mitochondria membrane potential assay, comet assay followed by gene expression studies of antioxidant enzymes in PC12 and L132 cells treated with H 2 O 2 for 24 h with or without BME pre-treatment. Our results elucidate that BME possesses radical scavenging activity by scavenging 2,2-diphenyl-1-picrylhydrazyl, 2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide radical, and nitric oxide radicals. The IC 50 value of BME against these radicals was found to be 226.19, 15.17, 30.07, and 34.55 lg/ml, respectively). The IC 50 of BME against ROS, lipid peroxidation and protein carbonylation was found to be 1296.53, 753.22, and 589.04 lg/ml in brain and 1137.08, 1079.65, and 11101.25 lg/ml in lung tissues, respectively. Further cytoprotective potency of the BME ameliorated the mitochondrial and plasma membrane damage induced by H 2 O 2 as evidenced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase leakage assays in both PC12 and L132 cells. H 2 O 2 induced cellular, nuclear and mitochondrial membrane damage was restored by BME pre-treatment. H 2 O 2 induced depleted antioxidant status was also replenished by BME pre-treatment. This was confirmed by spectrophotometric analysis, semi-quantitative RT-PCR and western blot studies. These results justify the traditional usage of BME based on its promising antioxidant and cytoprotective property.

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Section: Introductionmentioning

confidence: 98%

Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells

2014

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“…The time line of edema development in carrageenan induced paw edema model in rats is commonly represented by a biphasic curve. The first phase occurs within an hour of injection and is partly due to the trauma of injection and partly due to the release of 5-HT, histamine and kinins [24][25][26][27][28]. The anti-inflammatory effect of M. zapota extract was apparent in each concentration of extracts as early as the first hour of carrageenan injection and highest inhibition was during the fifth hour.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Manilkara Zapota Leaf Extract

Konuku

Karri

Gopalakrishnan

et al. 2017

Int J Curr Pharm Sci

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Objective: Manilkara zapota is a medicinal plant which is native to Mexico and Central America, and widely distributed in India. Various parts of this plant are traditionally used for treatment of several diseases, including inflammation-associated ailments. The main aim of the present study is to evaluate the anti-inflammatory potential of ethyl acetate and methanolic extracts of M. zapota leaf. Methods:In vitro secretary phospholipase A2 (PLA2) and 5-Lipoxygenase (5-LOX) assays and In vivo studies using carrageenan induced rat paw edema model were performed to assess the anti-inflammatory activity of M. zapota leaf extracts. Results:In vitro studies suggest that M. zapota leaf extracts exhibited significant SPLA2 and 5-LOX inhibitory activities. In in vivo studies M. zapota leaf extracts showed dose dependent inhibition of carrageenan induced paw edema in rats. The anti-inflammatory activity of ethyl acetate leaf extract was superior to methanolic extract. Conclusion:This study concluded that ethyl acetate leaf extract of M. zapota exhibited significant anti-inflammatory activity and warranted further investigation to isolate and identify the components.

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“…An ethanolic extract of BM reduce lipid peroxidation and promotes antioxidant status. 24 Based on ethnopharmacological uses, we investigated the anti-inflammatory effects of BM ethanol extract in an in vivo model of UC induced by AA in CF rats. This model exhibits symptoms comparable to those of human ulcerative colitis, such as: weight loss, diarrhea, bloody faeces, mucosal ulceration and also serves as a model to investigate the role of free radicals and antioxidants in the pathophysiology of colitis.…”

Section: Discussionmentioning

confidence: 99%

The role of antioxidants and free radicals in the healing effects of Bacopa monniera on acetic acid-induced colitis in rats

Sharma

Chauhan

Singh

et al. 2017

Int J Basic Clin Pharmacol

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Background: The aim to study and elucidate the healing effects of ethanolic extract of dried whole plant of Bacopa monniera against experimental colitis in rats.Methods: Bacopa monniera whole plant extract was administered orally, once daily for 14 days, to rats after induction of colitis with acetic acid. We studied its effects on: faecal output, food and water intake, and body weight changes and also examined colonic mucosal damage, inflammation and status of antioxidants: superoxide dismutase, reduced glutathione; free radicals: nitric oxide, lipid peroxidation on 15th day of the experiment. Antibacterial activity of the extract was also studied using in vitro procedures. Statistical comparison was performed using either unpaired ‘t’ test or one -way analysis of variance (ANOVA) and for multiple comparisons versus control group was done by Dunnett’s test.Results: Bacopa monniera whole plant extract decreased colonic mucosal damage, inflammation, faecal output and increased body weight in acetic acid induced colitis. It also showed antibacterial activity and enhanced the antioxidant but decreased free radicals. Acute toxicity study indicated no mortality or other ANS or CNS related adverse effects even with ten time effective dose indicating its safety.Conclusions: Bacopa monniera whole plant extract is safe, effective and could be beneficial as a complementary agent in treatment of ulcerative colitis.

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Anti-inflammatory activity of Bacopa monniera in rodents

Cited by 96 publications

References 13 publications

Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells

Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells

Anti-Inflammatory Activity of Manilkara Zapota Leaf Extract

The role of antioxidants and free radicals in the healing effects of Bacopa monniera on acetic acid-induced colitis in rats

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