Anti-inflammatory Activity of Baicalein in LPS-Stimulated RAW264.7 Macrophages via Estrogen Receptor and NF-κB-Dependent Pathways

Fan, Guanwei; Zhang, Yuan; Jiang, Xiaorui; Zhu, Yan; Wang, Bingyao; Su, Linlin; Cao, Wenjie; Zhang, Han; Gao, Xiumei

doi:10.1007/s10753-013-9703-2

Cited by 105 publications

(64 citation statements)

References 29 publications

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“…Moreover, TNF-α is one of the most potent innate immune-activating stimuli. TNF-α exerts its toxic effects by potently activating HSC and inducing the expression of inflammatory cytokines such as IL-1β and IL-6 [21,22]. Our study also confirmed P-Smad3 an upregulated expression of IL-1β, IL-6, and TNF-α in LX-2 cells induced by TNF-α.…”

Section: Discussionsupporting

confidence: 82%

NLRC5 Mediates IL-6 and IL-1β Secretion in LX-2 Cells and Modulated by the NF-κB/Smad3 Pathway

Huang

et al. 2015

Inflammation

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Recent data have shown that nucleotide-binding domain leucine-rich repeat proteins (NLRs), a class of innate immune receptors that respond to pathogen attack or cellular stress, have gained increasing attention. NLRC5 (NLR family, CARD domain containing 5) is the largest member of the NLR family, which has recently been identified as a critical regulator of immune responses. Until recently, the function of NLRC5 has been a matter of debate. In this study, we explore the role of NLRC5 in cytokine secretion and the role of the nuclear factor-κB (NF-κB) signaling pathway in tumor necrosis factor-alpha (TNF-α)-induced NLRC5 expression in LX-2 cells. We demonstrated that overexpression of NLRC5 results in an upregulation of IL-6 and IL-1β secretion. On the other hand, knockdown of NLRC5 by transfecting siRNA decreased IL-6 and IL-1β secretion in LX-2 cells. Meanwhile, the results showed that pyrrolidine dithiocarbamate (PDTC) (a specific inhibitor of the NF-κB signaling pathway) inhibited NLRC5 expression and NLRC5 silencing could increase the expression levels of p65 in cell nucleus accompanied with upregulated phosphorylation of Smad3 protein levels in response to TNF-α. These results indicated that NLRC5 plays a significant role in TNF-α-enhanced cytokine (IL-6 and IL-1β) secretion of LX-2 cells and the NF-κB/Smad3 signal pathway is involved in its induction of expression.

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Section: Discussionsupporting

confidence: 82%

NLRC5 Mediates IL-6 and IL-1β Secretion in LX-2 Cells and Modulated by the NF-κB/Smad3 Pathway

Huang

et al. 2015

Inflammation

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“…Baicalein is a flavonoid isolated from Scutellaria baicalensis Georgi (Huangqin)which possesses anti-inflammatory, anti-oxidative and anti-carcinogenic activities [7][8][9][10]. In additon, baicalein has been shown to attenuate MMP-1 expression in human keratinocytes [11].…”

Section: Introductionmentioning

confidence: 99%

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Chen

Xiong

et al. 2015

Cell Physiol Biochem

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Background: Baicalein is a flavonoid isolated from Scutellaria baicalensis Georgi. Here, we investigated the anti-osteoarthritic effect of baicalein in vitro and in vivo. Methods: Interleukin-1 beta (IL-1β)-induced chondrocytes were treated with different concentrations of baicalein, real-time PCR and ELISA were performed to detect the matrix metalloproteinases (MMPs) expression. Western blot was used to evaluate the mitogen-activated protein kinase (MAPK) expression. In experimental osteoarthritis (OA), rabbits were treated with baicalein, gross morphological and histological assessment was performed to evaluate the cartilage damage. Results: Baicalein significantly reduced the expression of MMPs in vitro and in vivo. Moreover, baicalein significantly reduced the phosphorylation of p38 and extracellular signal regulated kinase (ERK), but not of c-Jun N-terminal kinase (JNK). In addition, intra-articular injection of baicalein ameliorated the cartilage damage in a rabbit model of OA induced by anterior cruciate ligament transection (ACLT). Conclusions: The results indicate that baicalein may be considered as a potential agent for OA treatment.

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“…Baicalein (5,6,7-trihydroxyflavone) is an active ingredient extracted from the traditional Chinese herb Scutellaria baicalensis Georgi, which has been reported to possess anti-inflammatory, anti-oxidant, anti-cancer and anti-type 2 diabetes properties and is widely used to treat various diseases (Lin, Shen, Lin, Yang, & Chen, 2007;Li-Weber, 2009;Fan et al, 2013;Fu et al, 2014). Baicalein has been reported to stimulate osteoblastic MC3T3-E1 cell differentiation, inhibit osteoclast A c c e p t e d M a n u s c r i p t differentiation and induce mature osteoclast apoptosis to promote bone metabolism (Kim, Lee, Kim, Min, & Kim, 2008;.…”

Section: Introductionmentioning

confidence: 99%

Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/β-catenin signaling pathway

Chen

Shi

et al. 2017

Archives of Oral Biology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  The optimal dosage of baicalein was 10 μM. DKK-1 significantly reversed the effects of baicalein on hPDLCs. The effects are exerted by activating the Wnt/β-catenin signaling pathway. Page 3 of 29A c c e p t e d M a n u s c r i p t ABSTRACT Objective: Periodontium regeneration is one of the most important processes for periodontitis therapy. Human periodontal ligament cells (hPDLCs) play a vital role in the repair and regeneration of periodontal tissues. Our study aimed to investigated the mechanisms underlying the promotion of hPLDCsosteogenic differentiation by baicalein. Design: hPDLCs were obtained from periodontal ligament (PDL) tissues by primary culture. The MTT assay was used to determine the growth curves of hPDLCs treated with different concentrations of baicalein (1.25, 2.5, 5, or 10 μM). Alkaline phosphatase (ALP) staining and Alizarin red S staining were performed to assess osteogenic differentiation of hPDLCs administered baicalein. Osteogenic differentiation-related gene and protein expression levels and Wnt/β-catenin pathway signal changes were assessed by qRT-PCR and Western blotting analysis. Results:The results showed that baicalein decreased the growth of hPDLCs slightly and increased ALP activity and calcium deposition in a dose-dependent manner. The expression of runt-related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), Osterix (OSX) and osteocalcin (OCN) were elevated after baicalein administration. Moreover, baicalein strongly activated the Wnt/β-catenin pathway and up-regulated the expression of β-catenin, lymphoid enhancer factor 1 (LEF1) and Cyclin D1. Dickkopf-related protein 1 (DKK-1) significantly reversed the effects of baicalein on hPDLCs. Conclusions: Our findings indicated that baicalein enhanced the osteogenic differentiation of hPDLCs via the activation of the Wnt/β-catenin signaling pathway, which may represent a potential candidate for periodontitis therapy.

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Anti-inflammatory Activity of Baicalein in LPS-Stimulated RAW264.7 Macrophages via Estrogen Receptor and NF-κB-Dependent Pathways

Cited by 105 publications

References 29 publications

NLRC5 Mediates IL-6 and IL-1β Secretion in LX-2 Cells and Modulated by the NF-κB/Smad3 Pathway

NLRC5 Mediates IL-6 and IL-1β Secretion in LX-2 Cells and Modulated by the NF-κB/Smad3 Pathway

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/β-catenin signaling pathway

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