Anti‐inflammatory activity of bee venom peptide 401 (mast cell degranulating peptide) and compound 48/80 results from mast cell degranulation in vivo

Abstract

5 The anti-inflammatory activity of peptide 401 and of compound 48/80 in the carrageenin-induced swelling of the rat hind paw arises from mast cell degranulation in vivo.

A role for mast cells in adenosine A3 receptor‐mediated hypotension in the rat

“…It is, however, entirely consistent with in vitro findings where mediator release inhibition is often incomplete and may show a bell-shaped doseresponse relationship (Johnson et al, 1978;Tanizaki et al, 1992). defining the role of the mast cell in a variety of experimental circumstances (see, for example, Di Rosa et al, 1971;Banks et al, 1990). The pretreatment paradigm used in the present experiments (1 mg kg-', i.p.…”

“…It is, however, entirely consistent with in vitro findings where mediator release inhibition is often incomplete and may show a bell-shaped doseresponse relationship (Johnson et al, 1978;Tanizaki et al, 1992). defining the role of the mast cell in a variety of experimental circumstances (see, for example, Di Rosa et al, 1971;Banks et al, 1990 the A3 receptor-mediated component of the response to AP-NEA, neither increased histamine levels per se nor modified the response to APNEA, mediator release can be unequivocally attributed to the effects of APNEA. It bears emphasis that the implication from these in vivo studies is that A3 receptor activation is per se sufficient to activate powerfully mast cell mediator release whereas from in vitro studies the predominant effect is to augment mediator release induced by stimuli such as allergen and compound 48/ 80 (Marquardt et al, 1978;Ali et al, 1990;Ramkumar et al, 1993).…”

Mechanism of airway hyperresponsiveness to adenosine induced by allergen challenge in actively sensitized Brown Norway rats

“…Third, the response to adenosine augmented following allergen challenge was suppressed in animals treated with compound 48/80, a mast cell degranulating agent ( Paton, 1951 ). Repeated treatment with compound 48/80 to achieve depletion of mast cell‐derived mediators has been a successful strategy in defining a role of the mast cell in a variety of experimental paradigms ( Banks et al ., 1990 ; Hannon et al ., 1995 ; Meade et al ., 1996 ; Reeves et al ., 1997 ). We used a pretreatment schedule which resulted in extensive depletion of skin mast cells as quantified by inhibition of plasma protein extravasation ( Reeves et al ., 1997 ).…”

The nociceptive and anti-nociceptive effects of bee venom injection and therapy: A double-edged sword

“…1, Table 1), a bee venom polypeptide with 22 amino acids and constituting 2–3% of dry bee venom, was originally named due to its biological action of causing release of histamine from mast cells (Jasani et al, 1979; Banks et al, 1990). However, studies of its biological and pharmacological actions in the CNS have been emerging gradually.…”

“…3). One alternative mechanism is via mast cell degranulation caused by MCD peptide (or melittin) (Jasani et al, 1979; Banks et al, 1990) and tissue damage caused by hyaluronidase (Habermann, 1957; Barker et al, 1963; Kemeny et al, 1984). Following the above actions, many pain-producing substances such as histamine, bradykinin, 5-HT, ATP, K + and protons can be released and act on their respective receptors and channels, leading to persistent activation of nociceptor cells (Fig.…”