Anti-Inflammatory Activity of Benzene Extract of Abies Webbiana Lindl. (Fam-Pinaceae) Leaves

Sahoo, Nityananda

doi:10.20959/wjpps20179-9951

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…From phytochemical investigation, biologically active principles such as flavonoids, tannins, phenolic compounds, and phytosterols in plants are revealed. It is suggested that one of the above constituents or a combination is responsible for producing the analgesic and [53] anti-inflammatory effects. Few studies show that the anti-inflammatory property of A. webbiana might be due to flavonoids or even steroids that are present in them [54,55].…”

Section: Discussionmentioning

confidence: 99%

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In vitro Anti Inflammatory Activity of Abies webbiana Using Albumin Denaturation Assay

Anjum

Varghese

Rajeshkumar

2021

JPRI

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Background: In ayurveda, many natural plant components are used to treat inflammatory conditions for centuries with less side effects. Different parts of Abies webbiana plant are used in ayurveda for many diseases. However, very few studies have been conducted to evaluate pharmacological effects of Abies webbiana. Aim: The objective of this in vitro study was to analyse and compare the anti-inflammatory properties of A. webbiana at various concentrations using albumin denaturation assay. Methodology: A ready made powder of A. webbiana was mixed with 100 ml of distilled water and boiled for 10 mins the extract was obtained by filtration. The anti-inflammatory activity was done using albumin denaturation assay and Diclofenac sodium was used as a positive control. Serial dilution from 10 µL, 20 µL, 30 µL, 40 µL and 50 µL was performed for A. webbiana and the reference drug reaction mixture was prepared for albumin denaturation assay. All the data was analysed statistically using SPSS version 23 (IBM). Unpaired t test was used for comparison with the positive control and one-way Anova followed by Tukey’s post hoc test were used for the comparison between different concentrations. Results: The current study revealed that A. webbiana extract had potent anti-inflammatory activity at all the tested concentrations but was significantly lesser when compared to the standard (p<0.05). It was also evident that the absorbance rate of A. webbiana significantly increased with the increase in concentration of the extract (p<0.05). However, the absorbance rate at 50 µL of both standard and A. webbiana were almost similar. Conclusion: It can be concluded from the present findings that the leaves of A. webbiana possess concentration-dependent anti-inflammatory property but is comparatively lesser than Diclofenac sodium.

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Section: Discussionmentioning

confidence: 99%

“…Anti inflammatory activity of A. webbiana has not been reported in many studies in literature. It was only studied on rats [53]. There are several other properties of A. webbiana such as antimicrobial and antitumor agents as well used in ayurveda [59].…”

Section: Discussionmentioning

confidence: 99%

In vitro Anti Inflammatory Activity of Abies webbiana Using Albumin Denaturation Assay

Anjum

Varghese

Rajeshkumar

2021

JPRI

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“…The antiinflammatory effect was exhibited by pinitol which was isolated from the leaf. A new aziridine alkaloid was isolated from the leaves of A. webbiana [55]. Moreover, it has been reported that large number of different chemical compounds such as phenolic compounds and derivative compounds, the esters of weak acid, fatty acid, terpenes, and others are presented in ethanolic extracts of this plants and thus these chemical components can affect multiple target sites against the bacterial cells [56].…”

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confidence: 99%

Abies webbiana Ethanolic Extract Based Mouthwash and Its Antimicrobial and Cytotoxic Effect

Timothy

Nandhini

Varghese

et al. 2021

JPRI

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Aim: The aim of this study was to evaluate the antimicrobial and cytotoxic effect of Abies webbiana ethanolic extract based preparation of mouthwash and to compare these effects between different concentrations. Background: Abies webbiana leaves have been used in traditional siddha and ayurveda systems of medicine for common ailments such as cough, loss of appetite, indigestion, mental disorders, rheumatism, bronchitis, pulmonary infection, asthma, antiseptic and decongestant. Abies webbiana commonly known as talispatra belongs to family Pinaceae. The plant is distributed throughout India, mostly in the Himalayan region from Kashmir to Assam states. Materials and Methods: The preparation of Abies webbiana extract was done and antimicrobial activity of Abies webbiana were tested with Streptococcus mutans, Candida albicans, Enterococcus faecalis, Staphylococcus aureus through agar well diffusion method. Each bacterial culture was spread on the Luria-Bertani agar plates. Dried extract of Abies webbiana, dissolved in autoclave distilled water to make 25mg/mL, 50mg/mL, 100mg/mL. Incubated for 24 hours at 37o C and diameter of zone of inhibition were recorded in millimeter and compared with standard amoxicillin. Cytotoxic activity of Abies webbiana was assessed using brine shrimp lethality assay. A total of 10 nauplii were added into the three replicates of each concentration of the prepared extract. After the period of 24 hours the remaining brine shrimp were counted. Results: The results of the study revealed that the the Abies webbiana formulation based mouthwash is having significantly higher antimicrobial activity than the antibiotic against S. aureus at all the tested concentrations whereas against E. faecalis the antimicrobial effect was significantly lower than the antibiotic. Against S. mutans, higher concentrations of the mouthwash had the antimicrobial effect comparable to that of the antibiotic. The antimicrobial effect against C. albicans was very less for all the tested concentrations of the mouthwash and the antibiotic. This mouthwash also possessed cytotoxic effects on nauplii which were within normal limits and it was found that the nauplii alive was directly proportional to the concentrations except in the concentration of 40μl. Conclusion: Within the limitations of the current study, ethanolic extract of Abies webbiana based preparation of mouthwash shows the antimicrobial activity almost comparable to amoxicillin. It was also revealed that it possesses less cytotoxic effect against brine shrimp which are within normal limits.

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Anti-Inflammatory Activity of Benzene Extract of Abies Webbiana Lindl. (Fam-Pinaceae) Leaves

Cited by 2 publications

References 4 publications

In vitro Anti Inflammatory Activity of Abies webbiana Using Albumin Denaturation Assay

In vitro Anti Inflammatory Activity of Abies webbiana Using Albumin Denaturation Assay

Abies webbiana Ethanolic Extract Based Mouthwash and Its Antimicrobial and Cytotoxic Effect

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