Anti-inflammatory activity of chemical constituents from Echinops gracilis (Asteraceae)

Abstract

The present work concerns the anti-inflammatory activity of chemical constituents isolated from the roots of Echinops gracilis. The anti-inflammatory activity of the ethyl acetate extract as well as compounds 1, 2, and 3 were evaluated using inhibition of protein denaturation and antiproteinase methods. Structural elucidation was achieved through analysis of NMR spectra (1H and 13C, 1H1H COSY, HSQC, HMBC) and literature survey. Seven compounds were identified as erythrinasinate (1), vogelate (2), ferulic acid (3), p-coumaric acid (4), ursolic acid (5), oleanolic acid (6), and quercetin (7). All the isolated compounds were reported for the first time from this plant. Ethyl acetate extract showed potent inhibitory activity against protein denaturation (IC50 = 125.54 µg/mL). Erythrinasinate (1) and vogelate (2) showed a significant anti-inflammatory activity with an IC50 value of 469.43 and 413.71 µg/mL, respectively. The results obtained from the ethyl acetate extract can justify the use of E. gracilis roots in traditional medicine for the treatment of rheumatism.

Antibacterial and Antioxidant Activities of the Extract and Some Flavonoids From Aerial Parts of Echinops Gracilis O. Hoffm. (Asteraceae)

“…Moreover, the anti-inflammatory activity of E. gracilis extracts and some isolated compounds has been established. 10 We herein report the isolation and structural elucidation of one new…”

“…Moreover, the anti-inflammatory activity of E. gracilis extracts and some isolated compounds has been established. 10 We herein report the isolation and structural elucidation of one new flavonoid derivative; apigenin-7-O-(4"-feruloyl)-β-Dglucoside (1) and 2 known compounds apigenin-7-O-(4"-trans-phydroxycinnamoyl)-β-D-glucoside (2) and apigenin-7-Oglucoside (3) from the aerial parts of E. gracilis. Considering the traditional uses and the in vitro antimicrobial activities 2 of some Echinops species such as E. amplexicaulis, E. giganteus, and E. keberiko, and to contribute to the global fight against microbial diseases, antibacterial and antioxidant tests were performed on the ethyl acetate extract, as well as some isolated compounds of E. gracilis.…”

“…Moreover, the anti-inflammatory activity of E. gracilis extracts and some isolated compounds has been established. 10…”