Anti-inflammatory activity of ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. and their phenolic profile

Deghima, Amirouche; Righi, Nadjat; Rosales-Conrado, Noelia; León-González, María Eugenia; Baali, Faiza; Gómez-Mejía, Esther; Madrid, Yolanda; Bedjou, Fatiha

doi:10.1016/j.jep.2020.113347

Cited by 18 publications

(11 citation statements)

References 37 publications

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“…This result further justifies the previously studied correlation between phenolic phytoconstituents and free radical neutralizing properties; antioxidant potential of a plant in general (Casquete et al, 2015). As flavonoids and diterpenes isolated from plant source be related to their antioxidant potential (Alam et al, 2021;Deghima et al, 2021) along with existing reports on the isolation of said compounds from other plants of the genus (Chen et al, 2009), it can be fairly speculated that flavonoids or diterpenes present in B. Clarkei leaves might be responsible for its antioxidant activity.…”

Section: Total Phenolic Contentsupporting

confidence: 83%

In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

et al. 2021

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As there is a resurgence of interest in plants as promising sources of new active pharmaceutical constituents, the present study has been designed to establish the preliminary biological activities of Blumea clarkei Hook.f. leaves. Here, the crude methanol extract of the leaves along with its organic and aqueous soluble fractions were subjected to different in vitro and in vivo assays. The ethyl acetate soluble fraction exhibited the highest total phenolic content (71.59 mg of GAE/gm of extract) and DPPH free radical scavenging (IC50 value 19.25 μg/ml) whereas another polar fraction; the aqueous soluble extractive showed the maximum cytotoxicity (LC50 value of 18.60 μg/ml) in the brine shrimp lethality assay. In membrane stabilizing activity evaluation, the inhibition of hypotonic solution and heat induced hemolysis were revealed maximum by the DCM (30.64%) and aqueous soluble fraction (39.15%), respectively while the crude methanol extract exhibited the highest thrombolytic potential (30.47% clot lysis). The central analgesic activity of the crude extract significantly increased the latent response time in tail-immersion method (p<0.001) at doses 200 and 400 mg/kg of body weight after 90 minutes of administration when compared to the control group. In acetic acid-induced writhing method, the plant extract showed prominent peripheral analgesia (p<0.001) with 47.54% and 65.57% inhibition at 200- and 400-mg/kg of body weight, respectively. In addition, a dose dependent hypoglycemic and antidiarrheal activities were also observed by the crude extract. Bangladesh Pharmaceutical Journal 24(2): 149-158, 2021

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Section: Total Phenolic Contentsupporting

confidence: 83%

In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

et al. 2021

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“…However, the best anti-inflammatory activities were recorded for the leaf extracts of the two analyzed species ( Table 3 ). Previous studies have shown that polyphenols, in particular flavonoids, can mediate interactions with the phospholipidic cytoplasmic membrane, thereby reducing fluidity and enhancing rigidity [ 59 , 62 ]. These interactions protect the membrane from harmful substances, thus ensuring the physiological function and integrity of the membrane [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported a weak correlation between polyphenol content and membrane stabilizing activity (R 2 = 0.435) [ 59 ]. The membrane stabilization potential of the C. sieberiana and P. thonningii extracts could be due to the high content of phenolic compounds in particular flavonoids, compounds that play an important role in the prevention of the membrane lipid peroxidation against hypotonic and thermal lysis [ 59 , 62 , 65 ]. These antioxidants, such as flavonoids, polyphenols, and vitamin C, can interact with pro-inflammatory cytokines or can block active sites of COX, LOX, arachidonic acid secretion, and production of prostaglandins and leukotrienes, resulting in a low level of reactive oxygen species and subsequent oxidative stress reduction ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Exploration of the Antioxidant and Anti-inflammatory Potential of Cassia sieberiana DC and Piliostigma thonningii (Schumach.) Milne-Redh, Traditionally Used in the Treatment of Hepatitis in the Hauts-Bassins Region of Burkina Faso

et al. 2023

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Inflammation is the supreme biological response to illness. In the Hauts-Bassins region, in traditional medicine, all parts of Cassia sieberiana and Piliostigma thonningii are used to treat hepatitis and inflammation. The aim of this study was to evaluate the in vitro antioxidant and anti-inflammatory activities of their aqueous extracts. High performance liquid chromatography with photodiode array (HPLC-DAD) and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-MS/MS) analyses highlighted the presence of polyphenols and flavonoids. Antioxidant and anti-inflammatory activities were measured by various methods such as DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), TAC (total antioxidant capacity), anti-protease, anti-lipoxygenase, and membrane stabilization. The best antioxidant activity was observed in the bark (DPPH: IC50 = 13.45 ± 0.10µg/mL) and roots (TAC = 29.68 ± 1.48 mgAAE/g DW) of Piliostigma thonningii and in the roots (ABTS: IC50 = 1.83 ± 0.34 µg/mL) of Cassia sieberiana. The best anti-inflammatory activity was observed in the bark (anti-lipoxygenase: IC50 = 13.04 ± 1.99 µg/mL) and leaves (anti-proteases: IC50 = 75.74 ± 1.07 µg/mL, membrane stabilization: IC50 = 48.32 ± 6.39 µg/mL) of Cassia sieberiana. Total polyphenols (ABTS: r = −0.679, TAC: r = 0.960) and condensed tannins (ABTS: r = −0.702, TAC: r = 0.701) were strongly correlated with antioxidant activity. Total flavonoids (anti-proteases: r = −0.729), condensed tannins (anti-proteases: r = 0.698), and vitamin C (anti-proteases: r = −0.953) were strongly correlated with anti-inflammatory activity. Total polyphenols, flavonoids, condensed tannins, and vitamin C could contribute to the antioxidant and anti-inflammatory activities of the two studied plants. These results could validate the traditional use of these plants to treat various inflammatory diseases.

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“…The selection of appropriate solvents for extraction is the key factor in natural product research. In this study, the chloroform and n -butanol solvents were used due to previous evidence of the role of the solvents in extract preparation with a rich quantity of polyphenols [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition and Biological Evaluation of Typha domingensis Pers. to Ameliorate Health Pathologies: In Vitro and In Silico Approaches

Dilshad

Khan

Saeed

et al. 2022

BioMed Research International

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Human diseases are becoming more prevalent, necessitating the development of modalities to overcome the challenges of treating various disorders. In the current research, we analyzed the biomedicinal role of Typha domingensis which is an important medicinal plant. The species is traditionally used in the treatment of neurological disorders and skin malignancies. The chloroform (CFTD) and n-butanol fractions of T. domingensis (BFTD) were subjected to chemical profiling through the determination of total polyphenolic contents and GC-MS analysis. The oral toxicity test was applied to investigate the toxicity of the extracts. Antioxidant capacity was analyzed by four in vitro methods: DPPH, ABTS, FRAP, and CUPRAC. The pharmacological potential was evaluated through clinically significant enzyme inhibition assays, thrombolytic, and antimicrobial activities. In silico molecular docking approach was applied to confirm the role of T. domingensis against the enzymes. The polyphenolic quantification revealed that the BFTD was comparatively rich in total phenolic and flavonoid contents (97.14 milligrams gallic acid equivalent (mg GAE/g) and 362.5 milligrams quercetin equivalent per gram of dry extract (mg QE/g DE), respectively), as compared to the CFTD. The GC-MS analysis of the CFTD and BFTD resulted in the tentative identification of 67 and 29 compounds, respectively, with the major components of fatty acids and essential oil. The oral toxicity test revealed the safety and biocompatibility of CFTD and BFTD. Both the fractions showed promising antioxidant activity. Tyrosinase was found as the major enzyme inhibited by BFTD (78.67%) and CFTD (68.09%), whereas the standard kojic acid showed 85.58% inhibition. The inhibition results of acetylcholinesterase and butyrylcholinesterase by BFTD (71.65 and 60.79%, respectively) are higher than CFTD. Both the fractions were found active against various strains of bacteria. Furthermore, the molecular docking studies of the compounds showed a good docking score against all the docked enzymes among which deoxycaesaldekarin C was found with the highest binding affinities in comparison to the standard. The current study suggests that T. domingensis is nontoxic and can be a potential source of phytoconstituents with promising pharmacological potential.

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Anti-inflammatory activity of ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. and their phenolic profile

Cited by 18 publications

References 37 publications

In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

Exploration of the Antioxidant and Anti-inflammatory Potential of Cassia sieberiana DC and Piliostigma thonningii (Schumach.) Milne-Redh, Traditionally Used in the Treatment of Hepatitis in the Hauts-Bassins Region of Burkina Faso

Chemical Composition and Biological Evaluation of Typha domingensis Pers. to Ameliorate Health Pathologies: In Vitro and In Silico Approaches

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