Anti-inflammatory activity of flavonoids from Eupatorium arnottianum

Clavin, M.; Gorzalczany, Susana; Macho, Antonio; Muñóz, Eduardo; Ferraro, Graciela; Acevedo, Cristina; Martino, Virginia

doi:10.1016/j.jep.2007.04.007

Cited by 119 publications

(86 citation statements)

References 21 publications

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“…7,8) Previous studies reported the presence of the following compounds in EB: scutellarin, erigeroside, quercetin, apeginin, pyromeconic acid, etc. 9 12) Considering the antiin‰ammatory activities of these ‰avonoids, 35,36) it was assumed that similar antiin‰ammatory eŠects of EM and EB in cotton pellet-induced granuloma and carrageenan-induced rat paw edema could be attributed to those similar ‰avonoids. On the other hand, the diŠerent inhibition rate of EM and EB in the xylene-induced mouse ear edema model may be caused by the diŠerent levels of ‰avonoids they contain.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Antiinflammatory Activities of Three Medicinal Plants Known as “Meiduoluomi” in Tibetan Folk Medicine

Zhang

Luo

Li³

et al. 2008

YAKUGAKU ZASSHI

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Erigeron breviscapus (Vant.) Hand-mazz (EB), Erigeron multiradiatus (Lindl.) Benth (EM), and Aster brachytrichus Franch (AB), confused under the vernacular name``meiduoluomi'' by native people and traditional healers, have been used for the treatment of meningitis, polyneuritis, hepatitis, adenolymphitis, and enteronitis in traditional Tibetan medicine. In this study, the antiin‰ammatory activity of methanol extracts of all three plants was investigated in the xylene-induced ear edema model, carrageenan-induced paw edema model, and cotton pellet-induced granuloma model. It was found that the methanolic extracts of both EB and EM had strong inhibitory eŠects on the acute phase of in‰ammation in carrageenan-induced paw edema in rats. On the other hand, the methanolic extract of EM showed stronger eŠects than those of EB in xylene-induced ear edema. In the chronic test, the methanolic extracts of EB and EM resulted in a signiˆcant reduction in granuloma weight in rats. In addition, myeloperoxidase (MPO) activity was strongly reduced in the EB-treated and EM-treated groups, which indicated that EB and EM can inhibit certain in‰ammatory modulator factors that cause neutrophil aggregation in in‰amed tissue, e.g., nuclear factor-kB. However, the methanolic extracts of AB had no antiin‰ammatory eŠects in the tested models and MPO assay. The similar eŠects of EM and EB in tested models provided some scientiˆc basis for the traditional usage of meiduoluomi in in‰ammatory disease. However, the results also suggest that further study is needed to investigate the antiin‰ammatory proˆle of AB and provide a scientiˆc basis for the use of AB in in‰ammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Antiinflammatory Activities of Three Medicinal Plants Known as “Meiduoluomi” in Tibetan Folk Medicine

Zhang

Luo

Li³

et al. 2008

YAKUGAKU ZASSHI

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“…There are lots of reports which claim that natural flavonoids and flavanones exhibit a widespectrum of biological activities including antimicrobial, anti-inflammatory and anti-cancer activities (Ley et al, 2005;Ngandeu et al, 2008;Clavin et al, 2007;Havsteen, 1983). The anti-cancer and apoptotic activi-…”

Section: Introductionmentioning

confidence: 99%

Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Wang

et al. 2016

Bangladesh J Pharmacol

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The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein.Video Clip<a href="https://youtube.com/v/MJwBotwrgWM">Flow cytometry assay</a>: 35 sec

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“…Polyphenols are plant secondary metabolites widespread in the plant kingdom. They have many benefi cial properties proved in in vitro and in vivo studies, namely: antibacterial [Daglia et al, 2007], antifungal [Báidez et al, 2006], neuroprotective [Kumar et al, 2007] and also anticancer, in particular: antioxidant [Robaszkiewicz et al, 2007], antimetastatic [Ogasawara et al, 2007], proapoptotic [Mertens-Talcott & Percival, 2005], antiproliferative [Letchoumy et al, 2007], antiangiogenic [Lamy et al, 2006], anti-infl ammatory [Clavin et al, 2007]. More precisely, they can infl uence anti-apoptotic proteins (e.g.…”

Section: Introductionmentioning

confidence: 99%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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Key words: evening primrose, matrix metalloproteinases, cancer Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid --linolenic acid (GLA) -but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the infl uence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.The results revealed that the examined polyphenol extract had little infl uence on Caco-2 proliferation, but effectively in a time-and dose-dependent manner inhibited MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF- and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 mol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.

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Anti-inflammatory activity of flavonoids from Eupatorium arnottianum

Cited by 119 publications

References 21 publications

Comparison of the Antiinflammatory Activities of Three Medicinal Plants Known as “Meiduoluomi” in Tibetan Folk Medicine

Comparison of the Antiinflammatory Activities of Three Medicinal Plants Known as “Meiduoluomi” in Tibetan Folk Medicine

Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

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