Anti-inflammatory activity of flavonoids fromPopulus davidiana

Zhang, Xinfeng; Hung, Tran Manh; Phuong, Phuong Thien; Ngoc, Tran Minh; Min, Byung‐Sun; Song, Kyung‐Sik; Seong, Yeon Hee; Bae, KiHwan

doi:10.1007/bf02969299

Cited by 135 publications

(106 citation statements)

References 23 publications

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“…15,16) We chose KM due to its beneficial effects such as an antioxidant, anti-inflammatory, anti-hypertensive, and is an anti-carciogenic agent. [17][18][19][20] It has been shown that KM can provide maximum attenuation of neuronal diseases progression in ROS-mediated cellular toxicity in both in vitro and in vivo. 17,21) Nuclear factor-kappa B (NF-κB) is a redox-sensitive transcription factor that conduct different physiological event involving inflammatory process, cellular development, and apoptosis.…”

Section: )mentioning

confidence: 99%

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells via Modulating Expression of MUC5AC

Podder

Song

et al. 2014

Biological & Pharmaceutical Bulletin

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Mucins are highly glycosylated secretary proteins produced by most epithelial cells. Hypersecretion of mucins is one of the prominent symptoms of several airway diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. Paraquat (PQ), a common herbicide, has been associated with pulmonary damage and is a potent reactive oxygen species (ROS) producer. However, until now the role of PQ on mucin overproduction has not been studied. The aim of this study is to explore how kaempferol (KM), a widely used dietary flavonoid, affects the protection of human PQ-exposed bronchial epithelium BEAS-2B cells by suppressing Mucin gene expression via nuclear factor-kappa B (NF-κB). We observed that PQ generates intracellular ROS, and also induces lipid peroxidation in BEAS-2B cells. Additionally, we found that PQ effectively induces the expression of the MUC5AC gene; however, co-treatment of PQ with KM drastically reduces its expression. Furthermore, we observed that PQ activates NF-κB, while co-treatment with KM occludes its nuclear translocation, and additionally KM repressed the PQ phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in BEAS-2B cells. Based on our data, we believe that KM can suppress the over-expression of the MUC5AC gene. This would contribute to the protection of PQ cytotoxicity to exposed BEAS-2B cells, and allow further study toward a better understanding of ROSassociated diseases.Key words MUC5AC; kaempferol; reactive oxygen species; human bronchial epithelial BEAS-2B cell; nuclear factor-kappa B Several studies have been carried out demonstrating hypersecretion of mucous from respiratory epithelial cells a major pathological event in chronic airway diseases such as chronic obstructive pulmonary diseases or asthma.1,2) These pathological manifestations are governed by the interaction between underlying genetic factors and xenobiotics, which together are responsible for intercellular reactive oxygen species (ROS) production.3) Findings have shown that ROS such as: hydrogen peroxide, superoxide anion, hydroxyl ion are responsible for the initiation and progression of chronic airway diseases via over-expression of Mucin genes.4-6) ROS is a major etiological factor in the pathogenesis of chronic inflammatory airway diseases as it triggers mucin secretion in epithelia. 7)Twenty different MUC genes have been characterized in human airway epithelia, these are then subdivided into membrane-bound and secreted forms.8-11) MUC5AC is a hallmark gene, and considered the most phenotypical gene in respiratory diseases; it accumulates in respiratory tract can lead to symptoms such as chronic cough and excessive sputum production. Previous studies showed that paraquat (PQ) is a potent ROS inducer in different epithelial cell lines [12][13][14] and is associated with cellular cytoxicity. However, there has been no study into whether PQ can induce MUC gene expression or not, so in this study we investigated the effects of PQ on Bronchial epithelial BEAS...

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Section: )mentioning

confidence: 99%

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells via Modulating Expression of MUC5AC

Podder

Song

et al. 2014

Biological & Pharmaceutical Bulletin

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“…In the HMBC spectrum of 4, correlation of methoxyl proton (δ 3.81, s), H-3' (δ 6.34, s), and H-5' (δ 6.15, s) with C-4' (δ 166.19 ) was observed. HMBC correlation of the anomeric proton (δ 5.17, d, J =7.5 Hz, H-1") with the carbon at δ 159.81 (C-2') indicated the presence of sugar moiety at C-2' position [10,11]. According to the above data comparison with the literature [11,12], the compound 4 was determined as neosakuranin, a chalcone glucoside, and reported for the first time in domestic from the outer bark of P. sargentii.…”

Section: Resultsmentioning

confidence: 99%

Antioxidative Activity of Prunus sargentii Outer Bark Extractives

Park

Bae

2012

Journal of the Korean Wood Science and Technology

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The outer bark of Prunus sargentii was collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and H2O. From the EtOAc soluble fraction, four compounds were isolated by the repeated Sephadex LH-20 column chromatography. The isolated compounds were determined as (+)-catechin (1), (-)-epicatechin (2), taxifolin (3), and neosakuranin (4) by the spectroscopic analysis including 1 H, 13C-NMR, and 2D-NMR spectrometers. The antioxidative activities on the isolated compounds and the separated fractions were evaluated by DPPH radical scavenging assay. The crude, EtOAc, and H2O soluble fractions indicated good antioxidative potential compared to the CH2Cl2 and n-hexane soluble fractions.

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“…Sorbus commixta (S. commixta) cortex has long been used in the field of traditional Oriental medicine as a tonic to treat coughing, asthma, and other bronchial disorders (Bae et al, 2000). From the cortex of S. commixta cortex, triterpenoids such as lupenone and lupeol have been isolated Zhang et al, 2006). Recently, the methanol extract of S. commixta cortex (MSC) was shown to have a potent radical scavenging activity (Na et al, 2002).…”

Section: Mgmentioning

confidence: 99%

Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase

You¹,

Kim²,

Bhatt³

et al. 2010

Oriental Pharmacy and Experimental Medicine

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SUMMARYAn examination of the kinetic properties of UDP-glucose, (1,3)-β-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-β-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

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Anti-inflammatory activity of flavonoids fromPopulus davidiana

Cited by 135 publications

References 23 publications

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells <i>via</i> Modulating Expression of <i>MUC5AC</i>

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells <i>via</i> Modulating Expression of <i>MUC5AC</i>

Antioxidative Activity of Prunus sargentii Outer Bark Extractives

Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase

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