Anti-Inflammatory Activity of N-Butanol Extract from Ipomoea stolonifera In Vivo and In Vitro

Abstract

Ipomoea stolonifera (I. stolonifera) has been used for the treatment of inflammatory diseases including rheumatism and rheumatoid arthritis in Chinese traditional medicine. However, the anti-inflammatory activity of I. stolonifera has not been elucidated. For this reason, the anti-inflammatory activity of n-butanol extract of I. stolonifera (BE-IS) was evaluated in vivo by using acute models (croton oil-induced mouse ear edema, carrageenan-induced rat paw edema, and carrageenan-induced rat pleurisy) and chronic models (cotton pellet-induced rat granuloma, and complete Freund’s adjuvant (CFA)-induced rat arthritis). Results indicated that oral administration of BE-IS significantly attenuated croton oil-induced ear edema, decreased carrageenan-induced paw edema, reduced carrageenan-induced exudates and cellular migration, inhibited cotton pellet-induced granuloma formation and improved CFA-induced arthritis. Preliminary mechanism studies demonstrated that BE-IS decreased the levels of myeloperoxidase (MPO) and malondialdehyde (MDA), increased the activity of anti-oxidant enzyme superoxide dismutase (SOD) in vivo, and reduced the production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in lipopolysaccharide-activated RAW264.7 macrophages in vitro. Results obtained in vivo and in vitro demonstrate that BE-IS has considerable anti-inflammatory potential, which provided experimental evidences for the traditional application of Ipomoea stolonifera in inflammatory diseases.

Pharmacological, phytochemical and morphological study of three Mayan accessions of Bixa orellana L. leaves

“…Inflammation was induced in the right paw by a sub-plantar injection of 0.1 mL of carrageenan 1% (w/v) in saline solution 0.9% (w/v) (Cai et al, 2014). The change in edema was calculated according to the difference in the measured diameter between the non-swollen left paw and right paw treated with carrageenan.…”

Anti-inflammatory activity of the essential oils of Cymbopogon validus (Stapf) Stapf ex Burtt Davy from Eastern Cape, South Africa

“…Baseline right hind paw diameter (paw size) was determined for each rat using a pair of YATO digital caliper [14][15][16][17]. Rats were administered with oral doses of drugs as per assigned group.…”

Anti-inflammatory activity and phenolic composition of Dendropanax morbifera leaf extracts

“…A variety of steroidal or non-steroidal anti-inflammatory drugs have been developed and used to treat inflammatory diseases. However, prolonged use of these drugs can cause many side effects, including immunodeficiency, gastrointestinal disorders, and humoral disturbances (Simon, 2003;Roth, 2012;Cai et al, 2014). The discovery of new anti-inflammatory agents with few side effects that could be developed for long-term administration remains an outstanding challenge.…”