Anti-inflammatory activity of Protium spruceanum (Benth.) Engler is associated to immunomodulation and enzymes inhibition

Amparo, Tatiane Roquete; Seibert, Janaína Brandão; Mathias, Fernando Augusto Siqueira; Vieira, João Filipe Pereira; Aguiar-Soares, Rodrigo Dian de Oliveira; Freitas, Kátia Michelle; Cabral, Vivette Appolinário Rodrigues; Brandão, Geraldo Célio; Santos, Orlando David Henrique dos; Souza, Gustavo Henrique Bianco de; Vieira, Paula Melo de Abreu

doi:10.1016/j.jep.2019.112024

Cited by 12 publications

(14 citation statements)

References 49 publications

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“…Meanwhile, compounds 4, 6, 13, and 14 suppressed the NF-κB signaling pathways to limit the expression of COX-2 (25-28). Differently, compounds 7, 10, 17, 18, and 21 were proven to reduce COX-2 levels by reducing nitric oxide overproduction from inducible nitric oxide synthase (29)(30)(31). Considering the aforementioned facts, we can still conclude that, the compounds analyzed by UPLC-Q-Exactive Orbitrap MS/MS were consistent.…”

Section: Evaluate Of Cox-2 Inhibitors In Choerospondias Axillarismentioning

confidence: 81%

Magnetic Ligand Fishing Using Immobilized Cyclooxygenase-2 for Identification and Screening of Anticoronary Heart Disease Ligands From Choerospondias axillaris

Miaomiao¹,

Wang²,

Yan³

et al. 2022

Front. Nutr.

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Inhibition of cyclooxygenase-2 (COX-2) activity is an effective way for treatment of coronary heart disease. And as an important source of COX-2 inhibitors, bioactive compounds of Choerospondias axillaris and pharmacological mechanisms remained lacking in prospective researches. Therefore, for the purpose of accelerating the discovery of natural products targeting designed inhibitors, the COX-2 microreactor composed of functionalized microspheres and magnetic ligand fishing was developed and applied in Choerospondias axillaris, and the physicochemical properties of the COX-2 functionalized microspheres were characterized using Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Furthermore, the bioactive compounds singled out from ethanol decoction without prepurification were dissociated and identified by ultraperformance liquid chromatography plus Q-Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS/MS). Consequently, 21 bioactive compounds consisting of 6 organic acids, 8 flavonoids, and 7 others were separated and characterized from Choerospondias axillaris, which were reported to participate in the COX-2 inhibitory pathway to varying degrees. Therefore, this method could provide a prospective solution for the extraction and identification of active pharmaceutical ingredients and the rapid screening of some enzyme inhibitors in the complex mixtures.

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Section: Evaluate Of Cox-2 Inhibitors In Choerospondias Axillarismentioning

confidence: 81%

Magnetic Ligand Fishing Using Immobilized Cyclooxygenase-2 for Identification and Screening of Anticoronary Heart Disease Ligands From Choerospondias axillaris

Miaomiao¹,

Wang²,

Yan³

et al. 2022

Front. Nutr.

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“…Hyperoside also has the ability to inhibit inflammasome activation [38] and Nrf2 [39], which could collective explain its anti-inflammatory profile. Portulaca olearacea [40] and Protium spruceanum [41], both rich in quercitrin, proved to suppress NF-kB/activate MAPK and exerted immunomodulation/enzyme activation, which could lead to their anti-inflammatory effect. Finally, the anti-inflammatory action of Myracrodruon urundeuva and Tetraclinis articulata is correlated to the presence of afzelin [42,43].…”

Section: Discussionmentioning

confidence: 99%

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

et al. 2019

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Crataegus almaatensis, an endemic ornamental plant in Kazakhstan is used in popular medicine due to its cardiotonic properties. The most studied species of the same genus are commonly found in Europe, which shows the importance of having the Kazakh species validated via its chemical and pharmacological studies. High-speed countercurrent chromatography (HSCCC) operated under optimized conditions enabled an isolation of the three main compounds from the aqueous phase of the leaves ethanol extract, further identified by nuclear magnetic resonance (NMR), as quercetin 3-O-rhamnoside (quercitrin) (4.02% of the crude extract-CECa); quercetin 3-O-β-galactoside (hyperoside) (1.82% of CECa); kaempferol 3-O-α-L-rhamnoside (afzelin) (0.94% of CECa). The CECa, the aqueous phase of the crude extract (APCa) together with the isolates were evaluated for their vascular (vascular reactivity in human internal mammary artery-HIMA), anti-nociceptive (formalin-induced liking response and hot plate) and anti-inflammatory (subcutaneous air-pouch model-SAP) activities. CECa at the concentrations of 0.014 and 0.14 mg/mL significantly increased the maximum contractility response of HIMA to noradrenaline. The APCa CR curve (0.007–0.7 mg/mL) showed an intrinsic relaxation effect of the HIMA. APCa at the dose of 100 mg/kg i.p. significantly decreased the total leukocyte count and the IL-1β release in the SAP wash.

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“…NO production was assessed indirectly by the quantification of nitrite and nitrate according to the Griess reaction [62,63]. DEX was selected as a positive control [64].…”

Section: Methodsmentioning

confidence: 99%

Antitumor, Antiviral, and Anti-Inflammatory Efficacy of Essential Oils from Atractylodes macrocephala Koidz. Produced with Different Processing Methods

Huang

et al. 2019

Molecules

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Atractylodes macrocephala Koidz. has been used as an invigorating spleen drug for eliminating dampness and phlegm in China. According to recent researches, different processing methods may affect the drug efficacy, so we collected A. macrocephala from the Zhejiang Province, produced with different processing methods, crude A. macrocephala (CA) and bran-processed A. macrocephala (BA), then analyzed its essential oils (EOs) by GC/MS. The results showed 34 components representing 98.44% of the total EOs of CA were identified, and 46 components representing 98.02% of the total EOs of BA were identified. Atractylone is the main component in A. macrocephala. Compared with CA, BA has 46 detected compounds, 28 of which were identical, and 6 undetected compounds. Pharmacodynamic results revealed that the EOs of CA and atractylone exhibited more effective anticancer activity in HepG2, MCG803, and HCT-116 cells than the EOs of BA; while the EOs of BA exhibited simple antiviral effect on viruses H3N2, both the EOs and atractylone show anti-inflammatory activity by inhibiting the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in ANA-1 cells.

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Anti-inflammatory activity of Protium spruceanum (Benth.) Engler is associated to immunomodulation and enzymes inhibition

Cited by 12 publications

References 49 publications

Magnetic Ligand Fishing Using Immobilized Cyclooxygenase-2 for Identification and Screening of Anticoronary Heart Disease Ligands From Choerospondias axillaris

Magnetic Ligand Fishing Using Immobilized Cyclooxygenase-2 for Identification and Screening of Anticoronary Heart Disease Ligands From Choerospondias axillaris

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

Antitumor, Antiviral, and Anti-Inflammatory Efficacy of Essential Oils from Atractylodes macrocephala Koidz. Produced with Different Processing Methods

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