Anti-inflammatory activity of Sorbus commixta water extract and its molecular inhibitory mechanism

Yu, Tao; Lee, Yong Jin; Jang, Hyun‐Jae; Kim, Ae Ra; Hong, Sungyoul; Kim, Tae‐Woong; Kim, Mi‐Yeon; Lee, Jaehwi; Lee, Yong Gyu; Cho, Jae Youl

doi:10.1016/j.jep.2010.12.032

Cited by 70 publications

(52 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[47][48][49][50] Recent studies have reported that they possess antioxidant and anti-inflammatory properties. 33,34,[48][49][50][51][52][53][54] Oxidative damage and neuroinflammation are fundamental to the progression of AD; therefore, these plants would provide neuroprotective activity through their antioxidative and anti-inflammatory functions. Supporting this idea, we found that S. commixta intake significantly reduced ROS levels as well as rescuing the AD-like phenotypes in Drosophila.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using Drosophila Models of Alzheimer’s Disease

Liu

Lee

Kim

et al. 2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Alzheimer's disease (AD) is the most common neurodegenerative disorder, characterized by progressive neuronal loss with amyloid β-peptide (Aβ) plaques. Despite several drugs currently used to treat AD, their beneficial effects on AD progress remains under debate. Here, we established a rapid in vivo screening system using Drosophila AD models to assess the neuroprotective activities of medicinal plants that have been used in traditional Chinese medicine. Among 23 medicinal plants tested, the extracts from five plants, Coriandrum sativum, Nardostachys jatamansi, Polygonum multiflorum (P. multiflorum), Rehmannia glutinosa, and Sorbus commixta (S. commixta), showed protective effects against the Aβ42 neurotoxicity. We further characterized the neuroprotective activity of ethanol extracts from P. multiflorum and S. commixta. Aβ42-expressing flies that we used showed AD neurological phenotypes, such as decreased survival and motility and increased cell death and reactive oxygen species level. However, feeding these flies extracts from P. multiflorum or S. commixta showed strong suppression of such phenotypes. Similar results were observed in human cells, so that the treatment of P. multiflorum and S. commixta extracts increased the viability of Aβ-treated SH-SY5Y cells. Moreover, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, one of the main constituents of P. multiflorum, also showed similar protective activity against Aβ42 cytotoxicity in both Drosophila and human cells. Taken together, our results suggest that both P. multiflorum and S. commixta have therapeutic potential for the treatment of neurodegenerative diseases, such as AD.Key words amyloid β-peptide 42 (Aβ42); Alzheimer's disease; Drosophila; Polygonum multiflorum; reactive oxygen species (ROS); Sorbus commixta Alzheimer's disease (AD) is a common neurodegenerative disease that is characterized by progressive neural loss caused by amyloid β-peptide (Aβ) plaques and cognitive deficits. 1,2) During disease progression, neurotoxic forms of Aβ cause neuronal damages via various cellular abnormalities such as increased oxidative damage, impaired energy metabolism, disrupted cellular calcium homeostasis, and increased inflammatory response.2,3) Several drugs have been approved to treat AD, given the dysfunction of cholinergic and glutamatergic neurotransmission; they function as acetylcholinesterase inhibitors or N-methyl-D-aspartate (NMDA) receptor antagonists. 4,5) However, the beneficial effects of these drugs during the progression of AD are still in question. 6) Due to its complex etiology, a combination therapy including the use of medicinal plants with effective components has been proposed as an alternative choice for treatment of AD. 7,8) Chinese traditional medicine has employed a variety of herbs to treat dementia, and a number of neuroprotective agents were isolated from these herbs. 7,8) For example, Polygonum multiflorum (P. multiflorum; syn. Reynoutria multiflora) has been known as a tonic and antiaging agent in many remedies for centuries, and...

show abstract

Section: Discussionmentioning

confidence: 99%

In Vivo Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using Drosophila Models of Alzheimer’s Disease

Liu

Lee

Kim

et al. 2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…HAD-B-mediated suppression of LPS-induced inflammatory responses with IC 50 values of 115 (NO) and 156 (PGE 2 ) lg/mL was not strong, but comparable to those of other medicinal plants, such as Phaseolus calcaratus, Sorbus commixta, Sanguisorba officinalis, Acer tegmentosum, Hibiscus cannabinus, and Cinnamomum camphora, with IC 50 values ranging from 100 to 300 lg/mL. [34][35][36][37][38] These results indicate that the inhibitory activity of HAD-B is derived from its ability to block LPS/ TLR4-mediated inflammatory responses at the transcriptional level regulated by NF-jB and ATF-2.…”

Section: Resultsmentioning

confidence: 62%

“…Recent studies have shown that several ethnomedicinal herbs, such as C. militaris, Kaempferia parviflora, S. commixta, include unidentified compounds that act directly as Syk inhibitors. 36,43 Critical roles of Syk and Src in LPS/TLR4-mediated inflammatory pathways have been established using specific inhibitors, such as BAY61-3606, piceatannol, and PP2. For example, piceatannol is able to block the production of proinflammatory cytokines.…”

Section: Fig 1 Effect Of Had-b On Inflammatory Responses In Vitro mentioning

confidence: 99%

HangAmDan-B, an Ethnomedicinal Herbal Mixture, Suppresses Inflammatory Responses by Inhibiting Syk/NF-κB and JNK/ATF-2 Pathways

Moh

Kim

et al. 2013

Journal of Medicinal Food

Self Cite

View full text Add to dashboard Cite

HangAmDan-B (HAD-B) is a powdered mixture of eight ethnopharmacologically characterized folk medicines that is prescribed for solid masses and cancers in Korea. In view of the finding that macrophage-mediated inflammation is a pathophysiologically important phenomenon, we investigated whether HAD-B modulates inflammatory responses and explored the associated molecular mechanisms. The immunomodulatory activity of HAD-B in toll-like receptor-activated macrophages induced by lipopolysaccharide (LPS) was assessed by measuring nitric oxide (NO) and prostaglandin E2 (PGE2) levels. To identify the specific transcription factors (such as nuclear factor [NF]-jB and signaling enzymes) targeted by HAD-B, biochemical approaches, including kinase assays and immunoblot analysis, were additionally employed. HAD-B suppressed the production of PGE2 and NO in LPS-activated macrophages in a dose-dependent manner. Furthermore, the extract ameliorated HCl/EtOH-induced gastritis symptoms. Moreover, HAD-B significantly inhibited LPS-induced mRNA expression of inducible NO synthase and cyclooxygenase (COX)-2. Interestingly, marked inhibition of NF-jB and activating transcription factor was observed in the presence of HAD-B. Data from direct kinase assays and immunoblot analysis showed that HAD-B suppresses activation of the upstream signaling cascade involving spleen tyrosine kinase, Src, p38, c-Jun N-terminal kinase, and transforming growth factor b-activated kinase 1. Finally, kaempferol, but not quercetin or resveratrol was identified as a bioactive compound in HAD-B. Therefore, our results suggest that HAD-B possesses anti-inflammatory activity that contributes to its anticancer property.

show abstract

“…LY294002 (LY), BAY11-7082 (BAY), U0126, and wortmannin were from Calbiochem (La Jolla, CA, USA). Luciferase constructs containing binding promoters for NF-κB and AP-1 were used as reported previously [12,13] . Enzyme immunoassay (EIA) kits and enzyme-linked immunosorbent assay (ELISA) kits for PGE 2 and TNF-α were purchased from Amersham (Little Chalfont, Buckinghamshire, UK).…”

Section: Methodsmentioning

confidence: 99%

8-(Tosylamino)quinoline inhibits macrophage-mediated inflammation by suppressing NF-κB signaling

et al. 2012

Self Cite

View full text Add to dashboard Cite

Aim:The macrophage-mediated inflammatory response may contribute to the development of cancer, diabetes, atherosclerosis and septic shock. This study was to characterize several new compounds to suppress macrophage-mediated inflammation. Methods: Peritoneal macrophages from C57BL/6 male mice and RAW264.7 cells were examined. Anti-inflammatory activity was evaluated in the cells exposed to lipopolysaccharide (LPS). The mechanisms of the anti-inflammatory activity were investigated via measuring transcription factor activation in response to specific signals and via assaying the activities of the target kinases. Results: Of 7 candidate compounds tested, 8-(tosylamino)quinoline (8-TQ, compound 7) exhibited the strongest activities in suppressing the production of NO, TNF-α, and PGE 2 in LPS-activated RAW264.7 cells and peritoneal macrophages (the IC 50 values=1-5 μmol/L). This compound (1.25-20 μmol/L) dose-dependently suppressed the expression of the pro-inflammatory genes for iNOS, COX-2, TNF-α, and the cytokines IL-1β and IL-6 at the level of transcription in LPS-activated RAW264.7 cells. 8-TQ (20 μmol/L) significantly suppressed the activation of NF-κB and its upstream signaling elements, including inhibitor of κB (IκBα), IκBα kinase (IKK) and Akt in LPSactivated RAW264.7 cells. In in vivo experiments, oral administration of 20 and 40 mg/kg 8-TQ for 3 d significantly alleviated the signs of LPS-induced hepatitis and HCl/EtOH-induced gastritis, respectively, in ICR mice. Conclusion: 8-TQ (compound 7) exerts significant anti-inflammatory activity through the inhibition of the Akt/NF-κB pathway, thus may be developed as a novel anti-inflammatory drug.

show abstract

Anti-inflammatory activity of Sorbus commixta water extract and its molecular inhibitory mechanism

Cited by 70 publications

References 43 publications

<i>In Vivo</i> Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using <i>Drosophila</i> Models of Alzheimer’s Disease

<i>In Vivo</i> Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using <i>Drosophila</i> Models of Alzheimer’s Disease

HangAmDan-B, an Ethnomedicinal Herbal Mixture, Suppresses Inflammatory Responses by Inhibiting Syk/NF-κB and JNK/ATF-2 Pathways

8-(Tosylamino)quinoline inhibits macrophage-mediated inflammation by suppressing NF-κB signaling

Contact Info

Product

Resources

About