Anti-inflammatory Activity of Sting Protein from Apis mellifera

Abstract

ABSTRACT-The objective of our study is to determine its anti-inflammatory potential of protein extracted from the stings of honey bee (Apis mellifera). In this study, protein extracted from the stings of Apis mellifera using Tris HCl/ice cold acetone and determined through Nano drop method and then determined its Da protein using SDS-PAGE. In addition, indirect ELISA was performed using rubella vaccine as coating antigen and determined its antibody titre using variable concentration of sting protein (15.62-250 µg) and also determined its activity on human whole blood for determining total cellular content and proliferation against rubella vaccine antigen. The results showed that protein from stings of Apis mellifera showed drastic declined in antibody titre at higher doses but there is slightly enhancement in antibody titre, total cellular content and proliferations at lower concentration as compared to control and rubella vaccine (standard).Overall, this study suggest that stings protein of Apis mellifera showed anti-inflammatory potential against rubella vaccine antigen.

Antimicrobial Properties of Apis mellifera’s Bee Venom

“…Another promising and feasible implication is to test BV to combat microbes causing skin diseases. Interestingly, BV can be useful as a topical agent for encouraging skin regeneration or treatment of certain epidermal conditions [5,94]. Therefore, BV has contributed to some formulations against bacteria that cause acne [95,96].…”

Zooceuticals and Cosmetic Ingredients Derived from Animals

“…It is also known as apitoxin and is used in cosmetics as an astringent and for its antiinflammatory, anti-ageing [27], and anti-bacterial [28] properties. It represents a new entry into the cosmetic field, particularly for mature skin.…”

Cosmetic Applications of Bee Venom

“…Accordingly, the BV remedy has been developed to deal with various sicknesses, including inflammation, cancers, microbial diseases, and neurodegenerative diseases [1][2][3][4]. Recently, BV has been used as a topical treatment for atopic dermatitis and acne because of its anti-inflammatory, anti-ageing [5,6], and anti-bacterial properties [7]. BV can be useful as a topical agent for encouraging skin regeneration and thus treatment of certain epidermal conditions [6,7].…”

“…Recently, BV has been used as a topical treatment for atopic dermatitis and acne because of its anti-inflammatory, anti-ageing [5,6], and anti-bacterial properties [7]. BV can be useful as a topical agent for encouraging skin regeneration and thus treatment of certain epidermal conditions [6,7]. BV's topical administration can be well tolerated in human skin as it has shown no risk for dermal irritation in animal studies [8].…”