Anti-inflammatory activity of the major constituents ofLonicera japonica

Lee, Song Jin; Shin, Eun Joo; Son, Kun Ho; Chang, Hyeun Wook; Kang, Sam Sik; Kim, Hyun Pyo

doi:10.1007/bf02979147

Cited by 70 publications

(38 citation statements)

References 6 publications

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“…Ms could inhibit the mouse ear edema provoked by croton oil and also the carrageenan-induced hind paw edema in rats. Following this, loniceroside A and loniceroside C, isolated from Lonicera japonica, were shown to possess anti-inflammatory activity in a croton oil-induced ear edema model in vivo (11,12). In the present study, lonimacranthoide VI was demonstrated to inhibit the LPS-induced production of PGE 2 , indicating anti-inflammatory effects.…”

Section: Discussionsupporting

confidence: 61%

Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin

Guan

Wang

et al. 2014

Biomedical Reports

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Abstract. Lonimacranthoide VI, first isolated from the flower buds of Lonicera macranthoides in our previous study, is a rare chlorogenic acid ester acylated at C-23 of hederagenin. In the present study, the anti-inflammatory effects of lonimacranthoide VI were studied. Lipopolysaccharides (LPS) induced an inflammatory response through the production of prostaglandin E 2 (PGE 2 ), and these levels were reduced when lonimacranthoide VI was pre-administered. Additionally, the mechanism of the anti-inflammatory effects of lonimacranthoide VI was investigated by measuring cyclooxygenase (COX) activity and mRNA expression. The results showed that lonimacranthoide VI inhibited mRNA expression and in vitro activity of COX-2 in a dose-dependent manner, whereas only the higher lonimacranthoide VI concentration possibly reduced COX-1 expression and in vitro activity. Taken together, these results indicate that lonimacranthoide VI is an important anti-inflammatory constituent of Lonicera macranthoides and that the anti-inflammatory effect is attributed to the inhibition of PGE 2 production through COX activity and mRNA expression.

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Section: Discussionsupporting

confidence: 61%

Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin

Guan

Wang

et al. 2014

Biomedical Reports

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“…Iridoids such as loganin have been isolated from these plant materials as major constituents. These compounds have also been reported to show anti-infl ammatory activity (Lee et al, 1995). All of this background information led us to examine the inhibitory activity of BL against lung infl ammation, and the results of the present investigation demonstrated that BL is effective for the treatment of animal models of lung infl ammation at doses of 200-400 mg/kg.…”

Section: Discussionmentioning

confidence: 53%

Inhibition of Experimental Lung Inflammation and Bronchitis by Phytoformula Containing Broussonetia papyrifera and Lonicera japonica

Ko¹,

Jin²,

Kwon³

et al. 2011

Biomolecules and Therapeutics

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Many patients suffer from lung infl ammatory disorders including asthma, bronchitis and chronic obstructive pulmonary disorders (COPD). Several classes of drugs including bronchodilators, antitussives, steroids and leukotriene (LT) receptor antagonists have been used to treat these disorders. However, these drugs sometimes do not provide fundamental care, even though the major symptoms are alleviated (Jeffery, 2001). Thus, new effective drugs with novel action mechanism(s) are needed to treat lung infl ammatory disorders. In this regard, natural products may be applied safely for treating human lung infl ammation and bronchitis.The root barks of Broussonetia papyrifera (L.) Vent. (Moraceae) distributed in China, Japan and Korea have been used as anti-infl ammatory and antitussive agents in traditional Chinese medicine, especially for the treatment of older people having bronchitis (Editorial Committee, 1999). Recently, the 95% ethanol extracts of the radix, stems, leaves and fruits of B. papyrifera showed antinociceptive and anti-infl ammatory activity in vivo, with radix having the strongest activity (Lin et al., 2008). Lonicera japonica (Thunb.) (Caprifoliaceae) is a Broussonetia papyrifera and Lonicera japonica have long been used in the treatment of infl ammatory disorders, especially respiratory infl ammation, in Chinese medicine. Previously, phytoformula (BL) containing B. papyrifera and L. japonica was found to exert strong anti-infl ammatory activity in vitro and in vivo. In this study, the effects of BL on lung infl ammation including bronchitis were examined in vitro and in vivo. BL (10-100 μg/ml) inhibited nitric oxide (NO) production of lipopolysaccharide (LPS)-treated alveolar macrophages, MH-S cells, primarily by down-regulating inducible NO synthase. BL also inhibited production of the proinfl ammatory cytokines, TNF-α and IL-6. Against an animal model of pleural cavity infl ammation, BL (200-400 mg/kg) signifi cantly inhibited 5 h and 24 h carrageenan-induced pleurisy in rats when administered orally. Additionally, BL inhibited experimental bronchitis induced by intratracheal instillation of LPS to rats. Taken together, these results indicate that BL may be effective for the treatment of human lung infl ammation as well as bronchitis.

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“…The exact reason for these enhancing effects of BL is not known yet; however, it is reasonably speculated that the constituents in both extracts act synergistically. The major constituents in EBP are prenylated flavonoids and the majors of ELJ are hederagenin and iridoid derivatives (Lee et al, 1995;Son et al, 2001;Kwak et al, 2003a). These constituents may synergistically and cooperatively act at multiple points (5-LOX, COX-2, iNOS, etc.)…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Lonicera japonica has been used as an anti-inflammatory agent in Korea since ancient times and is widely used for the treatment of upper respiratory tract infections, diabetes mellitus, and rheumatoid arthritis (Lee et al, 1998). Various constituents have been isolated from the total plant materials, including lonicerosides and iridoids, and their anti-inflammatory activities have been evaluated (Lee et al, 1995;Kwak et al, 2003a;Qian et al, 2007). To develop a new anti-inflammatory herbal drug, the ethanol extract (ELJ) was examined for its in vitro and in vivo activity in animal models of inflammation.…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Activity of the Total Flavonoid Fraction from Broussonetia papyrifera in Combination with Lonicera japonica

Jin¹,

Lim²,

Kwon³

et al. 2010

Biomolecules and Therapeutics

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-To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed PGE2 and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against λ-carrageenaninduced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.

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Anti-inflammatory activity of the major constituents ofLonicera japonica

Cited by 70 publications

References 6 publications

Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin

Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin

Inhibition of Experimental Lung Inflammation and Bronchitis by Phytoformula Containing Broussonetia papyrifera and Lonicera japonica

Anti-Inflammatory Activity of the Total Flavonoid Fraction from Broussonetia papyrifera in Combination with Lonicera japonica

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