Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii

Oliveira, Arthur Henrique Cavalcante de; Oliveira, Gibson Gomes de; Neto, Fausto Carnevale; Portuondo, Deivys Leandro; Batista-Duharte, Alexander; Carlos, Iracilda Zeppone

doi:10.1016/j.jep.2016.11.030

Cited by 22 publications

(15 citation statements)

References 48 publications

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“…Generally, the treatment of fungal infections with ITR may be due not only to its direct antifungal effect, but also due to the induction of an immunomodulatory effect on the host defense miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis systems via the regulation of cytokine production (12). ITR has an anti-inflammatory effect in vivo and in vitro (13,14). In human lymphoid cells, ITR suppresses the accumulation of TNF-α and IFN-γ messenger RNAs (mRNAs) in the presence of polyhydroxyalkanoate (15).…”

Section: Introductionmentioning

confidence: 99%

miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis

Zhang

et al. 2018

Int J Mol Med

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Previous studies have indicated that monocyte chemoattractant protein-1 (MCP‑1), also referred to as C‑C motif chemokine ligand 2, has a significant role in the pathogenesis of sepsis, however, how microRNAs (miRs) contribute to this process remains to be fully elucidated. In the present study, using a mouse model of disseminated candidiasis, the renoprotective effect of itraconazole (ITR) and adenovirus‑delivered miR‑124 was investigated. The mice were treated with ITR (50 mg/kg) or transfected with miR‑124 mimics via tail‑vein injection 7 days prior to Candida albicans infection. The survival outcome was monitored following candidiasis‑induced sepsis with ITR or miR‑124 mimics treatment. The levels of pro‑inflammatory cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β) and IL‑6, were determined using enzyme‑linked immunosorbent assays. The mRNA and protein levels were assayed using reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. The results showed that ITR and miR‑124 mimics improved the survival outcome in candidiasis‑induced septic mice. The findings also indicated a significant downregulation in the serum levels of TNF‑α, IL‑1β and IL‑6 in the septic mice treated with ITR or miR‑124 mimics. Of note, ITR treatment significantly increased the expression of miR‑124 and decreased the levels of MCP‑1 in the kidneys of the septic mice. It was also shown that the overexpression of miR‑124 reduced the expression of MCP‑1 and attenuated candidiasis‑induced acute kidney injury (AKI) in septic mice. Transfection with miR‑124 mimics was equivalent to ITR in reducing the excessive inflammatory response and renal lesions in septic mice. These results provided evidence supporting the use of miR‑124 mimics as a therapeutic approach for attenuating candidiasis-induced AKI.

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Section: Introductionmentioning

confidence: 99%

miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis

Zhang

et al. 2018

Int J Mol Med

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“…Así mismo, los taninos se detectaron en la fracción de metanol y la fracción soluble en acetona dio positivo el ensayo para antraquinonas (Tabla 1). Estudios fitoquímicos realizados sobre especies del género Vismia, revelan que este género es una buena fuente de xantonas, antraquinonas, terpenos, esteroles, polifenoles y flavonoides; este hecho es relevante, debido a que este estudio reveló que el extracto de la especie Vismia cayennensis, colectada en latitudes venezolanas, posee en su composición química, muchas de estas familias de metabolitos secundarios con actividades biológicas (Singh et al, 2005;Nguemeving et al, 2006;Epifano et al, 2007;Noungoue et al, 2007;Yadav et al, 2016;Buitrago et al, 2016;Oliveira et al, 2017).…”

Section: Resultados Y Discusión 31 Análisis Fítoquímicounclassified

Estudio fitoquímico y biológico preliminar de la corteza (tallo) de Vismia cayennensis proveniente del estado Amazonas, Venezuela.// Phytochemical and biological preliminary study of Vismia cayennensis bark (steam) from Amazonas state, Venezuela

Marín¹,

Regnault

Maillo³

et al. 2017

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ResumenSe realizó un estudio químico y biológico preliminar de la especie Vismia cayennensis, colectada en el estado Amazonas, Venezuela. El ensayo antibacteriano del extracto de la corteza de la planta reveló una inhibición significativa de los microorganismos Escherichia coli, Shigella sp. y Staphylococcus aureus. Además, tres de las cincos fracciones solubles en solventes de distintas polaridades, específicamente las de cloroformo, acetona y agua, mantuvieron de manera moderada, esta actividad frente a la cepa Shigella sp. El extracto hidroalcohólico de la planta y la fracción soluble en cloroformo, exhibieron un efecto antiinflamatorio apreciable. Los ensayos de citotoxicidad realizados por los métodos de (3-(4,5-dimetiltiazol-2-il)-5-(3-carboximetoxifenil)-2-(4-sulfofenil)-2H-tetrazolio)/metosulfato de fenazina (MTS/PMS) y sulforodamina B, revelaron que sólo la fracción soluble en agua presenta efecto citotóxico. Adicionalmente, se realizó un estudio fitoquímico obteniéndose información de la presencia de algunas familias de metabolitos secundarios, tales como flavonoides, saponinas, taninos, polifenoles, antraquinonas, triterpenos y esteroles. Se puede inferir que la corteza del tallo de la planta V. cayennensis, es una fuente promisoria de metabolitos secundarios bioactivos. Palabras Clave: Actividad anti-inflamatoria; citotoxicidad; Escherichia coli; metabolitos secundarios; Shigella sp; Vismia cayennensis. AbstractA chemical and biological preliminary study of the species Vismia cayennensis, collected in the Amazonas state, Venezuela. The antibacterial test of plant bark extract showed significant inhibition in Escherichia coli, Shigella sp and Staphylococcus aureus. In addition, three of the five soluble fractions of different polarity solvents, specifically those of chloroform, acetone and water, maintained moderately active against Shigella sp strain. The hydroalcoholic extract of the plant and the fraction soluble in chloroform, exhibited a significant antiinflammatory effect. Cytotoxicity tests performed by the methods of (3-(4,5-dimetiltiazol-2-yl)-5-(3-carboximetoxifenil)-2-(4-sulfophenyl)-2H-tetrazolium)/phenazine methosulfate of (MTS/PMS) and sulforhodamine B, revealed that only has water soluble cytotoxic effect. Additionally, a study phytochemical obtained information on the presence of some families of secondary metabolites such as flavonoids, saponins, tannins, polyphenols, anthraquinones, triterpenes and sterols. It can be inferred that the stem bark of the plant V. cayennensis, is a promising source of bioactive secondary metabolites.

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“…Another compound used to treat sporotrichosis caused by S. schenckii ATCC 16345 was the ethanolic extract of Vismia guianensis , a tropical plant. The combination of the extracts plus ITC reduced the fungal load in the spleen of male Balb/c mice [ 80 ].…”

Section: Treatmentmentioning

confidence: 99%

Immunity and Treatment of Sporotrichosis

Carnero

Pérez

Hernández

et al. 2018

JoF

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Species of the Sporothrix complex are the etiological agents of sporotrichosis, an important subcutaneous mycosis with several clinical forms and an increasing incidence around the world that affects humans and other mammals. The immunological mechanisms involved in the prevention and control of this mycosis are not entirely understood. Many reports have suggested that cell-mediated immunity has an essential role in the development of the disease, being the primary response controlling it, while only recent data supports that the humoral response is essential for the appropriate control. This mycosis is a challenge for diagnosis since the culture and isolation of the organism are time-consuming and complicated; reasons that have led to the study of fungus antigenic molecules capable of generating a detectable humoral response. The treatment for this disease includes the use of several antifungal drugs like itraconazole, amphotericin B, caspofungin, fluconazole, and the combination between them among others such as the extract of Vismia guianensis.

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Anti-inflammatory activity of Vismia guianensis (Aubl.) Pers. extracts and antifungal activity against Sporothrix schenckii

Abstract: The results showed that both VGL and VGB have a significant antifungal against S. schenckii and an anti-inflammatory activity. These results can support the use of these extracts for alternative treatment of sporotrichosis.

Cited by 22 publications

References 48 publications

miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis

miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis

Estudio fitoquímico y biológico preliminar de la corteza (tallo) de Vismia cayennensis proveniente del estado Amazonas, Venezuela.// Phytochemical and biological preliminary study of Vismia cayennensis bark (steam) from Amazonas state, Venezuela

Immunity and Treatment of Sporotrichosis

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