Anti-Inflammatory and anti-arthritic activity of ethanolic extract of stem bark of saraca asoca

Abstract

The intension of the present study was to evaluate the ethanolic extract of stem bark of saraco asoca was assessed for its anti-inflammatory and antiarthritic activity by in vitro methods. The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, saponins, tannins, flavonoids, terpenoids, glycosides, xanthoproteins, and phenols. Results showed that the extract exhibited strong inhibitory activity. The total flavonoid and tannins content was found to be 0.015 and 0.03 mg in the stem bark of saraco asoca. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay, lipooxygenase inhibition, and membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of anti-inflammatory activity. Linear regression analysis was used to calculate IC50 value. Maximum inhibition of heat-induced protein denaturation of 72% was observed at 100 μg/ml, IC50 34.8+1.34 μg/ml). Hypo tonicity activity was also significantly inhibited (IC50 = 41.6+1.70 μg/ml). antilipooxygenase activity attributed minor protection by the bark extract with an (IC50 of 37.6+0.76 μg/ml). Antiarthritic activity performed by protein denaturation and HRBC membrane assay Antimicrobial analysis of the extracts was studied against common pathogens. The zone of inhibition was performed to determine the antimicrobial activity at different concentrations of plant extracts (60%,70%, 80%). It was observed that E. coli was inhibited at all concentrations, followed by Enterococcus and Pseudomonas.