Anti-inflammatory and Anti-nociceptive Actions of Systemically or Locally Treated Adipose-Derived Mesenchymal Stem Cells in Experimental Inflammatory Model

Mert, Tufan; Kurt, Akif Hakan; Arslan, Mahmut; Çelik, Ahmet; Tugtag, Berin; Akkurt, Aysenur

doi:10.1007/s10753-014-0101-1

Cited by 28 publications

(24 citation statements)

References 33 publications

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“…Nonetheless, the long-term use of NSAIDs is not recommended for a variety of patients, warranting the development of valid therapeutic alternatives, such as MSCs recently used for their anti-inflammatory and antinociceptive activity in an experimental inflammatory model (44). In the present study, neonatal MSCs were evaluated as an alternative to NSAIDs in the management of the pain and walk recovery of dogs operated using TPLO for CrCL rupture.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of a Single Intra-articular Injection of Allogeneic Neonatal Mesenchymal Stromal Cells Compared to Oral Non-Steroidal Anti-inflammatory Treatment on the Postoperative Musculoskeletal Status and Gait of Dogs over a 6-Month Period after Tibial Plateau Leveling Osteotomy: A Pilot Study

Taroni

Cabon

Febre³

et al. 2017

Front. Vet. Sci.

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ObjectiveCompare the clinical and pressure walkway gait evolution of dogs after a tibial plateau leveling osteotomy (TPLO) for a cranial cruciate ligament rupture (CrCLR) and treatment with either a 1-month course of non-steroidal anti-inflammatory drugs (NSAIDs) or a single postoperative intra-articular (IA) injection of allogeneic neonatal mesenchymal stromal cells (MSCs).Study designProspective, double-blinded, randomized, controlled, monocentric clinical study.AnimalsSixteen client-owned dogs.Materials and methodsDogs with unilateral CrCLR confirmed by arthroscopy were included. Allogeneic neonatal canine MSCs were obtained from fetal adnexa retrieved after C-section performed on healthy pregnant bitches. The dogs were randomly allocated to either the “MSCs group,” receiving an IA injection of MSCs after TPLO, followed by placebo for 1 month, or the “NSAIDs group,” receiving IA equivalent volume of MSCs vehicle after TPLO, followed by oral NSAID for 1 month. One of the three blinded evaluators assessed the dogs in each group before and after surgery (1, 3, and 6 months). Clinical score and gait and bone healing process were assessed. The data were statistically compared between the two groups for pre- and postoperative evaluations.ResultsFourteen dogs (nine in the MSCs group, five in the NSAIDs group) completed the present study. No significant difference was observed between the groups preoperatively. No local or systemic adverse effect was observed after MSCs injection at any time point considered. At 1 month after surgery, bone healing scores were significantly higher in the MSCs group. At 1, 3, and 6 months after surgery, no significant difference was observed between the two groups for clinical scores and gait evaluation.ConclusionA single IA injection of allogeneic neonatal MSCs could be a safe and valuable postoperative alternative to NSAIDs for dogs requiring TPLO surgery, particularly for dogs intolerant to this class of drugs.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Effect of a Single Intra-articular Injection of Allogeneic Neonatal Mesenchymal Stromal Cells Compared to Oral Non-Steroidal Anti-inflammatory Treatment on the Postoperative Musculoskeletal Status and Gait of Dogs over a 6-Month Period after Tibial Plateau Leveling Osteotomy: A Pilot Study

Taroni

Cabon

Febre³

et al. 2017

Front. Vet. Sci.

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“…Cheng et al in 2015 have demonstrated that Mesenchymal stem cells could attenuated inflammatory cytokines such as TNF- α , IL-17, IL-23, etc and promote the expression of TGF- β which is result in modulating the inflammation ( 18 ). Also related to anti-inflammatory character of MSCs, Mert et al in 2015 have investigated that the locally transplanted MSCs could suppress the level of IL-6 and (IL)-1 β and enhance IL-10 this effects of MSCs on these cytokines results in decreasing the inflammation ( 19 ). In addition above studies, Gu et al in 2014 showed that the expression levels of TNF- α , IL-1 β mRNA and P-I κ B- α , P-IKK β , p53 protein were significantly decreased and I κ B- α , Bcl-2 protein expression levels were significantly increased after brain stroke and mesenchymal stem cell transplantation ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

Combination Cell Therapy with Mesenchymal Stem Cells and Neural Stem Cells for Brain Stroke in Rats

Hosseini

Farahmandnia

Razi

et al. 2015

IJSC

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ObjectivesBrain stroke is the second most important events that lead to disability and morbidity these days. Although, stroke is important, there is no treatment for curing this problem. Nowadays, cell therapy has opened a new window for treating central nervous system disease. In some previous studies the Mesenchymal stem cells and neural stem cells. In this study, we have designed an experiment to assess the combination cell therapy (Mesenchymal and Neural stem cells) effects on brain stroke.Method and MaterialsThe Mesenchymal stem cells were isolated from adult rat bone marrow and the neural stem cells were isolated from ganglion eminence of rat embryo 14 days. The Mesenchymal stem cells were injected 1 day after middle cerebral artery occlusion (MCAO) and the neural stem cells transplanted 7 day after MCAO. After 28 days, the neurological outcomes and brain lesion volumes were evaluated. Also, the activity of Caspase 3 was assessed in different groups.ResultThe group which received combination cell therapy had better neurological examination and less brain lesion. Also the combination cell therapy group had the least Caspase 3 activity among the groups.ConclusionsThe combination cell therapy is more effective than Mesenchymal stem cell therapy and neural stem cell therapy separately in treating the brain stroke in rats.

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“…PMF exposure system and application procedures to rats were performed as previously described [Mert et al, , ]. Briefly, PMF exposure system was generated in a pair of Helmholtz coils 60 cm in diameter (placed 30 cm apart), with 100 turns of 18 gauge copper wire in a Faraday cage.…”

Section: Methodsmentioning

confidence: 99%

“…In this sense, cell‐based therapy can also be a capable approach for chronic neuropathic pain. Adipose tissue has been recently preferred as a suitable source of MSCs because it can be harvested easily from subcutaneous fat tissue [Mert et al, ]. Adipose tissue‐derived mesenchymal stem cells (ADMSC) have similar differentiation potentials and immunosuppressive capabilities to MSC derived from bone marrow [Strem et al, ].…”

Section: Introductionmentioning

confidence: 99%

Pulsed magnetic field enhances therapeutic efficiency of mesenchymal stem cells in chronic neuropathic pain model

Mert

Kurt

Altun

et al. 2017

Bioelectromagnetics

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Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therapy and magneto-cell therapy (combination of ADMSC and PMF) in chronic constriction nerve injury model (CCI). The actions of individual ADMSC (route dependent [systemic or local], time-dependent [a day or a week after surgery]), or PMF and their combination (magneto-cell) therapies on hyperalgesia and allodynia were investigated by using thermal plantar test and a dynamic plantar aesthesiometer, respectively. In addition, various cytokine levels (IL-1β, IL-6, and IL-10) of rat sciatic nerve after CCI were analyzed. Following the CCI, both latency and threshold significantly decreased. ADMSC or PMF significantly increased latencies and thresholds. The combination of ADMSC with PMF even more significantly increased latency and threshold when compared with ADMSC alone. However, ADMSC-induced decrease in pro-inflammatory or increase in anti-inflammatory cytokines levels were partially prevented by PMF treatments. Present findings may suggest that both cell-based and magnetic therapies can effectively attenuate chronic neuropathic pain symptoms. Combined magneto-cell therapy may also efficiently reverse neuropathic signs. Bioelectromagnetics. 38:255-264, 2017. © 2017 Wiley Periodicals, Inc.

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Anti-inflammatory and Anti-nociceptive Actions of Systemically or Locally Treated Adipose-Derived Mesenchymal Stem Cells in Experimental Inflammatory Model

Cited by 28 publications

References 33 publications

Combination Cell Therapy with Mesenchymal Stem Cells and Neural Stem Cells for Brain Stroke in Rats

Pulsed magnetic field enhances therapeutic efficiency of mesenchymal stem cells in chronic neuropathic pain model

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