Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice

Zarzecki, Micheli Stéfani; Bortolotto, Vandreza Cardoso; Poetini, Márcia Rósula; Araújo, Stífani Machado; Paula, Mariane Trindade de; Roman, Silvane Souza; Spiazzi, Cristiano Chiapinotto; Cibin, Francielli Weber Santos; Rodrigues, Oscar E. D.; Jessé, Cristiano Ricardo; Prigol, Marina

doi:10.1002/jcb.25670

Cited by 9 publications

(4 citation statements)

References 47 publications

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“…74 Oxidative stress and its consequent lipid peroxidation are known to damage cellular macromolecules such as DNA, lipids, and proteins, which could exacerbate free radical chain reactions and activate the release of pro-inflammatory mediators, disrupt the integrity of the intestinal mucosal barrier, and activate inflammatory mediators. 75 MPO is an enzyme abundantly found in neutrophils which has been used as a marker of cell-specific infiltration and the severity of inflammation. 76 As a marker for free radicals-induced lipid peroxidation, MDA is widely used as a biochemical marker of oxidative stress.…”

Section: Figurementioning

confidence: 99%

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Dou¹,

Zhou²,

Wang³

et al. 2018

IJN

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BackgroundBruceine D (BD) is a major bioactive component isolated from the traditional Chinese medicinal plant Brucea javanica which has been widely utilized to treat dysentery (also known as ulcerative colitis [UC]).MethodsTo improve the water solubility and absolute bioavailability of BD, we developed a self-nanoemulsifying drug delivery system (SNEDDS) composing of MCT (oil), Solutol HS-15 (surfactant), propylene glycol (co-surfactant) and BD. The physicochemical properties and pharmacokinetics of BD-SNEDDS were characterized, and its anti-UC activity and potential mechanism were evaluated in TNBS-induced UC rat model.ResultsThe prepared nanoemulsion has multiple beneficial aspects including small mean droplet size, low polydispersity index (PDI), high zeta potential (ZP) and excellent stability. Transmission electron microscopy showed that nanoemulsion droplets contained uniform shape and size of globules. Pharmacokinetic studies demonstrated that BD-SNEDDS exhibited enhanced pharmacokinetic parameters as compared with BD-suspension. Moreover, BD-SNEDDS significantly restored the colon length and body weight, reduced disease activity index (DAI) and colon pathology, decreased histological scores, diminished oxidative stress, and suppressed TLR4, MyD88, TRAF6, NF-κB p65 protein expressions in TNBS-induced UC rat model.ConclusionThese results demonstrated that BD-SNEDDS exhibited highly improved oral bioavailability and advanced anti-UC efficacy. In conclusion, our current results provided a foundation for further research of BD-SNEDDS as a potential complementary therapeutic agent for UC treatment.

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Section: Figurementioning

confidence: 99%

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Dou¹,

Zhou²,

Wang³

et al. 2018

IJN

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“…35 In addition, the activation of these cells causes oxidative stress by increasing RS levels. 36 Research has suggested that antioxidant systems become impaired in RA, 33,37−40 and this is particularly important as our data revealed lower NPSH levels and SOD activity in the cerebral cortex, hippocampus, and paw tissues of CFAexposed. The results of the NPSH content analyzed after applying CFA and/or Se-DMC or meloxicam treatments are listed in Table 1.…”

Section: Effects Of Se-dmc On Paw Histologicalmentioning

confidence: 52%

“…There is a close link between oxidative stress and inflammation, and NF-κB plays a vital role in this process by balancing the synthesis and release of proinflammatory substances . In addition, the activation of these cells causes oxidative stress by increasing RS levels …”

Section: Resultsmentioning

confidence: 99%

Se-[(2,2-Dimethyl-1,3-dioxolan-4-yl)methyl] 4-Chlorobenzoselenolate Attenuates Inflammatory Response, Nociception, and Affective Disorders Related to Rheumatoid Arthritis in Mice

Wilhelm

Soares

Reis

et al. 2021

ACS Chem. Neurosci.

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Despite major advances, not all patients achieve rheumatoid arthritis (RA) remission, thus highlighting a pressing need for new therapeutic treatments. Given this scenario, this study sought to evaluate Se-[(2,2-dimethyl-1,3-dioxolan-4-yl)methyl] 4chlorobenzoselenolate (Se-DMC) potential on a complete Freund's adjuvant (CFA)-induced unilateral arthritis model. The effects of Se-DMC (5 mg/kg; oral dose) and meloxicam (5 mg/kg; oral dose), both administered to animals daily for 14 days, on paw edema, mechanical sensitivity, neurobehavioral deficits (anxiogenic-and depressive-like behaviors), Na + /K + -ATPase activity, oxidative stress, and inflammation were evaluated in male Swiss mice exposed to CFA (intraplantar injection of 0.1 mL; 10 mg/ mL). Se-DMC reduced the paw withdrawal threshold and CFA-induced paw edema. Histopathological results revealed the antiedematogenic potential of the compound, which was evidenced by lower quantities of dilated lymphatic vessels compared with the CFA group. Se-DMC reduced mRNA relative expression levels of tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in the hippocampus and paw of CFA mice. The CFA-induced anxiogenic-and depressive-like behaviors were reversed by Se-DMC to the control levels in the elevated plus-maze and tail suspension tests. Se-DMC reduced the paw reactive species levels and restored the superoxide dismutase (hippocampus and paw) and Na + /K + -ATPase (hippocampus) activities previously increased by CFA. Moreover, CFA administration inhibited serum creatinine kinase activity, albeit the Se-DMC effects did not appear to involve the modulation of this enzyme and were equal to or greater than meloxicam. Se-DMC attenuates CFA-induced inflammatory response, nociception, and neurobehavioral deficits in mice.

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“…Although some evaluated parameters did not differ significantly among the different treatments with L. lactis, it was noteworthy that compared with the C+ group, the NFXi group presented a decrease in the severity of inflammation as indicated by reduced body weight loss and decreases in macroscopic and histological scores. The evaluation of these parameters was important because colitis caused by intrarectal administration of TNBS causes abrupt weight loss, rectal prolapse and transmural inflammation (Kiesler et al 2015;Zarzecki et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Invasive Lactococcus lactis producing mycobacterial Hsp65 ameliorates intestinal inflammation in acute TNBS‐induced colitis in mice by increasing the levels of the cytokine IL‐10 and secretory IgA

Cunha

Preisser

Santana

et al. 2020

J. Appl. Microbiol.

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Aims: To investigate the anti-inflammatory activity of an invasive and Hp65producing strain Lactococcus lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) in acute 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in mice as an innovative therapeutic strategy against Crohn's disease (CD). Methods and Results: The pXYCYT:Hsp65 plasmid was transformed into the L. lactis NCDO2118 FnBPA+ strain, resulting in the L. lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) strain. Then, the functionality of the strain was evaluated in vitro for Hsp65 production by Western blotting and for invasion into Caco-2 cells. The results demonstrated that the strain was able to produce Hsp65 and efficiently invade eukaryotic cells. Subsequently, in vivo, the anti-inflammatory capacity of the recombinant strain was evaluated in colitis induced with TNBS in BALB/c mice. Oral administration of the recombinant strain was able to attenuated the severity of colitis by mainly reducing IL-12 and IL-17 levels and increasing IL-10 and secretory immunoglobulin A levels. Conclusions: The L. lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) strain contributed to a reduction in inflammatory damage in experimental CD. Significance and Impact of the Study: This study, which used L. lactis for the production and delivery of Hsp65, has scientific relevance because it shows the efficacy of this new strategy based on therapeutic protein delivery into mammalian enterocytes.

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Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice

Cited by 9 publications

References 47 publications

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Se-[(2,2-Dimethyl-1,3-dioxolan-4-yl)methyl] 4-Chlorobenzoselenolate Attenuates Inflammatory Response, Nociception, and Affective Disorders Related to Rheumatoid Arthritis in Mice

Invasive Lactococcus lactis producing mycobacterial Hsp65 ameliorates intestinal inflammation in acute TNBS‐induced colitis in mice by increasing the levels of the cytokine IL‐10 and secretory IgA

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