Anti-inflammatory and anti-oxidative effects of herbal preparation EM 1201 in adjuvant arthritic rats

Akramas, Laimis; Leonavičienė, Laima; Vasiliauskas, Audrius; Bradūnaitė, Rūta; Vaitkienė, Dalia; Zabulytė, D.; Normantienė, Teresa; Lukošius, Audronis; Jonauskiene, İrena

doi:10.1016/j.medici.2015.11.002

Cited by 11 publications

(11 citation statements)

References 39 publications

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“…This effect of the herbal formulations was comparable to that obtained from the orthodox drug used for this study. This finding agrees with the work of Akramas et al [39] who reported similar findings using EM1201, a polyherbal formulation and diclofenac, an orthodox drug. Natural substances exert their medicinal effects in ways similar to allopathic medicines; they inhibit the pathways of inflammation [40].…”

Section: Discussionsupporting

confidence: 93%

Amelioration of Extra-articular Effects Associated with Rheumatoid Arthritis Using Anti-arthritic Herbal Formulations

Elechi-Amadi

Briggs

Opusunju

et al. 2020

JOCAMR

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Aim: This study investigated the ability of some herbal formulations to ameliorate extra-articular effects of some herbal formulations used in the management of rheumatoid arthritis in Nigeria. Methodology: Forty-nine (49) female albino Wistar rats were used for this study. They were divided into seven groups: A, B, C, D, E, F and G of seven rats each, with Group A serving as negative control while Group B was a positive control. Groups B, C, D, E, F and G were induced with rheumatoid arthritis by injecting 0.1 ml of Complete Freund's Adjuvant into the right hind paw of each rat. The rats were treated with the standard drug and herbal formulations respectively for 28 days as follows: Group C (treated with a standard drug, Celebrex), Group D (treated with the herbal drug, Jointeez), Group E (treated with a herbal drug, Arthropower), Group F (treated with combination therapy of Jointeez and Celebrex) and Group G (treated with combination therapy of Arthropower and Celebrex). At the end of the 28-day treatment period, the rats were anaesthetized with chloroform and sacrificed through puncture of the jugular vein. Five millilitres (5 ml) of blood samples were put into plain bottles for the analysis of biochemical parameters and 3 ml into K3EDTA bottles for haematological analysis. The lipid parameters were analysed using Mindray autoanalyzer while haematological parameters were determined using Sysmex haematology auto analyzer. Results: Total cholesterol (p<0.001), HDL (p=0.005) and LDL (p=0.004) were significantly reduced in the treated rats compared to the positive control group. Conversely, Packed Cell Volume (p<0.001) and Haemoglobin levels (p<0.001) were significantly reduced in the positive control rats compared to the treated rats. However, Total WBC count was significantly higher in the positive control rats than in the treated rats (p=0.001). The combination therapies used in this study did not offer a significantly different therapeutic advantage over the monotherapies used. The herbal formulations gave therapeutic effects on the extra-articular effects similar to that obtained from the orthodox drug used in this study. Conclusion: The herbal formulations can be used as alternative regimens for rheumatoid arthritis. It is recommended that herbal formulations be considered for integration into our healthcare system for the management of rheumatoid arthritis.

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Section: Discussionsupporting

confidence: 93%

Amelioration of Extra-articular Effects Associated with Rheumatoid Arthritis Using Anti-arthritic Herbal Formulations

Elechi-Amadi

Briggs

Opusunju

et al. 2020

JOCAMR

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“…This was accompanied by an increase in the circulation of inflammatory cells, which was confirmed by an increase in spleen weight and erythrocyte sedimentation rate. It has also been shown that cytokines released by inflamed synovial tissue can reach systemic circulation and act on other organs as proven in this study and by others [7, 21, 39, 40]. The systemic manifestations of arthritis were confirmed by a dramatic drop in the rats’ body weight and by impairing the liver.…”

Section: Discussionsupporting

confidence: 82%

Therapeutic benefits of Indole-3-Carbinol in adjuvant-induced arthritis and its protective effect against methotrexate induced-hepatic toxicity

Hasan

Ismail

El-Orfali

et al. 2018

BMC Complement Altern Med

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BackgroundRheumatoid arthritis (RA) being an incapacitating disease requires early effective intervention. Considering Methotrexate (MTX)- the first line of treatment for RA- intoxicates the liver; therefore, alternative therapies with similar efficacy yet lower cytotoxicity are desired. Indole-3-Carbinol (I3C) which is found in cruciferous vegetables was examined for its possible therapeutic potentials in comparison with MTX by investigating its anti-inflammatory, anti-arthritic, anti-oxidant, and hepatoprotective potentials in adjuvant-induced arthritis (AIA) rat model.MethodsArthritis was induced in Sprague Dawley rats by injection of Complete Freund’s Adjuvant (CFA). Arthritic rats were treated with I3C and/or MTX. To examine the anti-inflammatory and anti-arthritic effect, the following parameters were assessed: body weight, macroscopic scoring of the hind paw, the level of the pivotal cytokines (TNF-α, IL-6) heavily involved in the pathogenesis, spleen index, and erythrocyte sedimentation rate. At a histological level, the tibiotarsal joint was stained with several specific stains. To assess the hepatoprotective and anti-oxidant effects, several oxidative stress parameters were monitored, and the liver histology was examined.ResultsBoth I3C and MTX attenuated the inflammation that was aggravated by arthritis by downregulating the inflammatory markers and mediators and alleviating the histopathological changes affecting the tibiotarsal joint. I3C attenuated the liver impairment that was initiated by arthritis and MTX treatment. It did so by downregulating the pro-oxidants and up-regulating the anti-oxidant defenses and by reducing the pathological changes affecting the liver.ConclusionOur results suggest that I3C is as potent as MTX as an anti-inflammatory and anti-arthritic agent. In addition, I3C does so while protecting the liver from damage as opposed to MTX.

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“…MDA was increased during RA, suggesting that MDA was deemed a critical injury mechanism. A decrease in GSH could impair the clearance of H2O2 and support OH formation, enhancing the free radical load that leads to homeostasis disturbance . In addition, many trials have shown that there is a lot of oxidative stress in RA patients and this oxidative stress can lead to overproduction of cytokines and harm to the joint tissue .…”

Section: Discussionmentioning

confidence: 99%

“…A decrease in GSH could impair the clearance of H2O2 and support OH formation, enhancing the free radical load that leads to homeostasis disturbance. [30][31][32] In addition, many trials have shown that there is a lot of oxidative stress in RA patients and this oxidative stress can lead to overproduction of cytokines and harm to the joint tissue. 33,34 CFA rats have been shown to have reduced antioxidant enzyme activity in the plasma, such as SOD, CAT, and GSH, and some trials have shown that these antioxidant enzymes have RA impacts.…”

Section: Discussionmentioning

confidence: 99%

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Huang¹,

Guo

Chitti³

et al. 2019

BioFactors

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Rheumatoid arthritis (RA) is a chronic and accelerated autoimmune illness with proliferative and damaging synovitis, resulting in joint death and cartilage and bone erosion. This study focused on the potential therapeutic effect of wogonin on complete Freund's adjuvant (CFA) induced RA in rats and the underlying mechanisms. Arthritis was experimentally caused in rats by subcutaneously injecting 0.1 mL of CFA into the subplantar area of the left hind paw under moderate anesthesia on day zero. The regular oral doses of indomethacin/wogonin began on day zero and proceeded after injection to day 35. Wogonin reduced arthritic score considerably, enhanced body weight, and reduced paw thickness. Wogonin also boosted red blood cell considerably along with hemoglobin and reduced white blood cell count and erythrocyte sedimentation rate. Wogonin substantially improved an altered level of oxidative stress markers, antioxidant proteins, and inflammatory cytokines in a dose‐dependent way. Wogonin inhibited p38 phosphorylation triggered by CFA and p65 nuclear translocation.

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Anti-inflammatory and anti-oxidative effects of herbal preparation EM 1201 in adjuvant arthritic rats

Cited by 11 publications

References 39 publications

Amelioration of Extra-articular Effects Associated with Rheumatoid Arthritis Using Anti-arthritic Herbal Formulations

Amelioration of Extra-articular Effects Associated with Rheumatoid Arthritis Using Anti-arthritic Herbal Formulations

Therapeutic benefits of Indole-3-Carbinol in adjuvant-induced arthritis and its protective effect against methotrexate induced-hepatic toxicity

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

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