Anti-Inflammatory and Antinociceptive Effects of Salbutamol on Acute and Chronic Models of Inflammation in Rats: Involvement of an Antioxidant Mechanism

Abstract

<jats:p>The possible role of<jats:italic>β</jats:italic>-2 adrenergic receptors in modulation of inflammatory and nociceptive conditions suggests that the<jats:italic>β</jats:italic>-2 adrenergic receptor agonist, salbutamol, may have beneficial anti-inflammatory and analgesic effects. Therefore, in this study, we induced inflammatory and nociceptive responses with carrageenan-induced paw edema or cotton-pellet-induced granuloma models, both of which result in oxidative stress. We hypothesized that salbutamol would prevent inflammatory and nociceptive responses by stimulating<jats:italic>β</jats:italic>-2 adrenergic receptors and the prevention of generation of ROS during the acute inflammation process in rats. Both doses of salbutamol used in the study (1 and 2 mg/kg) effectively blocked the acute inflammation and inflammatory nociception induced by carrageenan. In the cotton-pellet-induced granuloma test, both doses of salbutamol also significantly decreased the weight of granuloma tissue on the cotton pellets when compared to the control. Anti-inflammatory and analgesic effects of salbutamol were found to be comparable with those of indomethacin. Salbutamol decreased myeloperoxidase (MPO) activity and lipid peroxidation (LPO) level and increased the activity of superoxide dismutase (SOD) and level of glutathione (GSH) during the acute phase of inflammation. In conclusion, salbutamol can decrease acute and chronic inflammation, possibly through the stimulation of<jats:italic>β</jats:italic>-2 adrenergic receptors. This anti-inflammatory effect may be of significance in asthma treatment, where inflammation also takes part in the etiopathology. This study reveals that salbutamol has significant antioxidative effects, which at least partially explain its anti-inflammatory capabilities. These findings presented here may also shed light on the roles of<jats:italic>β</jats:italic>-2 adrenergic receptors in inflammatory and hyperalgesic conditions.</jats:p>

Inflammation and peripheral 5-HT7 receptors: The role of 5-HT7 receptors in carrageenan induced inflammation in rats

“…SOD and GSH are the main components involved in the antioxidative system and limit the cytotoxic effects of toxic free radicals (Fink, 2002). Many inflammation studies have demonstrated reduced antioxidant levels in paw tissues (Halici et al, 2007;Uzkeser et al, 2012). In the present study, AS-19 increased antioxidant enzyme activity in paws, which had been significantly decreased by carrageenan induced inflammation.…”

Microemulsion-Based Topical Hydrogels of Tenoxicam for Treatment of Arthritis

“…The granuloma is characterized by the formation of a fibrous vascularized capsule containing fibroblast and mononuclear infiltrate cells. Decreased granuloma tissue formation, prevention of occurrence of the collagen fibres and suppression of mucopolysaccharides are indicators of the anti-proliferative effect of NSAIDs (41). Daily topical application of the microemulsion formulations TNX 03 and TNX 04 significantly (p<0.001) inhibited granulomatous tissue formation, in comparison to TNX 01, TNX 02 and control (Fig.…”

Riparin A, a compound from Aniba riparia, attenuate the inflammatory response by modulation of neutrophil migration

“…This study was aimed at evaluating the anti-inflammatory effect 37 of riparin A against acute inflammation. The results of our evaluations in various experimental models 38 indicated that riparin A reduced paw edema induced by carrageenan, compound 48/80, histamine, and 39 serotonin. Furthermore, it decreased leukocyte and neutrophil counts, myeloperoxidase activity, thiobar- 40 bituric acid reactive substance (TBARS) levels, and cytokine (tumor necrosis factor-a and interleukin-1b) 41 levels increased by carrageenan-induced peritonitis, and reversed glutathione levels.…”