Anti-inflammatory and antioxidant potential of Guaianolide isolated from Cyathocline purpurea : Role of COX-2 inhibition

Tambewagh, Urmila U.; Kandhare, Amit D.; Honmore, Varsha S.; Kadam, Parag P.; Khedkar, Vijay M.; Bodhankar, Subhash L.; Rojatkar, Supada R.

doi:10.1016/j.intimp.2017.09.001

Cited by 36 publications

(24 citation statements)

References 64 publications

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“…It has demonstrated that NO is an important mediator in ANTU‐induced PAH . NO is a stable free‐radical gaseous intracellular messenger existing in various isoforms, and eNOs is one of the isoforms responsible for generation of vascular endothelium NO . eNOs regulates cellular proliferation, platelet aggregation, vascular tone, and leukocyte adhesion .…”

Section: Discussionmentioning

confidence: 99%

Chrysin ameliorates ANTU‐induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs

Wang

Zhang

2019

J Biochem & Molecular Tox

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Alpha-naphthylthiourea (ANTU), a rodenticide induces lung toxicity. Chrysin a flavonoid possesses antioxidant, anti-inflammatory, and antihypertensive potential.The aim of this study was to evaluate the efficacy of chrysin against ANTU-induced pulmonary edema (PE) and pulmonary arterial hypertension (PAH) in laboratory rats.Sprague-Dawley rats were used to induce PE (ANTU, 10 mg/kg, ip) and PAH (ANTU, 5 mg/kg, ip, 4 weeks). Animals were treated with chrysin (10, 20, and 40 mg/kg) and various biochemical, molecular, and histological parameters were evaluated. Acute administration of ANTU induces PE revealed by significant (P < 0.05) increase in relative lung weight, pleural effusion volume, lung edema, bronchoalveolar lavage fluid cell counts, total protein, 5-hydroxytryptamine (5-HT), lactate dehydrogenase (LDH), and γ-glutamyl transferase (GGT), whereas pretreatment with chrysin (20 and 40 mg/kg, ip) significantly (P < 0.05) attenuated these ANTU-induced biochemical and histological alterations. Repeated administration of ANTU caused induction of PAH evaluated by significant (P < 0.05) alterations in electrocardiographic, hemodynamic changes, and left ventricular function, whereas chrysin (20 and 40 mg/kg, p.o.) treatment significantly (P < 0.05) attenuated these alterations. ANTU-induced hematological and serum biochemical (aspartate transaminase, alanine transaminase, LDH, and creatinine kinase MB) alterations were significantly (P < 0.05) inhibited by chrysin. It also significantly (P < 0.05) decreased elevated levels of oxido-nitrosative stress in the right ventricle (RV) and lung. Chrysin significantly (P < 0.05) attenuated downregulated endothelial nitric oxide synthase and upregulated vascular endothelial growth factor messenger RNA and protein expressions both in the RV and pulmonary artery. Chrysin inhibited ANTU-induced PE and PAH via modulation of inflammatory responses (5-HT, LDH, and GGT), oxido-nitrosative stress, and VEGF and eNOs levels.K E Y W O R D S alpha-naphthylthiourea, chrysin, eNOs, pulmonary arterial hypertension, pulmonary edema, vascular endothelial growth factor J Biochem Mol Toxicol. 2019;33:e22332.wileyonlinelibrary.com/journal/jbt

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Section: Discussionmentioning

confidence: 99%

Chrysin ameliorates ANTU‐induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs

Wang

Zhang

2019

J Biochem & Molecular Tox

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“…The close involvement of reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide (H2O2), and hydroxyl radical (OH) resulted in many dreaded diseases like cancer, Alzheimer's disease, aging, inflammation, rheumatoid arthritis, and atherosclerosis [20,21]. Now a day various studies havebeen reported on the crucial role of antioxidant and radical-scavenging mechanism in various antiinflammatory, anti-arthritic, anti-diabetic, anti-convulsant, antiulcer, cardioprotective, hepatoprotective, neuroprotective, and wound healing potential of drugs [22][23][24]. The 2-diphenyl-1picrylhydrazyl (DPPH), H2O2 and hydroxyl radical scavenging assay are few of them.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antioxidant and in Vivo Anti-Inflammatory Activity of the Aerial Part of Blumea Eriantha Dc

Tambewagh

Rojatkar

2018

Int J Pharm Pharm Sci

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Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of methanol extract of aerial part of the Blumea eriantha DC belonging to family Asteraceae.Methods: The shade dried aerial part of B. eriantha (0.5 kg) was powdered and extracted with methanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of methanol extract of aerial part of Blumea eriantha DC was evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radicalscavenging and reducing power assays.Results: The results indicate that methanol extract of Blumea eriantha (BEME, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BEME showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100µg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the methanol extract of Blumea eriantha The methanol extract was found to be safe at the dose of 2000 mg/kg.Conclusion: The results of the experimental study confirmed that methanol extract of Blumea eriantha DC possesses significant anti-inflammatory and antioxidant activity.

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“…Before 1 h of injecting of carrageenan, the rats were treated with different doses of H. plantifolius, ibuprofen, and 10% DMSO [6]. The volume of paw edema was measured by dislocation of the water column in a plethysmometer [7] immediately after carrageenan injection at 0, 1, 2, 3, 4, and 5 h. The average paw volume was measured and compared with control and standard groups. Reduction in the paw volume in H. plantifolius pretreated groups compared with the control animals was considered as anti-inflammatory response ( Table 1).…”

Section: Carrageenan-induced Edema In Rat Hind Pawmentioning

confidence: 99%

Anti-Inflammatory Activity of Methanolic Extract of Hibiscus Plantifolius

Sowjanya¹,

Swati²,

Supriyanka³

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of the present study was to carry out anti-inflammatory activity of methanolic extract of Hibiscus plantifolius (MEHP) belonging to the family Malvaceae.Methods: The shade dried stem part of H. plantifolius (1 kg) was powdered and extracted with methanol using soxhletion. The extract was concentrated using rotary evaporator under reduced pressure at 40°C, till free from the solvents and thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level of 50, 150, and 300 mg/kg. Acute oral toxicity study was also studied.Results: The results indicate that MEHP, 300 mg/kg, exhibited significant inhibition (p<0.001) of increase in paw edema at 4th h.Conclusion: The results of the experimental study confirmed that MEHP possesses significant anti-inflammatory activity.

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Anti-inflammatory and antioxidant potential of Guaianolide isolated from Cyathocline purpurea : Role of COX-2 inhibition

Cited by 36 publications

References 64 publications

Chrysin ameliorates ANTU‐induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs

Chrysin ameliorates ANTU‐induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs

In Vitro Antioxidant and in Vivo Anti-Inflammatory Activity of the Aerial Part of Blumea Eriantha Dc

Anti-Inflammatory Activity of Methanolic Extract of Hibiscus Plantifolius

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