Anti-inflammatory and immune-modulatory properties of anemoside B4 isolated from Pulsatilla chinensis in vivo

Kang, Naixin; Shen, Wenhua; Zhang, Yong; Su, Zhetong; Yang, Shilin; Li, Yanli; Xu, Qiong

doi:10.1016/j.phymed.2019.152934

Cited by 59 publications

(55 citation statements)

References 28 publications

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“…Western bolt analysis was performed as previously described [27]. In brief, proteins were extracted in RIPA lysis buffer, separated by SDS-PAGE, and transferred into PVDF membranes.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…In this study, TNBS-induced severe acute colitis rat model was used to determine the pharmacological activity of anemoside B4. The doses of anemoside B4 were chosen on the basis of our preliminary tests of LPS-induced in ammation and acetic acid-induced analgesia [27]. Mesalazine was orally administrated to rats with the same dose as the positive control [31].…”

Section: Anemoside B4 Ameliorated Tnbs-induced Colitis In Ratsmentioning

confidence: 99%

“…Our previous study reported that anemoside B4, the most abundant triterpenoid saponin isolated from P. chinensis, decreases in ammation and oxidant injury induced by cisplatin. It ameliorates LPS-induced kidney and lung damage, inhibits LPS-induced NF-κB activation in vivo [26,27]. However, it is unclear whether anemoside B4 exhibits effective roles in treating IBD and it might be the molecular basis for the clinical effect of P. chinensis.…”

Section: Introductionmentioning

confidence: 99%

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Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Zhang¹,

Zha²,

Shen³

et al. 2020

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Background Despite the increased morbidity of ulcerative colitis (UC) in the developing countries, available treatments remain unsatisfactory. Therefore, it is urgent to discover more effective therapeutic strategies. Pulsatilla chinensis was widely used for the treatment of inflamed intestinal diseases including UC for thousands of years in China. However, it is unclear which compound in P. chinensis is responsible for the therapeutic effect. Our previous study reported that anemoside B4, the most abundant triterpenoid saponin isolated from P. chinensis, exerts anti-inflammatory and antioxidant effects. Methods Here, we used the 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model to evaluate the therapeutic effect of anemoside B4. Blood samples of colitis rat were collected for hematology analysis. The effects of anemoside B4 on inflammation-associated mediators were investigated by Enzyme-linked immunosorbent assay (ELISA) and hematoxylin and eosin staining (HE) staining. Cell proliferation or apoptosis was measured by immunofluorescence technique. The mechanisms of anemoside B4 was investigated using label-free quantitative proteomics. The level of proteins was quantified by western blotting. mRNA expression was quantified by quantitative real-time RT-PCR. Results The results showed that anemoside B4 ameliorated TNBS-induced colitis symptoms, including tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine production, apoptosis and slowed proliferation in the colon. Quantitative proteomic analyses discovered that 56 proteins were significantly altered by anemoside B4 in the TNBS-induced rats. These proteins were mainly clustered in tricarboxylic acid cycle (TCA) cycle and respiratory electron transport chain. Among the altered proteins, S100A9 is one of the most significantly downregulated proteins and associated with NF-κB and MAPK signaling pathways in the pathogenesis of UC. Further experiments revealed that anemoside B4 suppresses the expression of S100A9 and its downstream genes including TLR4, NF-κB, and p-JNK in colon. In vitro, S100A9 protein could active NF-κB signaling pathway in human intestinal epithelial Caco-2 cells. However, anemoside B4 could inhibit the NF-κB signaling pathway induced by S100A9 protein. Besides, it also inhibited active of NF-κB signaling pathway stimulated by LPS or IL-6. Conclusions Our results demonstrate that anemoside B4 prevents TNBS-induced colitis involved inhibiting the NF-κB signaling pathway through inactivating S100A9 suggesting that anemoside B4 is a promising therapeutic candidate for colitis.

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“…Western bolt analysis was performed as previously described [27]. In brief, proteins were extracted in RIPA lysis buffer, separated by SDS-PAGE, and transferred into PVDF membranes.…”

Section: Western Blot Analysismentioning

confidence: 99%

Section: Anemoside B4 Ameliorated Tnbs-induced Colitis In Ratsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Zhang¹,

Zha²,

Shen³

et al. 2020

Preprint

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“…Because of antibiotic resistance, an alternative treatment of mastitis with the lower side effects and lower risk of drug resistance is becoming increasingly desirable [7]. Pulsatilla saponin B4 (Anemoside B4, Chemical Abstracts Service number: 129741-57-7) is the main active ingredient of Pulsatilla Chinensis and it has a therapeutic role in the case of mastitis as an anti-in ammatory, anti-bacterial, and immune regulating agent [8]. Thus, our study focused on the treatment e ciency and the in uence of Pulsatilla saponin B4 on the serum in ammatory factors in the cows with mastitis.…”

Section: Introductionmentioning

confidence: 99%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Liu

Qian

et al. 2020

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Background To investigate the effect of Pulsatilla saponin B4, a purified extract form Pulsatilla Chinensis, on the treatment of clinical mastitis in dairy cows, 12 healthy cows were used as the control group (group A, no treatment), and 36 cows with mastitis were divided into 3 groups based on the quaque die (QD) intramuscular injection (IM) dose used in the as follows, group B (15 mL), group C (30 mL), group D (60 mL). Identified the microorganisms in milk during the experiment,samples in test groups were cultured, isolated, and verified by quantitative real-time polymerase chain reaction (qPCR). Somatic cell count (SCC) and serum inflammatory indices in cows were measured with a 1-day interval in group C and compared with group A. Results Results showed that bacterial detection rates were 100% (group B), 83.33% (group C) and 100% (group D). After the treatment bacterial detection rates dropped to 50.00% (group B), 50.00% (group C), and 41.67% (group D). The medicine was effective in all test groups and showed a dose-effect relationship. Serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4), leukotriene B4 (LTB4) also the SCC were significantly higher than healthy cows (P < 0.05) on the day (d) 1 in group C but had no significant difference with group A after treatment. Conclusion Treatment with Pulsatilla saponin B4 relieved mastitis, leading to relieve of bacterial infection, SCC and levels of serum inflammatory factors effectively in cows with mastitis after being administered for 4–6 days QD.

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“…Recent studies have shown that triterpenoid saponins are the main factors affecting the pharmacological activities in this herb. Anemoside B4, one of the main monomer components of Pulsatilla chinensis (Bunge) Regel, can inhibit the pathogenesis of acute kidney injury caused by cisplatin and improve renal function, which protective effects may be associated with its anti-in ammatory activities [24]{Pociecha, 2017 #366}.…”

Section: Introductionmentioning

confidence: 99%

Anemoside B4 protects against Klebsiella pneumoniae- and influenza virus FM1-induced pneumonia via the TLR4/Myd88 signaling pathway in mice

Yuan

Cui

et al. 2020

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Abstract Background: Pneumonia refers to the inflammation of the terminal airway, alveoli and pulmonary interstitium, which can be caused by pathogenic microorganisms, physical and chemical factors, immune damage, and drugs. Anemoside B4, the major ingredient of Pulsatilla chinensis (Bunge) Regel, exhibited anti-inflammatory activity. However, the therapeutic effect of anemoside B4 on pneumonia has not been unraveled. This study aims to investigate that anemoside B4 attenuates the inflammatory responses in Klebsiella pneumonia (KP)- and influenza virus FM1 (FM1)-induced pneumonia mice model.Methods: The network pharmacology and molecular docking assays were employed to predict the targets of anemoside B4’s treatment of pneumonia. Two models (bacterial KP-infected mice and virus FM1-infected mice) were employed in our study. BALB/c mice were divided into six groups: control, model group (KP- induced pneumonia or FM1-induced pneumonia), anemoside B4 (B4)-treated group (2.5, 5, 10 mg/kg), and positive drug group (Ribavirin or Ceftriaxone Sodium Injection). Blood samples were collected for hematology analysis. The effects of B4 on inflammation-associated mediators were investigated by Enzyme-linked immunosorbent assay (ELISA) and hematoxylin and eosin staining (HE) staining. Proteins expression was quantified by western blotting.Results: The network results indicated that many pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) participated in anemoside B4’s anti-inflammatory activity. The counts of neutrophil (NEU) and white blood cell (WBC), the level of myeloperoxidase (MPO), and the release of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 increased by KP or FM1 infection, which were reversed by anemoside B4. In addition, anemoside B4 significantly suppressed the FM1-induced expression of Toll-like receptor 4 (TLR4), myeloid differential protein-88 (MyD88), and myeloid differentiation protein-2 (MD-2), which were further validated by molecular docking data that anemoside B4 bound to bioactive sites of TLR4. Therefore, anemoside B4 exhibited a significant therapeutic effect on pneumonia via the TLR4/MyD88 pathway.Conclusion: Our findings demonstrated that anemoside B4 attenuates pneumonia via the TLR4/Myd88 signaling pathway, suggesting that anemoside B4 is a promising therapeutic candidate for bacterial-infected or viral-infected pneumonia.

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Anti-inflammatory and immune-modulatory properties of anemoside B4 isolated from Pulsatilla chinensis in vivo

Cited by 59 publications

References 28 publications

Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Anemoside B4 protects against Klebsiella pneumoniae- and influenza virus FM1-induced pneumonia via the TLR4/Myd88 signaling pathway in mice

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