Anti-inflammatory and immunomodulatory effects of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats

Zhu, Wenjing; Pang, Ming-qun; Dong, Liuyi; Huang, Xueying; Wang, Shuangmiao; Zhou, Lanlan

doi:10.1016/j.lfs.2012.08.013

Cited by 49 publications

(23 citation statements)

References 22 publications

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“…Currently, four methods have been documented in the literature outlining methods of establishing HN animal models, including use of a urate oxidase inhibitor [43–45], purine-rich diets [46], administration of a mixture of adenine and potassium oxonate [47–50] and disruption of the Uox gene leading to uricase deficiency in mice [51–53]. Among them, administration of a mixture of adenine and potassium oxonate is the most common and effective method to establish the model of hyperuricemic nephropathy [47–50].…”

Section: Discussionmentioning

confidence: 99%

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Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats

Liu

Shi

et al. 2017

Oncotarget

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The pathogenesis of hyperuricemia-induced chronic kidney disease is largely unknown. In this study, we investigated whether extracellular signal–regulated kinases1/2 (ERK1/2) would contribute to the development of hyperuricemic nephropathy (HN). In a rat model of HN induced by feeding mixture of adenine and potassium oxonate, increased ERK1/2 phosphorylation and severe glomerular sclerosis and renal interstitial fibrosis were evident, in parallel with diminished levels of renal function and increased urine microalbumin excretion. Administration of U0126, which is a selective inhibitor of the ERK1/2 pathway, improved renal function, decreased urine microalbumin and inhibited activation of renal interstitial fibroblasts as well as accumulation of extracellular proteins. U0126 also inhibited hyperuricemia-induced expression of multiple profibrogenic cytokines/chemokines and infiltration of macrophages in the kidney. Furthermore, U0126 treatment suppressed xanthine oxidase, which mediates uric acid production. It also reduced expression of the urate anion exchanger 1, which promotes reabsorption of uric acid, and preserved expression of organic anion transporters 1 and 3, which accelerate uric acid excretion in the kidney of hyperuricemic rats. Finally, U0126 inhibited phosphorylation of Smad3, a key mediator in transforming growth factor (TGF-β) signaling. In cultured renal interstitial fibroblasts, inhibition of ERK1/2 activation by siRNA suppressed uric acid-induced activation of renal interstitial fibroblasts. Collectively, pharmacologic targeting of ERK1/2 can alleviate HN by suppressing TGF-β signaling, reducing inflammation responses, and inhibiting the molecular processes associated with elevation of blood uric acid levels in the body. Thus, ERK1/2 inhibition may be a potential approach for the prevention and treatment of hyperuricemic nephropathy.

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Section: Discussionmentioning

confidence: 99%

“…Among them, administration of a mixture of adenine and potassium oxonate is the most common and effective method to establish the model of hyperuricemic nephropathy [47–50]. As a result, we choose this model.…”

Section: Discussionmentioning

confidence: 99%

Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats

Liu

Shi

et al. 2017

Oncotarget

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“…Since the combination of potassium oxonate with adenine was previously described in literature to induce the experimental model of uric acid nephropathy [31], we also used this model to investigate the synergistically proctective effects of Si-Wu-Tang and Er-Miao-San on hyperuricemia and renal impairment in rats. Moreover, adenine that is successfully used for hyperuricemia induction enters unmetabolized into the portal vasculature of the rat and therefore is metabolized in the liver [32] that will determine its impact on uricemia.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

Chinese Herbal Formulas Si-Wu-Tang and Er-Miao-San Synergistically Ameliorated Hyperuricemia and Renal Impairment in Rats Induced by Adenine and Potassium Oxonate

Guo

Jiang

Gui

et al. 2015

Cell Physiol Biochem

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Background/Aims: Hyperuricemia is an independent risk factor for chronic kidney disease and cardiovascular disease. Here, we examined the combined protective effects of Chinese herbal formula Si-Wu-Tang and Er-Miao-San on hyperuricemia and renal impairment in rats. Methods: Rats were randomly divided into normal rats, hyperuricemic rats, and hyperuricemic rats orally administrated with benzbromarone (4.5 mg·kg^-1·d^-1), Si-Wu-Tang (3.78 g·kg^-1·d^-1) and Si-Wu-Tang plus Er-Miao-San (6.48 g·kg^-1·d^-1) for 4 weeks. Hyperuricemic rats were orally gavaged with adenine (0.1 g·kg^-1·d^-1) and potassium oxonate (1.5 g·kg^-1·d^-1) daily for 4 weeks. Serum uric acid, creatinine, total cholesterol (TCH), triglyceride and blood urea nitrogen (BUN) concentrations, as well as urinary uric acid and microalbuminuria were measured weekly. Serum xanthine oxidase (XOD) activity and renal histopathology were also evaluated. The renal expression of organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) was detected by western blot. Results: Si-Wu-Tang plus Er-Miao-San lowered serum uric acid, creatinine, triglyceride and BUN levels to a greater degree than did Si-Wu-Tang alone. Si-Wu-Tang plus Er-Miao-San ameliorated microalbuminuria and renal histopathology, as well as decreased serum TCH concentration and XOD activity in hyperuricemic rats. Combination of Si-Wu-Tang and Er-Miao-San also led to a greater increase in OAT1 and OAT3 expression than did Siwutang alone. Conclusion: Si-Wu-Tang and Er-Miao-San synergistically ameliorated hyperuricemia and renal impairment in rats through upregulation of OAT1 and OAT3.

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“…Some Rubiaceae species have described biological properties, such as the species Uncaria tomentosa (Willd) D.C., popularly known as "unha-degato", being widely used in folk medicine for various indications: arthritis, asthma, cancer, gastric ulcer, inflammation and bleeding (Heitzman et al, 2005). Moreover, pharmacological studies also demonstrated that other species of this family have anti-inflammatory (Zhu et al, 2012), antinociceptive (Déciga-Campos et al, 2006), antibacterial (Comini et al, 2011), antitumor and antioxidant activities (Dreifuss et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatography-diode-array detector (HPLC-DAD) analysis and evaluation of antioxidant and photoprotective activities of extracts from seeds of Simira gardneriana M. R. V. Barbosa and Peixoto (Rubiaceae)

Ferraz¹,

Oliveira-JÃºnior²,

Oliveira³

et al. 2017

Afr. J. Biotechnol.

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Simira gardneriana is a Rubiaceae species commonly found in the Brazilian Northeast region, presented several therapeutic and biotechnological applications. In this paper, the antioxidant and photoprotective properties of extracts from the seeds of S. gardneriana were highlighted. The antioxidant activity of ethanol and methanol extracts (Si-EtOH and Si-MeOH, respectively) was determined, using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. The photoprotective activity of the extracts was evaluated using a spectrophotometric method. Total phenolic and flavonoid content was determined. In addition, a qualitative analysis of phytochemical markers and a high-performance liquid chromatography-diode-array detector (HPLC-DAD) analysis were also performed for both extracts. Concerning the antioxidant activity in vitro, Si-MeOH (EC 50 = 70.94±2.17 µg/ml) showed greater activity in comparison to Si-EtOH (EC 50 = 138.60±7.39 µg/ml). Once the sun protection factor spectrophotometric (SPF spectrophotometric ) of samples was calculated, it was demonstrated that the extracts show a similar photoprotective effect on all concentrations tested. SiEtOH and Si-MeOH tested on a concentration of 100 mg/l, exhibited SPF values equal to 3.37±0.006 and 3.36±0.007, respectively. HPLC fingerprints was recorded and showed the presence of characteristic peaks for phenolic compounds. The extracts showed significant phenols and flavonoids content according to the quantification methods used. According to the results, it was concluded that Si-EtOH and Si-MeOH have significant antioxidant and photoprotective activities. These activities are probably related to the profile of flavonoids and phenolic compounds found in this species.

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Anti-inflammatory and immunomodulatory effects of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats

Cited by 49 publications

References 22 publications

Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats

Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats

Chinese Herbal Formulas Si-Wu-Tang and Er-Miao-San Synergistically Ameliorated Hyperuricemia and Renal Impairment in Rats Induced by Adenine and Potassium Oxonate

High-performance liquid chromatography-diode-array detector (HPLC-DAD) analysis and evaluation of antioxidant and photoprotective activities of extracts from seeds of Simira gardneriana M. R. V. Barbosa and Peixoto (Rubiaceae)

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