Anti-inflammatory, antioxidant, cytotoxic activities, and sesquiterpenoid contents of Paralemnalia thyrsoides

Alarif, WaliedM; Alassass, AyaAli; Abubakr, MarwaS; Ayyad, Seif-EldinN; Mohammed, Abd-ElsalamE

doi:10.4103/pm.pm_222_21

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…As a result, drugs that may accelerate wound restoration with potential influence throughout each stage of the process, particularly those with low cost and fewer adverse effects, would be necessary for efficient therapy. P. thyrsoides red sea soft coral is one of the terpenoidrich soft corals with valuable biological activities, also, it is considered a member of the nardosinane-sesquiterpene producing family and possesses anti-inflammatory [60,61], cytotoxicity [16,62], neuroprotective [63], antifungal, and antibacterial activities [12,64]. So in the current study, we examined the antioxidant and wound healing effect of P. thyrsoides total extract, fractions, and metabolites in vivo, in vitro supported by histopathological and pharmacology network studies.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology, molecular docking study, and in vivo validation of the wound healing activity of the Red Sea soft coral Paralemnalia thyrsoides (Ehrenberg 1834) ethanolic extract and bioactive metabolites

el-dien,

Maher,

Hisham

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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Background Wounds are a major health issue on a global scale, putting a great deal of financial, commercial, and social strain on healthcare organizations, patients, and individuals. So, this study aims to investigate the in vitro antioxidant activity of Paralemnalia thyrsoides soft coral total ethanolic extract. Also, bio-guided in vivo wound healing validation enhanced by the evaluation of related gene expression of Paralemnalia thyrsoides total extract, derived fractions, and three known metabolites was done. Furthermore, utilizing network pharmacology, we identified ten hub target genes associated with wound healing, including AKT1(RAC-alpha serine/threonine–protein kinase), IL6 (interleukin-6), MAPK3 (mitogen-activated protein kinase 3), MMP9 (matrix metalloproteinase 9), and APP (amyloid P protein precursor). We conducted molecular docking to assess how the three compounds interact with these hub genes and inflammatory cytokines (IL-1β (interleukin-1 beta), TGF-β (transforming growth factor-beta), TNF-α (tumor necrosis factor-alpha), and NF-KB (nuclear factor-kappa B) linked to wound healing. Results In vitro antioxidant activity of the total ethanolic extract of Paralemnalia thyrsoides revealed potent scavenging activity against H2O2 with IC50 of 178.62 μg/mL. Additionally, the bio-guided scheme of the in vivo wound healing assay leads to the most active fraction, petroleum ether, with a healing percentage of 85% ± 4. Several chromatographic procedures upon petroleum ether fraction led to the isolation of three known compounds with significant in vivo wound healing potential which are recognized as triacontan-1-ol (1), 24-methylcholesterol (2) 6α-acetyl-7α-acetate-1(10)-α-13-nornardosine [C16H24O4] (3). Noteworthy downregulation in Cox-2 (Cyclooxygenase-2), Cox-1 (Cyclooxygenase-1), IL-1β, TGF-β, TNF-α, NF-KB, and INF-ϒ (interferon-ϒ) relative genes expression and upregulation in TGF-β, and IL-10 (interleukin-10) relative genes expression proved that compounds (3), (2), and (1) were, respectively, significant. The in silico study suggests that both C16H24O4 and 24-methyl cholesterol have potential in wound healing, possibly involving the regulation of RAC-alpha serine/threonine-protein kinase (AKT1). Conclusion Our study highlights the antioxidant and wound-healing potential of Paralemnalia thyrsoides soft coral that can be attributed to its diverse chemical metabolites. The in vivo and in silico findings highlighted that P. thyrsoides can be an effective remedy for wound restoration with the need for extensive future detailed clinical studies to prove these results.

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Section: Discussionmentioning

confidence: 99%

Network pharmacology, molecular docking study, and in vivo validation of the wound healing activity of the Red Sea soft coral Paralemnalia thyrsoides (Ehrenberg 1834) ethanolic extract and bioactive metabolites

el-dien,

Maher,

Hisham

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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“…Soft corals have unique and diverse structures, making them a rich source of natural products with potential medicinal applications [8]. Paralemnalia genus is one of the fascinating and important soft corals including 6 species widely distributed in tropical oceans and has been known to produce unique terpenoids with various biological activities [9]. Previous studies reported that Paralemnalia species have been found to exhibit various biological activities, indicating their potential as therapeutic agents [10].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies reported that Paralemnalia species have been found to exhibit various biological activities, indicating their potential as therapeutic agents [10]. Paralemnalia thyrsoides on which many studies have been conducted demonstrated various classes of secondary metabolites including sesquiterpenoids [11,12], diterpenes [13], and sterols [9] with promising biological activities such as anti-inflammatory [14], cytotoxicity [15], and antimicrobial [13]. Our study aimed to determine the chemical composition of the petroleum fraction of P. thyrsoides using GC-mass analysis to investigate its various metabolites from different classes.…”

Section: Introductionmentioning

confidence: 99%

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Chemical characterization of the petroleum ether fraction of Paralemnalia thyrsoides soft coral using Gas Chromatography-Mass analysis

Mohie el-dien,

Ahmed,

Khedr

et al. 2024

Journal of advanced Biomedical and Pharmaceutical Sciences

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Genus Paralemnalia (Family: Nephtheidae), specifically Paralemnalia thyrsoides, is a soft coral known for its unique morphology and potential medicinal applications. Commonly referred to as finger coral or tree coral, it has finger-like lobes branching from a fleshy base and is typically colored beige. These soft corals are particularly interesting due to the presence of various secondary metabolites. These chemicals are thought to be a defense mechanism against predators and may have other biological activities. Research has shown that extracts from Paralemnalia thyrsoides contain a variety of these metabolites, including new and previously known compounds. The present study indicates that the petroleum ether fraction of Paralemnalia thyrsoides contains various compounds that were investigated through gas chromatography-mass analysis (GC/MS). The most abundant compounds were saturated fatty acids methyl ester (22.46%), oxygenated compounds (19.98%), oxygenated sesquiterpenes (15.03%), unsaturated fatty acids methyl ester (10.06%), sterols (9.54%), nitrogenous compounds (7.33%), hydrocarbons (2.23%), in addition to minor saturated fatty alcohols (0.66%) and (12.71%) of the total compounds could not be identified based on the retention time and mass spectrum.

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“…The soft coral family Nephtheidae comprises 20 genera, which are a rich source of diverse and complex secondary metabolites, especially various types of terpenoids. , These metabolites present a wide range of biological properties, including cytotoxic, , antimicrobial, and anti-inflammatory activities. − Unlike other Nephtheidae genera such as Lemnalia , − Paralemnalia , − Litophyton , − and Nephthea , − soft corals belonging to the genus Stereonephthya have been less scrutinized, and only one species has been chemically investigated. Chemical investigation of S. crystalliana afforded nine steroids and steroid glycosides, with cytotoxicity activity …”

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confidence: 99%

Terpenoids from the Soft Coral Stereonephthya bellissima

Yu,

Han,

et al. 2024

J. Nat. Prod.

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A detailed chemical study of the extract from the soft coral Stereonephthya bellissima resulted in the isolation and identification of seven new sesquiterpenoids, bellissinanes A−G (1− 7), along with four new diterpenes (8−11). Bellissinane A (1) is the third reported nardosinane-type sesquiterpene bearing a 6/5/6 tricyclic system. Bellissinanes C and D (3, 4) contain a phenylethylamine fragment, which is relatively unusual in marine organisms. Bellissinanes E−G (5−7) belong to the rare class of nornardosinane sesquiterpenoids. Structurally uncommon octahydro-1H-indenyl-type and prenyleudesmane-type skeletons were characterized for herpetopanone B (8) and bellissimain A (9), respectively. Bellissinane E (5) exhibited in vivo angiogenesis-promoting activity.

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Anti-inflammatory, antioxidant, cytotoxic activities, and sesquiterpenoid contents of Paralemnalia thyrsoides

Cited by 6 publications

References 19 publications

Network pharmacology, molecular docking study, and in vivo validation of the wound healing activity of the Red Sea soft coral Paralemnalia thyrsoides (Ehrenberg 1834) ethanolic extract and bioactive metabolites

Network pharmacology, molecular docking study, and in vivo validation of the wound healing activity of the Red Sea soft coral Paralemnalia thyrsoides (Ehrenberg 1834) ethanolic extract and bioactive metabolites

Chemical characterization of the petroleum ether fraction of Paralemnalia thyrsoides soft coral using Gas Chromatography-Mass analysis

Terpenoids from the Soft Coral Stereonephthya bellissima

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