Anti-inflammatory, Antipyretic and Analgesic Activities of Ethanol Extract of Seriphidium kurramenses

Abstract

Seriphidium kurramenses traditionally medicinal plant usually not present in Pakistan is used in different biological activities to cure various ailments. However, scare studies are present on S. kurramenses regarding to its medicinal importance to treat fever, inflammation and pain. The purpose of present study is to investigate the anti-inflammatory, antipyretic and analgesic effects of S. kurramenses stem and leaves in ethanolic extract on albino rats. Methodi anti-inflammatory actions were evaluated by injecting carrageenan in rats which caused edema. S. kurramenses produced significant (P<0.005) reduction in edema at 200 and 50mg/kg doses with respective percentages (90, 81%) of leaf and stem extract as compared to standard (diclofenac, 90%) and control (normal saline, 100%) groups. Meanwhile, antipyretic activity was examined by inducing brewer’s yeast in albino rats which induced pyrexia. S. kurramenses showed significant reduction (P<0.005) at different doses of ethanolic extract (50mg/kg, 50mg/kg) with respective percentages of leaf (95%) and stem extract (100%) as compared to standard (brewer’s yeast 81%) and control groups (normal saline 100%). Similarly, analgesic test was assessed by injecting acetic acid which induced writhing movements and these movements were reduced (>0.005) at different doses of ethanolic extract (400mg/kg, 400mg/kg) with respective percentages of leaf and stem extracts (52%, 56%) as compared to standard (diclofenac, 48%) and control groups (100%). It is concluded that the extract of S. kurramenses exhibit significant (P<0.005) results in case of anti-inflammatory and antipyretic activities but insignificant (>0.005) in analgesic activity.

HPLC, FTIR and GC-MS Analyses of Thymus vulgaris Phytochemicals Executing In Vitro and In Vivo Biological Activities and Effects on COX-1, COX-2 and Gastric Cancer Genes Computationally

“…Different fractions were assessed for their thrombolytic activity by the method reported by Ramjan et al [ 59 ], and 500 μL of fresh human blood was added to already measured empty microcentrifuge tubes and incubated for 60 min at 37 °C. The serum was removed after incubation, and the weight was recalculated.…”

“…Streptokinase SK was used as the positive control, and deionized water was added as the negative control, and 100 μL of extracts and SK suspension were used for the in vitro analysis and added in incubated pre-weighted tubes and then again incubated for a further 90 min. After incubation, the serum was removed, weighed, and the lysis was calculated by the formula, with 100 μL of distilled water used as the negative control [ 59 ]: %Lysis = W C /W L × 100 W C = weight of lysis and W L = weight of clot …”

“…The temperature was noted at 0-h, 30-min, 60-min, and 120-min time intervals. The antipyretic activity was calculated by the given formula [ 59 ]: % Reduction = T − Cb/T − A × 100 where T = temperature after pyrexia, Cb = temperature of experimental groups 2–7, and A = normal body temperature.…”