Anti-inflammatory constituents from Perilla frutescens on lipopolysaccharide-stimulated RAW264.7 cells

Wang, Xiaofeng; Li, Heng; Jiang, Kun; Wang, Qi‐Qi; Zheng, Yu; Tang, Wei; Tan, Chen

doi:10.1016/j.fitote.2018.08.006

Cited by 48 publications

(28 citation statements)

References 25 publications

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“…From an integrated analysis of previous research [5,25,26], it could be inferred that lignans are the main non-volatile constituents with anti-inflammatory activity. Additionally, the flavonones are commonly found in the genus Asarum [9,55,57,72,73], but there is no study reporting whether the flavonones obtained from this genus possess anti-inflammatory activity since this type of compound had been proved to be potentially useful for inhibiting the inflammatory response [74,75]. This study demonstrated that two flavonones ( 29 and 30) showed anti-inflammatory activity against the release of β-glucuronidase in PMNs cells, which is the first report of the anti-inflammatory effects of flavonones from this genus.…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

et al. 2017

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Anti-inflammatory compounds were investigated from the ethanol extract of the roots and rhizomes of Asarum heterotropoides var. mandshuricum, a traditional Chinese medicine called Xixin and used for pain and inflammatory. Nine new compounds were isolated, including six new lignans, neoasarinin A–C (1–3), neoasarininoside A and B (4 and 5), and asarinin B (7), and one new monoterpene, asarincin A (8), two new amides, asaramid II and III (10 and 11), and one new natural monoterpene, asaricin B (9), along with 37 known compounds (6, 12–47). Their structures and absolute configurations were elucidated on the basis of spectroscopic methods and chemical analyses. This is the first report of the absolute configuration of asarinin A (6). The 8-O-4′ neolignans (1–5) were reported in the genus Asarum for the first time. The 15 compounds 17, 19, 22–25, 28, 31, 36, 40, 42, 43, 45–47 were isolated from the genus Asarum, and compounds 16, 32, 33, 37 and 39 were isolated from A. heterotropoides var. mandshuricum for the first time. Thirty-seven of the isolates were evaluated for anti-inflammatory activity against the release of β-glucuronidase in polymorphonuclear leukocytes (PMNs) induced by the platelet-activating factor (PAF), and compounds 1, 4, 7, 8, 14, 17–19, 22, 24, 25, 29, 30, 32, 33, 40–43, 45, and 46 showed potent anti-inflammatory activities in vitro, with 27.9%–72.6% inhibitions at 10−5 mol/L. The results of anti-inflammatory assay suggested that lignans obtained from the CHCl3 extract might be the main active components of Xixin.

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Section: Discussionmentioning

confidence: 99%

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

et al. 2017

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“…A recent study in mice showed that PF extract ameliorated inflammatory bowel disease (IBD) via protection of dextran sulfate sodium-induced murine colitis, with NF-κB and signal transduction and activator of transcription 3 (STAT3) as putative targets [95]. A perillaketone-type and alkaloid isolated from aerial parts of perilla showed the remarkable inhibitory effect on pro-inflammatory cytokines (TNF-α and/or IL-6) and inflammatory mediator (NO) in LPS-stimulated RAW264.7 cells, indicating that these compounds might be active components for inflammatory disorders [96].…”

Section: Pharmacological Properties Of Perillamentioning

confidence: 99%

Ethnomedicinal, Phytochemical and Pharmacological Investigations of Perilla frutescens (L.) Britt.

2018

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Perilla frutescens (L.) Britt. (PF) is an annual herbal medicinal, aromatic, functional food, and ornamental plant that belongs to the mint family, Lamiaceae. The origin of perilla traces back to East Asian countries (China, Japan, Korea, Taiwan, Vietnam, and India), where it has been used as a valuable source of culinary and traditional medicinal uses. The leaves, seeds, and stems of P. frutescens are used for various therapeutic applications in folk medicine. In the absence of a comprehensive review regarding all aspects of perilla, this review aims to present an overview pertaining to the botanical drug, ethnobotany, phytochemistry, and biological activity. It was found that the taxonomic classification of perilla species is quite confused, and the number of species is vague. Perilla has traditionally been prescribed to treat depression-related disease, anxiety, asthma, chest stuffiness, vomiting, coughs, colds, flus, phlegm, tumors, allergies, intoxication, fever, headache, stuffy nose, constipation, abdominal pain, and indigestion, and acts as an analgesic, anti-abortive agent, and a sedative. Until now, 271 natural molecules have been identified in perilla organs including phenolic acids, flavonoids, essential oils, triterpenes, carotenoids, phytosterols, fatty acids, tocopherols, and policosanols. In addition to solvent extracts, these individual compounds (rosmarinic acid, perillaldehyde, luteolin, apigenin, tormentic acid, and isoegomaketone) have attracted researchers’ interest for its pharmacological properties. Perilla showed various biological activities such as antioxidant, antimicrobial, anti-allergic, antidepressant, anti-inflammatory, anticancer, and neuroprotection effects. Although the results are promising in preclinical studies (in vitro and in vivo), clinical studies are insufficient; therefore, further study needs to be done to validate its therapeutic effects and to ensure its safety and efficacy.

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“…In ammation is a natural biological response to injury or infection in the human body. Inhibition of the production of in ammatory mediators, such as NO, and in ammatory cytokines, such as tumor necrosis factor α,interleukin 6, and interleukin 1β, serves as a key mechanism in controlling in ammation [27]. In this study, the in ammatory mediator NO was used to test of the anti-in ammation activity of the extracted polysaccharides.…”

Section: Cytotoxicity and Anti-in Ammatory Activities Of The Polysaccmentioning

confidence: 99%

Structural Characterization of Water-Soluble Polysaccharides from Sophora flavescensAit. and Their Anti-Inflammatory Evaluation Based on NO Release

Linghu

Jiang

et al. 2020

Preprint

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Background: The dried roots of Sophora flavescens Ait. are traditionally used as Sophora Flavescens (Kushen in Chinese) to treat inflammatory diseases. It is traditionally served as a decoction, and polysaccharides represent one the major chemical constituents of this decoction. How about the structure of S. flavescens polysaccharides and whether they have anti-inflammatory activity should be uncovered.Methods: The purified polysaccharides were isolated through a combination of ion-exchange chromatography on DEAE 650 M and gel filtration on Superdex G-200 from hot water extract of S. flavescens. Structure was characterized by chemical derivatization as well as HPLC, FT-IR, GC-MS and NMR technologies. The preliminary in vitro antiinflammation activity was tested on RAW 264.7 cells upon NO release inhibition.Results: In this study, four polysaccharides, namely, SFNP-1, SFNP-2, SFAP-1, and SFAP-2, were isolated from S. flavescens. Results showed that both SFNP-1 and SFNP-2 contained (1→4)-linked glucans with small amounts of side chains at the O-4 position of the backbone chain residues. The two acidic polysaccharides (i.e.,SFAP-1 and SFAP-2) were identified to be pectin-type polysaccharides mainly containing a homo-galacturanan backbone consisting of α-(1→4)-linked GalAp and methyl-esterified α-(1→4)-linked GalAp residues at a ratio ofapproximately1:1. The bioactivity test revealed that the four purified polysaccharides have no cytotoxicity on RAW264.7andthat SFNP-1 and SFNP-2 show significant stimulating activity. Although the decoction of S. flavescens has been traditionally used as an anti-inflammatory agent, NO release inhibition results showed thatSFAP-1 and SFAP-2, as the major polysaccharides of SFCP, do not have significant anti-inflammatory effects. Conclusions: This result suggests that the anti-inflammatory effect of the decoction of S. flavescens may depend on the presence of alkaloids and not the polysaccharides it contains.

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Anti-inflammatory constituents from Perilla frutescens on lipopolysaccharide-stimulated RAW264.7 cells

Cited by 48 publications

References 25 publications

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

Ethnomedicinal, Phytochemical and Pharmacological Investigations of Perilla frutescens (L.) Britt.

Structural Characterization of Water-Soluble Polysaccharides from Sophora flavescensAit. and Their Anti-Inflammatory Evaluation Based on NO Release

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