Anti-Inflammatory Cytokines in Peri-Implant Soft Tissues: A Preliminary Study on Humans Using CDNA Microarray Technology

Bambini, Fabrizio; Pellecchia, M.; Memé, Lucia; Santarelli, Andrea; Emanuelli, Monica; Procaccini, Maurizio; Muzio, Lorenzo Lo

doi:10.1177/1721727x0700500302

Cited by 9 publications

(9 citation statements)

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“…Bone resorption in edentulous regions often results in an inadequate ridge for implant osseointegration (10). In order to overcome this problem, different bone-grafting procedures and materials have been used to provide a correct implant placement.…”

mentioning

confidence: 99%

“…Since estrogenic therapy (5), several studies have demonstrated that this drug prevents post-menopausal bone loss (2, 3), a new was able to maintain a constant level of bone min-eralization (6,7), having a protective effect on bone density similar to estrogens (8) without the suppressive effects on bone formation index (9). Bone resorption in edentulous regions often results in an inadequate ridge for implant osseointegration (10). In order to overcome this problem, different bone-grafting procedures and materials have been used to provide a correct implant placement.…”

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confidence: 99%

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MG63 and MC3T3-E1 Osteoblastic Cell Lines Response to Raloxifene

et al. 2013

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Bone resorption in edentulous regions often results in inadequate ridge for implant osseointegration. In order to overcome this problem, the use of osteoconductive biomaterials has been proposed as a carrier for different types of pharmacological molecules. Since raloxifene, a drug used in osteoporosis therapy, inhibits the osteoclast, but not osteoblast functions, it has been suggested to improve recovery during implant surgery. The present work evaluated in vitro the effect of raloxifene on two different cell populations: the human osteoblast-like cells (MG63) and osteoblasts derived from rat calvaria (MC3T3-El). The morpho-functional investigations carried out showed a different behavior of the two cell lines. Raloxifene showed a stimulatory effect towards MG63 cell proliferation with a significant increase in cell viability after 7 days of culture. On the contrary, MC3T3-El cells showed a significant reduction in cell viability, when compared with the same cells at 72 h, or with the control cell population. The predominantly proliferative effect of raloxifene on MG63 cells is partly confirmed by the reduction of alkaline phosphatase activity, an early marker of osteoblast differentiation. The different effect of raloxifene on osteoblastic population in relationship to the type and age of the cell is an issue that needs further investigation.The physiological process of bio-mineralization class of compounds called SERMs (Selective Estroin bone tissue is a dynamic process, orchestrated by gen Receptor Modulators), to which raloxifene becomplex biological systems. There are many biolongs, was developed to avoid well-known adverse chemical agents, such as estrogen and estrogen-like effects of estrogens. In fact, SERMs exert selective molecules, that can improve bone formation and agonist or antagonist effects, activating estrogen remineralization, induce osteoblastic differentiation, ceptors only in target tissues (4). Since Black et al. or determine the release of growth factors important established raloxifene anti-osteopenic potentiality for bone regeneration (1). Since estrogenic therapy (5), several studies have demonstrated that this drug prevents post-menopausal bone loss (2, 3), a new was able to maintain a constant level of bone min-

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MG63 and MC3T3-E1 Osteoblastic Cell Lines Response to Raloxifene

et al. 2013

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“…Since lack of published reference values prevents from discriminating which outliers represent errors or an actual enhanced response in some individuals, outliers were not discarded opting for a non-parametric test, which is least influenced by outliers, for data analysis. Lastly, since our study focused on CRP changes, the overall status of the inflammation response following ACL surgery has not been exhaustively described (17)(18)(22)(23).…”

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confidence: 99%

“…ESR is elevated when a local or systemic inflammation is present regardless of its cause. CRP is an acute-phase plasma protein increasing in bacterial infection and extensive tissue damage (17)(18). It is analysed in venous blood collected in a serum-separating tube according to various methods.…”

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confidence: 99%

Clinical and Laboratory Changes in the Uncomplicated Course of Arthroscopic Anterior Cruciate Ligament (ACL) Reconstruction: A Prospective Observational Study in 58 Patients

Calvisi¹,

Lupparelli²,

Rossetti³

et al. 2008

Eur J Inflamm

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Knowledge of post-operative clinical and laboratory changes in the uncomplicated course of ACL reconstruction could assist orthopaedic surgeons in making a timely diagnosis of septic arthritis. A total of 58 candidates to ACL reconstruction were enrolled in this study according to inclusion/exclusion criteria. Pre-and post-operative changes on I", 3 rd , T", 15 th, and 30 t h day were collected for skin body temperature (SBT), knee pain (KP), white blood cell (WBC) count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP). Post-operative joint effusion was recorded when inhibiting quadriceps contraction. The post-operative changes were compared with baseline values at each time point in the whole sample and in the subgroups of patients receiving bone-patellar-tendon-bone (BPTP) or hamstring tendon (HT) autograft. Follow-up at six month was considered adequate to rule out surgery-related septic arthritis. Mean SBT significantly increased on I", 3 r d and 7 th post-operative day and returned to values not differing from baseline on 15 t h and 30 t h day. Median KP was significantly elevated during the first month. Joint effusion was observed on 7 t h post-operative day in 52% of patients. Mean WBC count significantly increased on 1'1, T" and 15 t h day while no difference from baseline was observed on 3 rd and 30 th day. Mean ESR was significantly elevated on I" day, increased on 3 rd day and peaked on 7 th day. ESR decreased on 15 t h and 30 th day but remained significantly higher than baseline. Mean CRP significantly increased on 1st day, peaked on 3 rd day and decreased on 7 t h day, while levels on 15 th and 30 th day did not differ from baseline. The SBT, KP, WBC count, ESR and CRP changes stratified in relation to the type of autograft showed the same variation trend as in the whole sample. Close clinical surveillance may be advisable when SBT, occurrence of joint effusion and CRP levels deviate from the described reference trend two weeks after surgery.Although the natural history of ACL-insufficient knees is unclear (1-2), it is generally agreed that recurrent episodes of symptomatic instability would lead to an accrual ofjoint damage (l , 3), particularly if a meniscectomy has been performed following ACLtear (4). The indication for ACL reconstruction includes the desire of recreational or professional athletes to return promptly to the pre-injury activity level or the persistence of symptomatic instability following rehabilitation and activity level modifications (l, 3).The onset of septic arthritis, the reported rate of

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“…These studies urged to test the coupling of other kinds of biomaterials acting as a carrier for drugs (8). Such attempts have already been made using biomaterials associated with bisphosphonates (9).…”

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Novel Hydroxyapatite Biomaterial Covalently Linked to Raloxifene

Memé

Santarelli

Marzo

et al. 2014

Int J Immunopathol Pharmacol

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Since raloxifene, a drug used in osteoporosis therapy, inhibits osteoclast, but not osteoblast functions, it has been suggested to improve recovery during implant surgery. The present paper describes an effective method to link raloxifene, through a covalent bond, to a nano-Hydroxyapatite-based biomaterial by interfacing with (3-aminopropyl)-Triethoxysilane as assessed by Infra Red-Fourier Transformed (lR-FT) spectroscopy and Scanning Electron Microscope (SEM). To evaluate the safety of this modified new material, the vitality of osteoblast-like ceUs cultured with the new biomaterial was then investigated. Raloxifene-conjugated HAbiomaterial has been shown to be a safe material easy to obtain which could be an interesting starting point for the use of a new functional biomaterial suitable in bone regeneration procedures.

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Anti-Inflammatory Cytokines in Peri-Implant Soft Tissues: A Preliminary Study on Humans Using CDNA Microarray Technology

Cited by 9 publications

References 30 publications

MG63 and MC3T3-E1 Osteoblastic Cell Lines Response to Raloxifene

MG63 and MC3T3-E1 Osteoblastic Cell Lines Response to Raloxifene

Clinical and Laboratory Changes in the Uncomplicated Course of Arthroscopic Anterior Cruciate Ligament (ACL) Reconstruction: A Prospective Observational Study in 58 Patients

Novel Hydroxyapatite Biomaterial Covalently Linked to Raloxifene

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