Anti-inflammatory effect and prostate gene expression profiling of steryl ferulate on experimental rats with non-bacterial prostatitis

Abstract

Steryl ferulate (SF) is a bioactive mixture extracted from rice bran and shows higher inhibitory activity against inflammation than the corresponding free sterols. In this study, the aim was to investigate the anti-inflammatory effect and prostate gene expression profiling of SF using a Xiaozhiling-induced non-bacterial prostatitis (NBP) rat model. The anti-inflammatory effect was evaluated by prostate weight, prostate index, acid phosphatase, density of lecithin corpuscles (DLC), white blood cell count (WBC), and prostatic histologic section. Prostate gene expression profiling was assessed by a cDNA microarray and validated by quantitative real-time PCR of five selected genes. Pathway analysis and Gene ontology (GO) analysis were applied to determine the roles of these differentially expressed genes involved in these biological pathways or GO terms. SF treatment could significantly inhibit prostate weight, prostate index, total acid phosphatase, prostatic acid phosphatase and WBC, suppress the severity of histological lesion and increase the DLC. Compared with the control group, the SF treatment group contained 238 up-regulated genes and 111 down-regulated genes. GO analysis demonstrated that the most significant expression genes were closely related to the terms of fibrinolysis, inflammatory response, high-density lipoprotein particle, protein-lipid complex, enzyme inhibitor activity, peptidase inhibitor activity and others. Canonical pathway analysis indicated five pathways were significantly regulated, which were associated with inflammation and tumorgenesis. In conclusion, SF may be used as a health supplement to prevent NBP, in that it could inhibit prostate inflammation in NBP patients by affecting the expression of genes in the related GO terms and pathways.

Differentiation of rice varieties using small bioactive lipids as markers

“…γ‐Oryzanol, a mixture of steryl ferulates (esters of plant sterols with ferulic acid), has gained great interest due to its bioactive properties. In various studies, γ‐oryzanol has been reported with cholesterol lowering , antioxidant , anti‐inflammatory , and anti‐carcinogenic effects , which are responsible for the health benefits of the whole rice grain. The varying profiles in bioactive lipids within rice may suggest different health benefits of rice.…”

The impact of SOD3 on prostatic diseases: elevated SOD3 is a novel biomarker for the diagnosis of chronic nonbacterial prostatiti

“…The experimental animals were divided into 2 groups: saline group and xiaozhiling group. Following abdominal surgery, the prostate was exposed and injected with 0.1 ml of normal saline or an equal volume of xiaozhiling respectively as described (22). One month later, rats were anesthetized with intraperitoneal injection of pentobarbital sodium, and prostate tissue was excised by abdominal surgery.…”

The Impact of SOD3 on Prostatic Diseases: Elevated SOD3 Serves as a Novel Biomarker for the Diagnosis of Chronic Nonbacterial Prostatitis

“…The experimental animals were randomly divided into 2 groups: saline group and xiaozhiling group. Following abdominal surgery, the prostate was exposed and injected with 0.1 ml of normal saline or an equal volume of xiaozhiling respectively as described [22]. One month later, rats were anesthetized with intraperitoneal injection of pentobarbital sodium, and the prostate tissue was excised by abdominal surgery.…”