Anti-inflammatory Effect of (7R,8S)-dehydrodiconiferyl Alcohol-92 A-methyl Ether from the Rhizome of Belamcanda Chinensis: Role of miR-146a and miR-155

Binh, Bui Thi; Hien, Tran Thi; Ha, Do Thi; Chinh, Pham Duc; Dung, Le Viet; Thu, Nguyen Thi Bich

doi:10.13005/bpj/1029

Cited by 2 publications

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“…Furthermore, Cox-1 is shown in angiogenesis in endothelial cells. Cox-2 is a well-known gene associated with inflammatory mediation and participates in numerous biological processes such as pain, inflammation, cancer, angiogenesis, carcinogenesis, or the development of immunity [ 63 ]. According to Cheng, Zhao, Ke, Wang, Cao, Liu, He and Rong [ 64 ], the inhibition of miR-155 and Cox-2 provides a protective effect in high-glucose conditions [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…MiR-155 enhances Cox-2 expression and is an established regulator of epithelial–mesenchymal transition and inflammation [ 65 ]. Some natural compounds suppress inflammatory activity, especially those that are found in traditional medicine and dietary supplements, which have the potential to be developed as a Cox-2 inhibitor [ 63 ]. Cox-1 expression increased post-treatment HAART–SP in this present study and is a sign of a protective function and successful synergy between SP and HAART ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

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Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

Sibiya

Ghazi

Mohan

et al. 2022

Plants

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The introduction of highly active antiretroviral therapy (HAART) in the treatment of HIV/AIDS has recently gained popularity. In addition, the significant role of microRNA expression in HIV pathogenesis cannot be overlooked; hence the need to explore the mechanisms of microRNA expression in the presence of HAART and Spirulina platensis (SP) in HepG2 cells. This study investigates the biochemical mechanisms of microRNA expression in HepG2 cells in the presence of HAART, SP, and the potential synergistic effect of HAART–SP. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine cell viability following SP treatment. The cellular redox status was assessed using the quantification of intracellular reactive oxygen species (ROS), lipid peroxidation, and a lactate dehydrogenase (LDH) assay. The fluorometric JC-1 assay was used to determine mitochondrial polarisation. The quantitative polymerase chain reaction (qPCR) was also employed for micro-RNA and gene expressions. The results show that MiR-146a (p < 0.0001) and miR-155 (p < 0.0001) levels increased in SP-treated cells. However, only miR-146a (p < 0.0001) in HAART–SP indicated an increase, while miR-155 (p < 0.0001) in HAART–SP treatment indicated a significant decreased expression. Further inflammation analysis revealed that Cox-1 mRNA expression was reduced in SP-treated cells (p = 0.4129). However, Cox-1 expression was significantly increased in HAART–SP-treated cells (p < 0.0001). The investigation revealed that HepG2 cells exposed to HAART–SP treatment showed a significant decrease in Cox-2 (p < 0.0001) expression. mRNA expression also decreased in SP-treated cells (p < 0.0001); therefore, SP potentially controls inflammation by regulating microRNA expressions. Moreover, the positive synergistic effect is indicated by normalised intracellular ROS levels (p < 0.0001) in the HAART–SP treatment. We hereby recommend further investigation on the synergistic roles of SP and HAART in the expression of microRNA with more focus on inflammatory and oxidative pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

Sibiya

Ghazi

Mohan

et al. 2022

Plants

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Dehydrodiconiferyl alcohol from Silybum marianum (L.) Gaertn accelerates wound healing via inactivating NF-κB pathways in macrophages

Qin

Xue

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives The aim of this study was to investigate the molecular mechanisms of the efficacy of lignin compound dehydrodiconiferyl alcohol (DHCA) isolated from Silybum marianum (L.) Gaertn in improving wound healing. These findings preliminarily brought to light the promising therapeutic potential of DHCA in skin wound healing. Methods First, the effect of DHCA on healing in vivo was studied using a full-thickness scalp wound model of mice by topical administration. Histopathological examinations were then conducted by haematoxylin and eosin (H&E), Masson’s trichrome staining and the immunofluorescence assay. Second, we further examined the anti-inflammatory mechanism of DHCA in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by immunofluorescence assay and Western blot analysis. Key findings DHCA could promote scalp wound healing in mice by enhancing epithelial cell proliferation and collagen formation and reducing inflammatory cells infiltration. Moreover, the NF-κB nuclear translocation was suppressed remarkably by DHCA administration in connective tissue of healing area. DHCA was also shown to inhibit production of nitric oxide (NO) and interleukin (IL)-1β with downregulated inducible nitric oxide synthase (iNOS) expression in LPS-induced RAW 246.7 cells. More importantly, DHCA administration upregulated p-IκBα expression and induced nuclear translocation of NF-κB without affecting its expression. Conclusions Our study indicated that DHCA exerted anti-inflammatory activity through inactivation of NF-κB pathways in macrophages and subsequently improved wound healing.

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Anti-inflammatory Effect of (7R,8S)-dehydrodiconiferyl Alcohol-92 A-methyl Ether from the Rhizome of Belamcanda Chinensis: Role of miR-146a and miR-155

Cited by 2 publications

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Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

Dehydrodiconiferyl alcohol from Silybum marianum (L.) Gaertn accelerates wound healing via inactivating NF-κB pathways in macrophages

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