Anti-inflammatory Effect of a Combination of Cannabidiol and<i>Morinda citrifolia</i>Extract on Lipopolysaccharide-stimulated RAW264 Macrophages

Tanikawa, Takashi; Kitamura, Masashi; Hayashi, Yasuhiko; Tomida, Natsumi; Uwaya, Akemi; Isami, Fumiyuki; Yokogawa, Takami; Inoue, Yutaka

doi:10.21873/invivo.13117

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…This combination was found to be more effective at inhibiting nitric oxide production and reducing iNOS expression than CBD alone. These findings suggest the therapeutic potential of CBD in the treatment of neuroinflammatory conditions and highlight its synergistic effect with other compounds in modulating inflammation [83]. Similarly, a study on orofacial dyskinesia in mice, induced with haloperidol, investigated the effects of CBD.…”

Section: Role Of Cannabis Compounds In Neurological and Systemic Infl...mentioning

confidence: 82%

Possible Role of Cannabis in the Management of Neuroinflammation in Patients with Post-COVID Condition

Cárdenas-Rodríguez,

Ignacio-Mejía,

Correa-Basurto

et al. 2024

IJMS

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The post-COVID condition (PCC) is a pathology stemming from COVID-19, and studying its pathophysiology, diagnosis, and treatment is crucial. Neuroinflammation causes the most common manifestations of this disease including headaches, fatigue, insomnia, depression, anxiety, among others. Currently, there are no specific management proposals; however, given that the inflammatory component involves cytokines and free radicals, these conditions must be treated to reduce the current symptoms and provide neuroprotection to reduce the risk of a long-term neurodegenerative disease. It has been shown that cannabis has compounds with immunomodulatory and antioxidant functions in other pathologies. Therefore, exploring this approach could provide a viable therapeutic option for PCC, which is the purpose of this review. This review involved an exhaustive search in specialized databases including PubMed, PubChem, ProQuest, EBSCO, Scopus, Science Direct, Web of Science, and Clinical Trials. Phytocannabinoids, including cannabidiol (CBD), cannabigerol (CBG), and Delta-9-tetrahydrocannabinol (THC), exhibit significant antioxidative and anti-inflammatory properties and have been shown to be an effective treatment for neuroinflammatory conditions. These compounds could be promising adjuvants for PCC alone or in combination with other antioxidants or therapies. PCC presents significant challenges to neurological health, and neuroinflammation and oxidative stress play central roles in its pathogenesis. Antioxidant therapy and cannabinoid-based approaches represent promising areas of research and treatment for mitigating adverse effects, but further studies are needed.

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Section: Role Of Cannabis Compounds In Neurological and Systemic Infl...mentioning

confidence: 82%

Possible Role of Cannabis in the Management of Neuroinflammation in Patients with Post-COVID Condition

Cárdenas-Rodríguez,

Ignacio-Mejía,

Correa-Basurto

et al. 2024

IJMS

View full text Add to dashboard Cite

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“…Numerous publications have demonstrated the potent anti-inflammatory effect of CBD both in-vitro and in-vivo [ 13 , 57 , 58 ]. The underlying mechanisms involve direct suppression of the activation of different types of immune cells, induction of apoptosis, and promotion of regulatory cells, which in turn control other immune cell targets.…”

Section: Discussionmentioning

confidence: 99%

Effects of combined cannabidiol (CBD) and hops (Humulus lupulus) terpene extract treatment on RAW 264.7 macrophage viability and inflammatory markers

Dammann,

Keil,

Hardewig

et al. 2023

Nat. Prod. Bioprospect.

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This study investigates the potential of cannabidiol (CBD), one major cannabinoid of the plant Cannabis sativa, alone and in combination with a terpene-enriched extract from Humulus lupulus (“Hops 1”), on the LPS-response of RAW 264.7 macrophages as an established in vitro model of inflammation. With the present study, we could support earlier findings of the anti-inflammatory potential of CBD, which showed a dose-dependent [0–5 µM] reduction in nitric oxide and tumor necrosis factor-alpha (TNF-α) released by LPS-stimulated RAW 264.7 macrophages. Moreover, we observed an additive anti-inflammatory effect after combined CBD [5 µM] and hops extract [40 µg/mL] treatment. The combination of CBD and Hops 1 showed effects in LPS-stimulated RAW 264.7 cells superior to the single substance treatments and akin to the control hydrocortisone. Furthermore, cellular CBD uptake increased dose-dependently in the presence of terpenes from Hops 1 extract. The anti-inflammatory effect of CBD and its cellular uptake positively correlated with terpene concentration, as indicated by comparison with a hemp extract containing both CBD and terpenes. These findings may contribute to the postulations for the so-called “entourage effect” between cannabinoids and terpenes and support the potential of CBD combined with phytomolecules from a non-cannabinoid source, such as hops, for the treatment of inflammatory diseases.

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“…[40][41][42] In addition, many studies have indicated that LPS could alter iNOS and COX-2 expression in RAW264.7 cells. 43,44 The reduction of iNOS and COX-2 expression in LPS-induced RAW264.7 is therefore considered to be an effective first step in determining the effectiveness of potential therapies for the treatment of inflammatory diseases, especially AD.…”

Section: Discussionmentioning

confidence: 99%

The Investigation of The Network Pharmacology and Mechanism of Action of Centella Asiatica Extract on The Atopic Dermatitis Model

Tungsukruthai,

Pochairach,

Krajarng

et al. 2023

Pharmacogn J.

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Background: Atopic dermatitis (AD) is a chronic relapsing inflammatory skin condition which has a negative impact on children health. The well-known medicinal plant Centella asiatica extract (CE) is used in herbal skin care products to produce various pharmacological effects in dermatology. However, the molecular target of CE in suppressing inflammatory is largely unknown. Objective: the aim of this study was to examine anti-inflammatory properties and network pharmacology of CE in lipopolysaccharide (LPS)induced AD in vitro model. Method: RAW264.7 cells were pre-treated with CE and then were stimulated with LPS and then were investigated cell viability, NO production, and the levels of pro-inflammatory mediators. In addition, the Search Tool for Retrieval of Interacting Genes (STRING), SwissTargetPrediction and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to construct the defined mechanism of action and network pharmacology. Results: CE showed the potent inhibitory effects on LPS-induced NO. In addition, CE significantly suppressed the expression of iNOS and COX-2, as well as the production of IL-2, IL-6, IL-10, and TNFα. Furthermore, the network pharmacological analysis revealed the potential role of CE in biological processes such as regulating JAK/STATs pathway and inhibiting proinflammatory cytokines both of which were linked to AD pathogenesis. Conclusion: Our findings confirm our hypothesis that CE could be developed as a therapeutic therapy for atopic dermatitis due to its pharmacological action and signaling mechanism in the modulation of allergic skin inflammation.

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Anti-inflammatory Effect of a Combination of Cannabidiol andMorinda citrifoliaExtract on Lipopolysaccharide-stimulated RAW264 Macrophages

Cited by 4 publications

References 29 publications

Possible Role of Cannabis in the Management of Neuroinflammation in Patients with Post-COVID Condition

Possible Role of Cannabis in the Management of Neuroinflammation in Patients with Post-COVID Condition

Effects of combined cannabidiol (CBD) and hops (Humulus lupulus) terpene extract treatment on RAW 264.7 macrophage viability and inflammatory markers

The Investigation of The Network Pharmacology and Mechanism of Action of Centella Asiatica Extract on The Atopic Dermatitis Model

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