Anti‐inflammatory effect of a polysaccharide fraction from Craterellus cornucopioides in LPS‐stimulated macrophages

Abstract

Immunocytes‐involved inflammation is considered to modulate the damage in various diseases. Oxidative stress is initiated by oxidative agents such as LPS and ROS, which are strongly involved in chronic inflammation. Our previous study found that a polysaccharide fraction from Craterellus cornucopioides (CCPP‐1) showed good antioxidant activity. However, the anti‐inflammatory effect of CCPP‐1 was still elusive. The objective of this study was to evaluate the anti‐inflammatory activity of CCPP‐1 and its potential mechanism in LPS‐stimulated RAW264.7 macrophages. The results showed that CCPP‐1 could inhibit LPS‐induced ROS and NO accumulation. Additionally, CCPP‐1 could decrease pro‐inflammatory cytokines production (TNF‐α, IL‐1β, and IL‐18) and inflammatory mediator (iNOS) expression, which might be associated with its capacity to inhibit NF‐κB signaling pathway and NLRP3 inflammasome activation. Therefore, this study suggested that CCPP‐1 had an ameliorative effect on the inflammation response and was potential to develop into functional food for treating chronic inflammation. Practical applications Craterellus cornucopioides is an edible fungus widely distributed in Southwestern China. It was reported that C. cornucopioides polysaccharide (CCPP‐1), as important active ingredient, showed good antioxidant activity. However, the anti‐inflammatory effect was still elusive. This study showed that CCPP‐1 possessed anti‐inflammatory activity. The molecular mechanism might be associated with its capacity to inhibit NF‐κB signaling pathway and NLRP3 inflammasome activation. Therefore, polysaccharides from C. cornucopioides have potential to develop into functional food to combat inflammatory condition and thus indirectly halt the progression of various inflammatory response‐related chronic diseases.

Comprehensive Review of Functional and Nutraceutical Properties of Craterellus cornucopioides (L.) Pers.

“…Together with phenolic compounds, polysaccharides are the most cited compounds in the composition of C. cornucopioides aqueous extracts. CCPs-1 and CCPs-2 are two water-soluble acidic polysaccharides with different contents of uronic acid, rhamnose, fucose, arabinose, xylose, mannose, glucose, and galactose [ 13 , 14 , 18 , 24 , 25 , 26 , 27 , 28 ].…”

A network pharmacology strategy combined with in vitro experiments to investigate the potential anti‐inflammatory mechanism of Prunus cerasifera Ehrhart

“…Activated macrophages release a large number of cytokines and pro‐inflammatory mediators, which cause oxidative stress, and finally lead to the occurrence of acute and chronic inflammatory diseases (Xu et al, 2021). The protein expression levels of pro‐inflammatory cytokines TNF‐ α , TGF‐ β , IL‐1 β , and IL‐6 were estimated by ELISA analysis to further study the anti‐inflammatory effects of EHP on LPS‐treated RAW264.7 cells.…”

Structure, function and mechanism of edible fungus polysaccharides in human beings chronic diseases

“…In terms of anti-inflammatory, Sheng et al (2021) found that Lentinus edodes polysaccharides have antiinflammatory effects. Additionally, Craterellus cornucopioides polysaccharides showed anti-inflammatory effect by reducing the production of inflammatory mediators (iNOS) and the expression of pro-inflammatory factors (TNF-α, IL-1β and IL-18), which may be related to the inhibition of NF-κB signaling pathway (Xu et al, 2021a). For hyperlipidemia, Yu et al (2021) confirmed that Cordyceps militaris polysaccharides can alleviate hyperlipidemia by blocking preadipocyte differentiation.…”