Anti-inflammatory effect of Amomum xanthioides in a mouse atopic dermatitis model

Abstract

Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disorder. The present study investigated the effects of Amomum xanthioides extract (AXE) on AD‑like skin inflammation using a Dermatophagoides farinae extract (DFE) and 2,4‑dinitrochlorobenzene (DNCB)‑induced mouse AD model. Hematoxylin and eosin staining results demonstrated that repeated DFE/DNCB exposure markedly increased the thickening of the dermis and epidermis, in addition to the infiltration of eosinophils and mast cells. However, oral administration of AXE reduced these histopathological alterations in a dose‑dependent manner. Elevated serum histamine, total and DFE‑specific immunoglobulin E (IgE), and IgG2a were also decreased by treatment with AXE. In addition, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) results demonstrated that the mRNA expression of tumor necrosis factor (TNF)‑α, interferon (IFN)‑γ, interleukin (IL)‑4, IL‑13, IL‑31 and IL‑17A was reduced in ear skin following AXE administration in AD mice. Fluorescence‑activated cell sorting demonstrated that the population of CD4+/IL‑4+, CD4+/IFN‑γ+ and CD4+/IL‑17A+ cells in draining lymph nodes was also significantly decreased in AXE‑treated mice compared with AD mice without AXE treatment. Furthermore, keratinocytes that were stimulated with TNF‑α and IFN‑γ exhibited increased gene expression of pro‑inflammatory cytokines and chemokines, including TNF‑α, IL‑1β, IL‑6, IL‑8, C‑C motif chemokine ligand (CCL)17 and CCL22, as determined by RT‑qPCR. However, upregulation of these genes was reduced by AXE pretreatment. Based on these results, we hypothesize that AXE may be useful in the treatment of allergic skin inflammation, particularly AD.

Ameliorative effects of green tea extract from tannase digests on house dust mite antigen-induced atopic dermatitis-like lesions in NC/Nga mice

“…In general, AD is an immunological disease resulting from an imbalance favoring type-2 helper T (Th2) cell. Th2 cells, mast cells, or other immune components cells produce interleukin-4 (IL-4), which is known to induce B-cell class switching to IgE [7, 26, 29]. Up-regulation of IgE production is a hallmark of AD in humans [15].…”

Anti-Inflammatory Effect of Chloroform Fraction of Pyrus Ussuriensis Maxim. Leaf Extract on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis in nc/nga Mice

“…During the inflammatory process, primary inflammatory cytokines TNF-α and IL-1β activate the production of secondary inflammatory cytokines like IL-6, IL-8, IL-12, IL-15, IL-17, and IL-18 [29]. TNF-α and IFN-γ stimulation in keratinocytes has been reported to induce inflammatory cytokines and chemokines [30,31]. Thus, inhibiting these inflammatory cytokines is an important target in reducing inflammation.…”

Anti-Inflammatory Effects of Essential Oils of Amomum aromaticum Fruits in Lipopolysaccharide-Stimulated RAW264.7 Cells

“…e results showed that the volatile oils exhibited moderate activities compared with standard drug, diclofenac [14]. e extracts of A. compactum, A. xanthoides, and A. vilosum also demonstrated their anti-inflammatory activities in vitro and in vivo [28][29][30]. Interestingly, there have been very few studies on the Amomum essential oils with anti-inflammation.…”