Anti-inflammatory effect of conditioned medium from human uterine cervical stem cells in uveitis

Bermúdez, María A.; Sendón-Lago, Juan; Seoane, Samuel; Eiró, Noemí; González, Francisco; Saá, Jorge; Vizoso, F; Perez-Fernandez, Román

doi:10.1016/j.exer.2016.06.022

Cited by 78 publications

(64 citation statements)

References 27 publications

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“…Moreover, in vitro next-generation sequencing confirmed the increase in the genes involved in ossification (ASF1A, GDF5, HDAC7, ID3, INTU, PDLIM7, PEX7, RHOA, RPL38, SFRP1, SIX2, SMAD1, SNAI1, SOX-9, and TMEM64) in the 3D-PLA loaded with the gingival MSC-CM group [172]. This was basically attributed to the growth factors and cytokines contained in the CM that could activate mobilization and osteogenic differentiation of both endogenous MSCs and gingival MSCs [28][29][30][31]172]. In a further study, EVs derived from human gingival MSCs were complexed with polyethyleneimine (PEI) to improve their internalization and performance.…”

Section: Gingival Msc-cm In the Therapy Of Neural Disordersmentioning

confidence: 97%

“…Among the advantages reported for stem/progenitor cell-derived secretome over cell-based therapy are its ease of preservation, sterilization, packaging, and storage for extended periods without the risk of losing its properties. It can be accurately gauged for proper dosages and produced in large quantities, using cell lines without subjecting the patient to invasive extraction procedures, which is both time and cost saving [28][29][30][31]. In this review, we aim to investigate the efficacy of secretome derived from various dental mesenchymal stem/progenitor cell (dental MSC) populations in the therapeutic approaches of various diseases as well as on different tissues' regeneration, highlighting the bioactive molecules involved in their action.…”

Section: Introductionmentioning

confidence: 99%

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Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Moshy

Radwan

Rady

et al. 2020

Stem Cells International

108

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Regenerative medicine literature has proposed mesenchymal stem/progenitor cell- (MSC-) mediated therapeutic approaches for their great potential in managing various diseases and tissue defects. Dental MSCs represent promising alternatives to nondental MSCs, owing to their ease of harvesting with minimally invasive procedures. Their mechanism of action has been attributed to their cell-to-cell contacts as well as to the paracrine effect of their secreted factors, namely, secretome. In this context, dental MSC-derived secretome/conditioned medium could represent a unique cell-free regenerative and therapeutic approach, with fascinating advantages over parent cells. This article reviews the application of different populations of dental MSC secretome/conditioned medium in in vitro and in vivo animal models, highlights their significant implementation in treating different tissue’ diseases, and clarifies the significant bioactive molecules involved in their regenerative potential. The analysis of these recent studies clearly indicate that dental MSCs’ secretome/conditioned medium could be effective in treating neural injuries, for dental tissue regeneration, in repairing bone defects, and in managing cardiovascular diseases, diabetes mellitus, hepatic regeneration, and skin injuries, through regulating anti-inflammatory, antiapoptotic, angiogenic, osteogenic, and neurogenic mediators.

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Section: Gingival Msc-cm In the Therapy Of Neural Disordersmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Moshy

Radwan

Rady

et al. 2020

Stem Cells International

108

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“…Extraction of the secretome from conditioned medium is more economical and practical than large-scale production and administration of stem cells [65]. Application of the secretome removes issues of immune compatibility, tumorigenicity, and infection transmission; it can be assessed similarly to current pharmaceutical agents; long-term storage without diminishing the potency can be achieved without common toxic cryopreservation agents, such as dimethyl sulfoxide (DMSO) [66][67][68]; and manufacturing schedules are significantly reduced in comparison to cell therapies. However, due to a cell's response to signalling affecting the composition of the secretome, producing an efficacious output is challenging [64,69].…”

Section: Innate Kidney Repair and The Influence Of Stem Cellsmentioning

confidence: 99%

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

2019

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The widening gap between organ availability and need is resulting in a worldwide crisis, particularly concerning kidney transplantation. Regenerative medicine options are becoming increasingly advanced and are taking advantage of progress in novel manufacturing techniques, including 3D bioprinting, to deliver potentially viable alternatives. Cell-integrated and wearable artificial kidneys aim to create convenient and efficient systems of filtration and restore elements of immunoregulatory function. Whilst preliminary clinical trials demonstrated promise, manufacturing and trial design issues and identification of suitable and sustainable cell sources have shown that more development is required for market progression. Tissue engineering and advances in biomanufacturing techniques offer potential solutions for organ shortages; however, due to the complex kidney structure, previous attempts have fallen short. With the recent development and progression of 3D bioprinting, cell positioning and resolution of material deposition in organ manufacture have never seen greater control. Cell sources for constructing kidney building blocks and populating both biologic and artificial scaffolds and matrices have been identified, but in vitro culturing and/or differentiation, in addition to maintaining phenotype and viability during and after lengthy and immature manufacturing processes, presents additional problems. For all techniques, significant process barriers, clinical pathway identification for translation of models to humans, scaffold material availability, and long-term biocompatibility need to be addressed prior to clinical realisation.

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“…MSC anti-inflammatory effect is mediated by cytokines such as TGF-β1 [142], IL-10, IL-12p70, IL-17E, IL-27 IL-13 [142,143], IL-1 receptor antagonist (IL1RA), IL-18 binding protein (IL-18BP), ciliary neurotrophic factor (CNTF), neurotrophin 3 (NT-3) factors [142,143], among others. On the other hand, MSC acellular derivatives have also been found to contain pro-inflammatory cytokines, such as IL-1b [142], IL-6 [144,145], IL-8 [146,147] and IL-9 [147], that are in balance with the anti-inflammatory cytokines, and this balance may determine the ultimate response in the tissue.…”

Section: Immunomodulation and Suppression Of Inflammationmentioning

confidence: 99%

“…On the other hand, MSC acellular derivatives have also been found to contain pro-inflammatory cytokines, such as IL-1b [142], IL-6 [144,145], IL-8 [146,147] and IL-9 [147], that are in balance with the anti-inflammatory cytokines, and this balance may determine the ultimate response in the tissue. Nevertheless, it is also remarkable that MSC acellular derivatives inhibit pro-inflammatory cytokines (for example, interferon (IFN) and TNFα), while increasing anti-inflammatory IL-10 release [143,148]. Specifically, Legaki et al reported that MSC acellular derivatives significantly reduced the mRNA expression of IL-6, IL-8, TNFα and macrophage inflammatory proteins 1(MIP-1), and increased the mRNA expression of the IL-10 anti-inflammatory cytokine [149].…”

Section: Immunomodulation and Suppression Of Inflammationmentioning

confidence: 99%

Function and Therapeutic Potential of Mesenchymal Stem Cells and Their Acellular Derivatives on Non-Healing Chronic Skin Ulcers

Solarte¹,

Becerra-Bayona²,

Sánchez-Aranguren³

et al. 2018

J Stem Cell Res Ther

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Non-healing chronic skin ulcers are considered a major biological, psychological, and financial burden for both patients and health systems. Multidisciplinary endeavors are required to address this refractory disease, in order to find definitive solutions that lead to improved living conditions. Diabetes, venous stasis, arterial insufficiency, pressure and radiation are common risk factors associated with chronic wounds. Unfortunately, the cured state for these wounds has a high relapse rate, which adversely affects the patient's quality of life. Nevertheless, advances on regenerative medicine have allowed the development of cell-based therapies that promote wound healing by increasing cell migration and differentiation. Particularly, mesenchymal stem cells (MSCs) and their acellular derivatives have emerged as an attractive therapeutic agent in various diseases, including chronic skin ulcers, due to their role in immunomodulation and tissue regeneration. In this review discusses the characteristics of MSCs as well as their regenerative properties and their action mechanisms on wound healing. Finally, the perspectives of MSCs and their acellular derivatives in clinical chronic skin ulcer therapy are also explored.

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Anti-inflammatory effect of conditioned medium from human uterine cervical stem cells in uveitis

Cited by 78 publications

References 27 publications

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

Function and Therapeutic Potential of Mesenchymal Stem Cells and Their Acellular Derivatives on Non-Healing Chronic Skin Ulcers

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